EMAGE mouse gene expression spatial database

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EMAGE:19422

Tmem184c transmembrane protein 184C ( MGI:2384562)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004841. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004841_01	euxassay_004841_02	euxassay_004841_03	euxassay_004841_04

euxassay_004841_05	euxassay_004841_06	euxassay_004841_07	euxassay_004841_08	euxassay_004841_09

euxassay_004841_10	euxassay_004841_11	euxassay_004841_12	euxassay_004841_13	euxassay_004841_14

euxassay_004841_15	euxassay_004841_16	euxassay_004841_17	euxassay_004841_18	euxassay_004841_19

euxassay_004841_20	euxassay_004841_21	euxassay_004841_22	euxassay_004841_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19422_wholemount_strong.wlz

19422_wholemount_moderate.wlz

19422_wholemount_weak.wlz

19422_wholemount_possible.wlz

19422_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19422_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 15

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 15 16 17 18 19 20

vagus x ganglion

strong

regional

strong expression: see section 05 14

spinal cord

strong

homogeneous

strong expression: see section 05 06 07 08 09 10 11 12 13

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 05 06 12

cervical ganglion

moderate

regional

moderate expression: see section 06 13

dorsal root ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 11 12 13 14

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 11 13 14 15 16 17

lower jaw incisor

weak

regional

weak expression: see section 09 10 14

upper jaw incisor

weak

regional

weak expression: see section 10 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6505
Entity Detected:	Tmem184c, transmembrane protein 184C ( MGI:2384562)
Sequence:	sense strand is shown >T6505 CCCACGCGTCCGCGGACGCGTGGGTGGTAGGGTACCAGATGCAGGTCAGACCCCGAGTGCCTAGAATCCC TAGTTTACCCTGATAATCCTCGGGGTCACGATGCAGCGGAGACTCTGGGTGACCTCCAGGTGAGGCGGAG GCGACGCCGCGGCGGCACTGAGTCCATGCGGGTTGGCCGGCGAGGATGCAGCCTGCACCTGCGAGCATCC CGAGCGGCCACCGCTGCAGCAGCCGCAAACACAGAGCGACCCGAGAACGCGGCGCCAGCGGCTAGTAAAT CTTTTCCTATAGGGCGCCAGCGGAGAAGACAACCGAAAAAGCGAACTTGCCAAATTCTTGGTTTGGTTCG ATTATTCAGCTACTCAAAGAGAATCTGTTACAGAACATGCCTTGCGCATGTAATCGGAGTAACTGGAGGA GATGGATCCGACCCCTGTTGGTACTTTTTTACGCAACAACCATCCTGGTCGCGGTTCCGATATGCATTTG GAAGTTTCAAAAAATGAAGGTTGGAATGCACACGAAATCCTGGTTTATAGCTGGAATATTTCTGCTGCTA ACCATACCCGTGTCATTGTGGGGAATCCTTCAACATCTGGTACATTACACTCAACCTGAACTTCAAAAGC CCATAATAAGGATCCTATGGATGGTCCCAATATACAGTGTGGATAGTTGGGTGGCCTTGGTATATCCCAA AATTGCAATATACGTGGATACCTGGAGGGAGTGTTACGAAGCTTACGTCATCTATAACTTTATGATCTTT CTTACCAACTATCTGACAATCCGATTCCCAAACTTGATATTACACCTCGAAGCGAAAGATCAACAAAACC ACATCCTTCCGTTATGCTGCTGTCCACCGTGGGCGATGGGAGAAATGCTACTCTTTCGTTGCAAGCTAGG AGTGCTGCAATATACCGTGGTCAGACCGATCACCACCGTCACTGCACTCGTATGTGAAATCCTTGACGTA TATGATGAAGGAAACTTTGGTTTCAGCAATGCTTGGACATACCTCGTTATACTAAATAATTTGTCACAAT TGTTTGCCATGTACTGCCTGCTGCTGTTTTACAAAGTCCTAAAGGAAGAGCTTAGCCCTATACAACCTGT TGGCAAGTTCCTCTGCGTAAAACTTGTGGTTTTCGTTTCTTTTTGGCAAGCAGTACTTATTGCTTTGTTG GTTAAACTGGGCGTTATTTCAGAAAAGCGTACCTGGGAATGGCAAAGTGCAGAAGCTGTGGCCACGGGCC TGCAGGATTTCATAATCTGCATTGAAATGTTCTTTGCGGCAATTGCCCACCACTATACGTTCTCCTATAA ACCCTATGTGCATGAAGCAGAGGAAGGCTCCTGCTTTGACTCGTTTCTTGCAATGTGGGATGTGTCCGAT ATCAGAGATGATATTTCTGAACAAGTAAGGCGTGTCGGGAGGACAATGAGAGGTTATCCAAAAAAGAAAT GTTTTCCTGGAGATCCAGATCATAATGAGCACTCGAGCCTACTTTCTTCTTCTTCACAAGATCTAACTTC TGGTTCTTCAAAGGTACCCTCCCCAGGGGGCCTGTACCAAGGTTTTGGACACACTATTTCTTCACAGAGC CCAATTTCTATAGCCAGCATATATGAAGAGATCATGAATGACATCCCAGAAGAGCAACAGAAGCTTCTCA ATCCCGGACAAGACGTCACGATCAACATCCCAGAAGAGCAACAAAAACTTAACACAGGCAAGGATGTCAT GCTTGACAGACAGACCATTACTTCACCGAGACCAATTTCTATAGCCAGCATATATGAAGAGATCATGATT GACATCCCAGAAGAGCAACAAAAACTTACTGACACAGGCCAAGATGTCATGATTAACAGACAGACCATTA CTCCACCGAGACCAATTTCTATAACCAGCATATATGAAGAGGTCTTAATTGACATCACAGAAAAGCAACA AAAACTTAATGATACAGGCAAAAATGTCATGATTAACATCCCAGAAGAGCAACAAAAACTTATTGACAAA CGCAAAGATGTCATGATTGACATCCCAGAACAGAACGCAATTCCTGATAATAGCCAATATCAAGACCAGG AACAAATAGTTACTTTACAGGCTTTATTTCCTTCAACAGAAACATCTGAAAATAGCATGATTGACACCTC GGAATCACAGCAGGAGTCTTCTGATTTATGTACCGAATCTTCTGATTCATCTACCGAGTCTTCTGACCTA TCTACGGACCCTTAAAGCTGACATGTTAGCTCAAGATCTCTGTGCAGTTCAAAATGTTGCAAAATCTCAA AGATAGAAAAAACCACATATAATACTAATAACGCACATGTTAACATGCATATATTTGTATATGTTTTACA TATTACAAATAATGTTTAAATTTCCTAGGCTTTGACTTTCTTAAACATTTGGAATATCATATATTCAAAA AGTAAGTAACAACTTTAGCAAAATGCTCTTGAATTTATCAAACAGACAAACATAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3589819 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3589819 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19424 same embryo
	EMAGE:19419 same embryo
	EMAGE:19423 same embryo
	EMAGE:19421 same embryo
	EMAGE:19420 same embryo
	EurExpress:euxassay_004841 same experiment
	MGI:4828792 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source