EMAGE mouse gene expression spatial database

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EMAGE:19388

Cwc22 CWC22 spliceosome-associated protein homolog (S. cerevisiae) ( MGI:2136773)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002206. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002206_01	euxassay_002206_02	euxassay_002206_03	euxassay_002206_04

euxassay_002206_05	euxassay_002206_06	euxassay_002206_07	euxassay_002206_08	euxassay_002206_09

euxassay_002206_10	euxassay_002206_11	euxassay_002206_12	euxassay_002206_13	euxassay_002206_14

euxassay_002206_15	euxassay_002206_16	euxassay_002206_17	euxassay_002206_18	euxassay_002206_19

euxassay_002206_20	euxassay_002206_21	euxassay_002206_22	euxassay_002206_23	euxassay_002206_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19388_wholemount_strong.wlz

19388_wholemount_moderate.wlz

19388_wholemount_weak.wlz

19388_wholemount_possible.wlz

19388_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19388_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6083
Entity Detected:	Cwc22, CWC22 spliceosome-associated protein homolog (S. cerevisiae) ( MGI:2136773)
Sequence:	sense strand is shown >T6083 GCGCGGTGGAGCTTTAGGTGACGTGTAGAGACAGAAAAAGAAAACTATCGAGGAACTCAGACCACTGAGC ACGTGTTACTTGAATGAACCTGAAGAATCTGGGGCAGCTGACAGCAGAAAATGAAAAGTAGTGTGGCACA CATGAAGCAGTCCTCTGGTCACAACAGAAGGGAGACCCACAGTTCATACCGCCGGAGCTCCTCCCCAGAA GACAGATACACAGAGCAAGAGCGGTCCCCCCGGGATAGAGGTTACTCTGATTACAGCCGGTCAGACTATG AGCGATCTAGAAGAGGATACTCTTACGATGACAGCATGGAATCACGAAGTAGGGACCGAGAAAAACGCAG AGAGAGAGAGAGAGATGCAGATCATCGGAAACGGTCCCGGAAATCGCCATCCCCCGACAGGAGTCCAGCC CGAGGCGGAGGGCAGAGTTCTCCTCAGGAAGAACCGACATGGAAGAAGAAGAAGGATGAGCTGGACCCGC TGCTCACCCGCACTGGGGGAGCGTATATCCCTCCTGCGAAGCTCAGGATGATGCAGGAGCAGATCACAGA TAAAAGCAGCTTAGCATATCAGAGGATGAGCTGGGAGGCTCTGAAGAAATCCATCAATGGTCTCATCAAC AAGGTCAACATTTCTAATATAAGCATTATTATCCAAGAGCTCCTTCAAGAGAACATTGTCAGAGGGAGAG GATTGCTGTCCAGATCTGTTCTGCAAGCCCAGAGTGCGTCTCCAATCTTCACTCACGTTTATGCAGCTTT GGTGGCAATAATCAACTCAAAATTCCCACAGATTGGAGAATTGATCCTCAAGAGACTAATTCTTAATTTT CGGAAAGGCTATCGAAGGAACGATAAGCAACTTTGCCTGACTGCTTCAAAATTTGTGGCACATCTTATTA ACCAAAATGTGGCACATGAGGTTCTGTGCCTGGAGATGCTCACTTTGCTACTAGAGAGACCAACAGATGA CAGTGTTGAAGTAGCTATCGGCTTCCTCAAAGAGTGTGGTCTCAAATTAACCCAGGTGTCACCAAGGGGA ATTAATGCAATCTTTGAACGCCTTCGAAACATTCTACACGAGTCTGAGATCGACAAAAGAGTTCAGTACA