EMAGE mouse gene expression spatial database

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EMAGE:19420

Prpf3 PRP3 pre-mRNA processing factor 3 homolog (yeast) ( MGI:1918017)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005024. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005024_01	euxassay_005024_02	euxassay_005024_03	euxassay_005024_04

euxassay_005024_05	euxassay_005024_06	euxassay_005024_07	euxassay_005024_08	euxassay_005024_09

euxassay_005024_10	euxassay_005024_11	euxassay_005024_12	euxassay_005024_13	euxassay_005024_14

euxassay_005024_15	euxassay_005024_16	euxassay_005024_17	euxassay_005024_18	euxassay_005024_19

euxassay_005024_20	euxassay_005024_21	euxassay_005024_22	euxassay_005024_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19420_wholemount_strong.wlz

19420_wholemount_moderate.wlz

19420_wholemount_weak.wlz

19420_wholemount_possible.wlz

19420_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19420_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6502
Entity Detected:	Prpf3, PRP3 pre-mRNA processing factor 3 homolog (yeast) ( MGI:1918017)
Sequence:	sense strand is shown >T6502 CAGGGGCTGAAGTTTGTGAGGTGTAGTTTTTTGTCCCATTGGAGCTCTCATTCTCCATTTTCTCCGAAAT GGCGCTGTCTAAGCGGGAGCTGGATGAGCTGAAGCCATGGATAGAGAAGACGGTGAAGAGAGTGCTGGGC TTCTCAGAGCCCACAGTGGTCACTGCAGCCTTGAACTGTGTGGGGAAGGGCATGGACAAAAAGAAGGCGG CTGATCACCTGAAACCTTTTCTTGATGATTCCACTCTCCGATTTGTGGATAAGCTGTTTGAGGCTGTGGA GGAAGGCCGAAGCTCAAGGCATTCCAAGTCTAGTAGTGACAGAAGCAGAAAGCGAGAGTTAAAGGAGGTG TTTGGTGATGACTCTGAGATCTCAAAGGAATCATCGGGGGTAAAGAAGCGGCGGATACCCCGTTTTGAGG AGGTAGAAGAGGAGCCAGAGGTGATCCCTGGACCTCCATCAGAAAGCCCTGGCATGCTAACAAAGCTCCA GATCAAACAGATGATGGAAGCAGCAACACGACAAATCGAGGAACGGAAAAAACAGCTGAGCTTCATCAGC CCCCCTGCACCTCAGCCAAAGACACCTTCTTCTTCCCAACCTGAGCGACTTCCCATCGGCAACACTATTC AGCCCTCCCAGGCCGCTACCTTTATGAACGATGCCATTGAGAAGGCGAGGAAAGCTGCCGAGCTGCAAGC CCGAATCCAGGCCCAGCTGGCATTGAAGCCAGGGCTTATTGGCAATGCCAACATGGTGGGCCTAGCTAAT CTCCATGCCATGGGCATAGCTCCACCGAAAGTAGAGTTAAAAGACCAAACTAAGCCTACCCCATTGATCC TGGACGAGCAAGGCCGCACTGTGGATGCAACAGGCAAGGAGGTGGAGCTCACACATCGCATGCCCACCCT GAAGGCAAACATTCGGGCTGTGAAGAGGGAACAGTTCAAGCAGCAGCTAAAAGAAAAGCCATCAGAAGAC ATGGAGTCTAATACCTTTTTTGACCCCCGAGTCTCAATTGCCCCTTCCCAGCGCCAAAGACGCACTTTTA AATTCCATGACAAGGGCAAATTTGAGAAAATTGCTCAACGATTACGGACAAAGGCTCAATTGGAGAAGCT GCAAGCAGAGATTTCACAAGCTGCTAGAAAAACAGGCATTCATACTTCGACTAGACTTGCCCTCATTGCT CCCAAAAAGGAGCTAAAGGAAGGCGATATCCCTGAGATTGAGTGGTGGGACTCGTACATCATACCCAACG GCTTTGACCTTACAGAGGAAAATCCCAAGAGAGAAGATTATTTTGGAATCACAAATCTTGTTGAACATCC AGCCCAGCTTAATCCTCCAGTTGACAATGATACACCAGTTACCCTTGGGGTATATCTTACGAAGAAGGAG CAGAAGAAACTTCGGAGGCAAACAAGAAGGGAAGCACAGAAAGAGCTACAAGAGAAAGTCAGGCTAGGCC TGACACCTCCTCCAGAGCCCAAAGTGAGAATCTCAAATTTAATGCGAGTATTAGGAACAGAAGCAGTTCA AGACCCTACAAAGGTAGAAGCCCATGTCAGAGCTCAGATGGCAAAGAGACAGAAAGCGCATGAAGAGGCC AACGCGGCCCGAAAACTTACAGCAGAACAGAGAAAGGTCAAGAAAATTAAAAAGCTTAAAGAAGACATTT CACAGGGGGTGCACATATCTGTGTATAGAGTTCGAAATTTGAGCAACCCAGCCAAGAAGTTCAAGATTGA GGCCAATGCTGGGCAGCTGTACCTGACAGGGGTGGTGGTGCTGCACAAAGATGTCAACGTGGTGGTAGTG GAAGGGGGCCCCAAAGCCCAGAAGAAATTTAAGCGTCTCATGCTACATCGGATAAAGTGGGATGAGCAGA CATCTAACACGAAGGGAGATGATGATGAGGAATCTGATGAAGAAGCTGTGAAGAAAACTAACAAATGTGT ACTAGTATGGGAGGGTACAGCCAAAGACCGGAGCTTTGGAGAGATGAAGTTTAAACAGTGCCCTACAGAG AACATGGCTCGTGAGCATTTCAAAAAGCATGGGGCTGAACACTACTGGGACCTTGCACTGAGTGAATCTG TGTTGGAGTCCACCGACTGAGACTACTGCTAGCCCTTGTATCTTCTTTGCCTTTGTTTCTTCTGTCTTCT CACTCTACTTCCCAACCTTACCCCACCGTGCGTGTGATCTCAGAACTGTGCCAGGCCGATACCAGAGCAA AAAGACTACCAAGTTCACCCAGTCAGCCTGGTGCTACCCTCCATCCTGTATGTGCATATAATTAAAGAGT TATTTTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3589226 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3589226 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19424 same embryo
	EMAGE:19419 same embryo
	EMAGE:19422 same embryo
	EMAGE:19423 same embryo
	EMAGE:19421 same embryo
	EurExpress:euxassay_005024 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source