EMAGE mouse gene expression spatial database

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EMAGE:19421

Nmt1 N-myristoyltransferase 1 ( MGI:102579)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005020. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005020_01	euxassay_005020_02	euxassay_005020_03	euxassay_005020_04

euxassay_005020_05	euxassay_005020_06	euxassay_005020_07	euxassay_005020_08	euxassay_005020_09

euxassay_005020_10	euxassay_005020_11	euxassay_005020_12	euxassay_005020_13	euxassay_005020_14

euxassay_005020_15	euxassay_005020_16	euxassay_005020_17	euxassay_005020_18	euxassay_005020_19

euxassay_005020_20	euxassay_005020_21	euxassay_005020_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19421_wholemount_strong.wlz

19421_wholemount_moderate.wlz

19421_wholemount_weak.wlz

19421_wholemount_possible.wlz

19421_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19421_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6507
Entity Detected:	Nmt1, N-myristoyltransferase 1 ( MGI:102579)
Sequence:	sense strand is shown >T6507 GCAACCCAAGATGGCGGATGAGAGTGAGACAGCAGTGAAGCTGCCGGCACCTTCGTTGCCACTGATGATG GAAGGGAATGGAAACGGCCATGAGCACTGCAGCGATTGCGAGAACGAGGAGGACAACAGCCACAACAGGA GTGGCTTGAGTCCAGCCAATGACACCGGAGCCAAAAAGAAGAAAAAGAAACAAAAGAAGAAGAAAGAGAA AGGCAGTGACATGGAATCCACCCAGGACCAGCCCGTGAAGATGACCTCTTTGCCAGCAGAGAGGATCCAG GAAATACAGAAGGCCATTGAGCTGTTCTCAGTGGGTCAGGGACCCGCCAAAACCATGGAGGAAGCCAGTA AACGGAGCTACCAGTTCTGGGATACACAGCCAGTGCCCAAATTAGGTGAAGTGGTAAACACCCATGGTCC AGTCGAACCTGACAAAGACAACATTCGCCAGGAGCCCTACACCCTGCCCCAGGGCTTCACCTGGGATGCC TTGGATCTGGGAGACCGTGGTGTGCTGAAAGAGCTGTACACCCTCCTGAACGAGAACTACGTGGAAGATG ATGACAATATGTTCCGATTCGACTATTCCCCAGAGTTTCTCTTGTGGGCTCTCCGGCCACCAGGTTGGCT CCCACAGTGGCACTGTGGGGTCCGAGTGGTCTCAAGTCGGAAACTGGTTGGGTTCATCAGTGCCATCCCA GCAAACATCCACATCTATGACACAGAGAAGAAGATGGTGGAGATCAACTTCCTGTGCGTCCACAAGAAGC TGCGCTCTAAGAGGGTGGCTCCAGTTTTGATCCGAGAGATCACCCGGCGGGTCCACCTGGAGGGCATTTT CCAAGCTGTTTACACTGCCGGAGTGGTGTTGCCAAAGCCTGTGGGCACCTGCAGGTACTGGCATCGATCC CTAAACCCTCGGAAGCTGATTGAAGTGAAGTTCTCCCACCTGAGCAGAAATATGACCATGCAGCGCACCA TGAAGCTCTACCGACTGCCAGAGACTCCCAAGACAGCTGGCCTACGACCAATGGAGAAGAAGGACATTCC AGTAGTGCACCAACTGCTCAGCAGGTACCTGAAGCAGTTTCACCTCACGCCTGTCATGAACCAGGAGGAG GTGGAGCACTGGTTCTACCCCCAGGAGAACATCATTGACACTTTTGTGGTAGAGAATGCCAACGGCGAGG TGACTGATTTCCTGAGCTTTTATACACTTCCCTCCACCATCATGAACCATCCAACCCACAAGAGTCTGAA AGCTGCGTACTCCTTCTACAACGTCCACACCCAGACCCCTCTTCTAGACCTTATGAGCGACGCCCTCGTT CTAGCCAAAATGAAAGGGTTCGACGTATTCAATGCACTGGATCTCATGGAGAACAAGACATTCCTGGAGA AGCTCAAGTTCGGCATAGGGGACGGCAACCTGCAGTATTACCTGTACAATTGGAAGTGCCCGAGCATGGG GGCCGAGAAGGTTGGGTTGGTGTTACAGTAACCAGTTGCCAGTGAGATTCTGGATAAAGCCACTGAGAAT TCAAACCAGGAAACGGAACCCTTCCACTTGTTGGTCCAACTTTCACAGATGTGAGATCTCTGGCAAACAA AACAGAACTGAACCGGCTTTACCAAACCGCCAGCGAACTTGACAATTATATTGCGATGGCGTAGGCTGCG TGATGTCACCTTTAGCCATGTTTCTGGTCTCCCTATTTTCAATGAATTAACATGCTCATGCAGCCGTGAT CAAGGGAATGTAACTGCTGAAACCTAGCTCGTGATTGGCATATTATGGAGTTAACGGGTGAATAATAAAA GTATATATATTATATATAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3589678 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3589678 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19424 same embryo
	EMAGE:19419 same embryo
	EMAGE:19422 same embryo
	EMAGE:19423 same embryo
	EMAGE:19420 same embryo
	EurExpress:euxassay_005020 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source