EMAGE mouse gene expression spatial database

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EMAGE:19383

Fga fibrinogen alpha chain ( MGI:1316726)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002210. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002210_01	euxassay_002210_02	euxassay_002210_03	euxassay_002210_04

euxassay_002210_05	euxassay_002210_06	euxassay_002210_07	euxassay_002210_08	euxassay_002210_09

euxassay_002210_10	euxassay_002210_11	euxassay_002210_12	euxassay_002210_13	euxassay_002210_14

euxassay_002210_15	euxassay_002210_16	euxassay_002210_17	euxassay_002210_18	euxassay_002210_19

euxassay_002210_20	euxassay_002210_21	euxassay_002210_22	euxassay_002210_23	euxassay_002210_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19383_wholemount_strong.wlz

19383_wholemount_moderate.wlz

19383_wholemount_weak.wlz

19383_wholemount_possible.wlz

19383_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19383_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6020
Entity Detected:	Fga, fibrinogen alpha chain ( MGI:1316726)
Sequence:	sense strand is shown >T6020 CCCACGCGTCCGCTCCAGTTCTCCTCATGAGCCATCCCTAAACGCAGACACCATGCTGTCCCTGAGGGTC ACCTGCCTCATCTTGAGCGTGGCCAGCACAGTCTGGACTACAGATACCGAAGATAAAGGTGAATTCCTAT CAGAAGGAGGAGGAGTACGTGGCCCAAGAGTTGTGGAGAGACATCAGAGTCAATGCAAGGACTCAGACTG GCCCTTCTGCTCTGATGATGACTGGAACCACAAATGCCCTTCAGGCTGCAGGATGAAAGGGTTGATTGAC GAAGCCAACCAGGATTTTACAAACAGAATCAACAAGCTCAAAAACTCACTATTTGACTTTCAAAGGAACA ACAAGGATTCTAACTCACTGACCAGGAATATCATGGAGTATTTGAGAGGGGACTTCGCGAATGCCAACAA CTTTGATAACACTTATGGGCAAGTGTCAGAAGACCTGAGGCGCAGAATTGAGATCCTGAGGCGAAAAGTC ATAGAGAAAGCGCAACAGATTCAAGCTCTGCAGAGCAATGTCCGGGCTCAGTTGATAGACATGAAGCGCC TGGAGGTGGATATTGATATCAAGATCCGCTCTTGCAAAGGGTCCTGCAGCAGGGCTGTAAACCGTGAGAT AAATCTACAGGACTATGAAGGTCACCAAAAACAGCTTCAACAGGTCATCGCTAAAGAATTGCTTCCTACA AAAGACAGGCAGTACTTGCCAGCACTAAAAATGTCTCCAGTTCCCGACTTGGTTCCCGGAAGTTTTAAGA GCCAGCTTCAGGAGGCCCCTCCAGAGTGGAAGGCATTAACAGAAATGCGGCAGATGAGAATGGAGCTGGA GAGACCTGGGAAGGATGGGGGTTCGCGAGGAGATTCACCAGGAGACTCGCGGGGAGATTCTAGAGGGGAC TTTGCAACACGTGGACCAGGGTCAAAGGCAGAAAACCCCACGAACCCTGGACCTGGTGGATCTGGGTATT GGCGTCCTGGGAACTCCGGGTCTGGAAGTGATGGAAATCGGAACCCTGGGACCACGGGGTCTGATGGCAC TGGAGACTGGGGTACCGGAAGCCCTAGACCTGGCTCAGACTCTGGGAACTTTAGGCCTGCCAACCCTAAC TGGGGTGTGTTTTCAGAGTTTGGAGACAGTAGCAGCCCAGCCACAAGAAAAGAGTATCACACAGGTAAAG CGGTCACTTCTAAAGGAGATAAAGAGCTCCTGATTGGAAAGGAGAAAGTCACCTCTTCTGGCACAAGCAC CACACATCGTTCATGCTCTAAAACCATTACCAAGACTGTCACAGGTCCTGATGGTCGCCGAGAAGTGGTC AAAGAAGTGATCACCTCGGATGATGGCTCAGACTGTGGCGATGCCACCGAGTTAGACATATCCCACAGTT TTAGCGGCAGTCTCGACGAACTCTCTGAAAGGCATCCTGACCTTTCTGGGTTTTTTGACAACCACTTTGG TTTAATCTCACCTAACTTCAAAGAATTTGGCAGTAAGACCCATTCTGATTCCGACATCCTCACAAACATT GAGGACCCCAGCTCCCATGTACCTGAGTTTTCTTCCAGTAGTAAAACCTCAACTGTCAAAAAACAAGTAA CCAAGACCTATAAAATGGCAGACGAGGCAGGAAGCGAAGCTCACCGGGAAGGAGAAACTCGCAACACTAA GAGGGGCCGTGCCAGAGCTCGCCCAACGAGAGGTATCGACACTTAACTTTCGGGAGATTCCCCTGACACC CCCCCCCCCCCTAGATTAAGTTAACCATTACTGCGAAGTGCTTACCAGGCACGCTGCACCCGTTTCCTAA CCTCCTTTAGTGTTTTGTTGGAATCTCATTTTTTTTTTGGTCAACTTTTCATGCTAGACTGTACGTTCCT TGGGGGCAGGGACTTGACCATGTGTCTATTTCTGTAATTCCCAAATGCCTAACAGTGCAGTCATTTCTCA ATAAATACATTTTAAATAAATGAACAAATTCTGCCGAAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3487861 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3487861 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4824834 same experiment
	EurExpress:euxassay_002210 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source