TGATTGAAGTGATGTTTGCTGTACGGAAGGATGGATTCAAGGACCACCCTGTTATTCTGGAAGGACTTGA TTTAGTGGAAGAGGACGATCAGTTTACACATATGCTCCCCTTAGAGGATGACTACAATCCAGAAGATGTT CTTAATGTTTTCAAGATGGATCCTAATTTTATGGAGAATGAAGAGAAGTACAAGGCTATTAAAAAAGAAA TCCTTGACGAAGGAGACAGTGATTCAAACACAGACCAAGGAGCTGGAAGTAGTGAAGACGAGGAGGAGGA AGACGAGGAGGAGGAAGGAGAAGACGAGGAAGGAGGGCAAAAAGTAACTATTCATGACAAAACAGAAATC AACCTAGTCTCGTTTCGCCGTACAATCTATCTTGCCATTCAGTCAAGTTTAGATTTTGAAGAATGTGCAC ACAAGTTGCTGAAAATGGAGTTTGCAGAGAGCCAAACAAAAGAACTCTGCAACATGATACCTGATTGCTG TGCCCAACAAAGGACCTATGAAAAATTTTTTGGCTTGTTAGCTGGGCGGTTTTGTATGCTGAAAAAGGAA TACATGGAATCATTTGAAAGTATATTCAAGGAACAGTACGACACCATCCATCGCTTAGAGATAAACAAAT TGAGAAATGTTGCTAAGATGTTTGCTCATCTTTTATACACAGATTCACTTCCGTGGAGTGTTCTCGAATG TATAAAACTGAGTGAAGAGACCACGACATCGTCTAGTCGAATTTTTGTCAAGATCTTCTTCCAGGAACTG TGTGAATACATGGGTCTTCCTAAACTGAATGCGAGGTTAAAGGATGAAACTCTCCAGCCATTCTTTGAAG GTCTGTTACCCAGAGACAACCCACGCAATACCCGGTTTGCCATCAACTTCTTCACTTCCATAGGTCTCGG AGGATTGACGGATGAACTGCGAGAACATCTGAAAAATACACCCAAGGTCATCGTAGCCCAGAAACCAGAA GCTGAACAAAAGAAACCAGCCCTGACATCTTCATCTTCAGAGTCTTCCTCGGCGTCTGATTCCTCTGACT CAGAATCTGACAGCAGTGAGAGCAGCTCAGAGTCCTCTAGTGACGCGAGCGACTCCTCCTCCTCCTCCTC CAGCACTCAGAGTTCTACCTCAGCTAAAGGTACAAGAAAGAAGAGACAAGGGAAGGCCAGAGGCGAAGAA GTTGATAAGTTAGCCAGGGGCCACCAGGCACTTGAGCGGAGGCAAGAAGGAGGCAGGGAGGACCAAAGGC ACCAGGAAGGGAGGACTGAGAGAGCAAGGTCTGAAAGACGCAGAGCTCAGAACTCACGAGATGCAGACTG GAGAGATCCCCTAGCAAAGCACATAGACGACAGAAGCCATGAGAATAGTCACAGCAGAGTTGGCAATGGC CGAGAACAAGGCTCACACAGAGAGCCAGAAGACAGGCATGGCGAGCCAAAGAAGCGGAGAGAGAGAAGAG ATTCTTTTTCTGAAAATGAGAAGCAAAGAAGCCGAAATCAGGACAGTGATAATGTTAGAAGAAAAGATCG CTCCAAGTCAAGGGAGAGGAGTAGAAGGCATTCTGGTCACAAGGGAGATGACGCCAGGTGTCAAAATGGT GCAGAGAGGCGCTGGGAAAAGCCTGGCAGACGCCCTGAACAGTCTAGAGAGTCAAAGAGAAGTCAGGACC GGAGGAGAGAAAAGTCTCCAACAACACAGAAATAATCCTAATGACAACTGAACATGTCTTACACTTAATT TAGTTCAGTGTCTTTTTTACATTGATGAATTTTATAGAGAACTCTTGTATAAAGTACCTGTTTGTAAATT TTGAAGATGTTTTCTTTTTGACATGTTTTATAATTGATATATCTCAAGGTTCTTTGTGTAAAATCATAAA GAAAAGGGATTCAACAAAAGGGTAAGTCTTATGTTTTGTGAATAAAAGGAACAAATGTTCATTGAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3492146 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3492146 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19385 same embryo
	EMAGE:19386 same embryo
	EMAGE:19387 same embryo
	EMAGE:19384 same embryo
	EurExpress:euxassay_002206 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source