EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19370

Nupr1 nuclear protein 1 ( MGI:1891834)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002170. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002170_01	euxassay_002170_02	euxassay_002170_03	euxassay_002170_04

euxassay_002170_05	euxassay_002170_06	euxassay_002170_07	euxassay_002170_08	euxassay_002170_09

euxassay_002170_10	euxassay_002170_11	euxassay_002170_12	euxassay_002170_13	euxassay_002170_14

euxassay_002170_15	euxassay_002170_16	euxassay_002170_17	euxassay_002170_18	euxassay_002170_19

euxassay_002170_20	euxassay_002170_21	euxassay_002170_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19370_wholemount_strong.wlz

19370_wholemount_moderate.wlz

19370_wholemount_weak.wlz

19370_wholemount_possible.wlz

19370_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19370_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

strong

regional

strong expression: see section 07 08 09 10 12 13

meckel's cartilage

strong

regional

strong expression: see section 03 04 05 06 07 15 16 17 18 19 20 21

lower jaw incisor

strong

regional

strong expression: see section 08 09 13 14

lower jaw molar

strong

regional

strong expression: see section 04 05 06 07 16 17 18 19

upper jaw incisor

strong

regional

strong expression: see section 08 09 13 14

upper jaw molar

strong

regional

strong expression: see section 04 05 06 07 16 17 18 19

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6016
Entity Detected:	Nupr1, nuclear protein 1 ( MGI:1891834)
Sequence:	sense strand is shown >T6016 AGGAGACACAGGCAAGACTTTGGAGAGAGCAGACTAGGCATAATGGCCACCTTGCCACCAACAGCCAACC CTTCCCAGCAACCTCTAAACCTAGAGGATGAAGATGGAATCCTGGATGAATATGATCAGTACAGCCTGGC CCATCCCTGTGTCGTCGGGGGAGGTCGGAAAGGTCGGACCAAGAGAGAAGCTGCTGCCAATACCAACCGC CCTAGCCCTGGCGGGCATGAGAGGAAGCTGCTGACCAAGTTCCAGAACTCTGAAAGGAAAAAAGCCTGGC GCTGAGACGGAGCTGGAGATAAGGCCAGACCACAGACACCACACCCAGCAATGGATACAGGACATCACAC CCAGCAATGGATACAGGACATCACACCCAGCAATGGATACAGGACCTTGGAGAAATTAGGAGTTGTAAGC AGGACCTAGGCCTGCTTGATTCTTTCCCACCTAAGTGAGGTTCTGGAGAGGGAGCCCTTCCACCCTTCCA GACCCAGCAAAAGGGCAAGATGGGAGCAAGAAGCAGAGGGTAGACGGGCAGAGCTAGGAAGACAGCCCCC AAAAGAACTGTGGGGGAAATAAATCGGATGCCCTTGGGTGGCAGCAACAATAAATCGTACAGCAGTGAAA AAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3487434 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3487434 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19367 same embryo
	EMAGE:19368 same embryo
	EMAGE:19366 same embryo
	EMAGE:19365 same embryo
	EMAGE:19369 same embryo
	EurExpress:euxassay_002170 same experiment
	MGI:4826836 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source