EMAGE mouse gene expression spatial database

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EMAGE:19431

Slc35a4 solute carrier family 35, member A4 ( MGI:1915093)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004834. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004834_01	euxassay_004834_02	euxassay_004834_03	euxassay_004834_04

euxassay_004834_05	euxassay_004834_06	euxassay_004834_07	euxassay_004834_08	euxassay_004834_09

euxassay_004834_10	euxassay_004834_11	euxassay_004834_12	euxassay_004834_13	euxassay_004834_14

euxassay_004834_15	euxassay_004834_16	euxassay_004834_17	euxassay_004834_18	euxassay_004834_19

euxassay_004834_20	euxassay_004834_21	euxassay_004834_22	euxassay_004834_23	euxassay_004834_24

euxassay_004834_25	euxassay_004834_26	euxassay_004834_27

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19431_wholemount_strong.wlz

19431_wholemount_moderate.wlz

19431_wholemount_weak.wlz

19431_wholemount_possible.wlz

19431_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19431_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 14 15 16 17 18 19 20 21

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 08 12 13 18 19

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 10 11 12 13 14 16 17 18 19 20 21 22 23 24

cranium

moderate

regional

moderate expression: see section 01 02 03 07 08 09 23 24 25 26 weak expression: see section 04 05 06

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6525
Entity Detected:	Slc35a4, solute carrier family 35, member A4 ( MGI:1915093)
Sequence:	sense strand is shown >T6525 GGATGACAAGGATTCTCTGCCCAAGCTTAAGGACCTGACATTTCTCAAGAACCAGCTGGAGCGCCTACAG CAGCGTGTGGAAGGTGAAGTCAACAGTGGCGTAGGCCAGGATGGCTCCCTCTTGTCCTCCCCATTCTTCA AGGGCTTCCTGGCAGGATACGTGGTGGCCAAACTGAGGGCATCAGCAGTATTGGGCTTTGCGGTGGGCAC TTGCACTGGCATCTATGCAGCTCAGGCATATGCCGTACCCAACGTGGAGAAGGCACTGAAGAACTACTTT AGGTCACTACGGAAGGGGCCTGACTAGACCCTTTGGGGAAAGCAGGACGAGCATGTTGGGCCTGCAGGTA GAGCCCTTTCTATCACAAACACTCTATCTGGCTTCCCCTGCTATTATTGTTCATGTGCTAACTCCGTCTA TTCTGCCTCTTTCATCCCTGCCTTTCTTCCTGCAGAGAGTGGACAGTGCTTTGTCAGCCTGCACCCCAGA CTCCTTCCCTCCCTAGTTCCATGGGACTGACCCCAAAACTATGGGCCATTAATCCCCTCTTGAAGACCTA ACTGGAATGTGCGGCTGTCTCTGATCCTCAGTATTCTCTCTGGTGATGTACCACAGTTCTATACTCAATC TCCTACAAATGTGTCCCAGCCCCTCTGTCCCTTGCTAGCTACCCAGGCCACCTAGGGTCTGGTGTGGGCC ATGAGTGTAGAAGATGGGGGCGTGCCAGGCCTAGCCCGCCCAAGACAGGCTCGCTGGACCCTGTTGCTCT TCCTGTCCACTGCCATGTATGGTGCCCATGCACCGTTCTTAGCACTGTGCCATGTGGATGGCCGAGTGCC CTTCCGGCCCTCCTCAGCTGTGTTACTCACTGAGCTGACCAAGCTCCTGTTGTGCGCCTTCTCCCTCCTG GTAGGCTGGCAAACATGGCCCCAGGGCACGCCACCCTGGCGCCAGGCTGTGCCTTTTGCACTGTCAGCCC TGCTCTATGGCGCCAACAACAACCTGGTGATTTATCTGCAGCGCTACATGGACCCCAGCACCTATCAGGT GCTGAGCAATCTCAAGATTGGAAGCACAGCTCTATTGTACTGCCTCTGCCTTGGGCATCGTCTCTCTGCG CGTCAGGGCTTGGCGCTGCTGCTGCTGATGGCTGCAGGAGCCTGCTATGCATCAGGTGGCTTTCAGGAAC CTGTGAACACCCTTCCTGGGCCACCCGCGTCAGCAGCTGGAGCCCATCCCATGCCCTTGCATATCACTCC ACTGGGACTTCTGCTCCTCATCCTATACTGCCTCATCTCCGGCTTGTCCTCCGTGTACACAGAGCTGATC ATGAAGCGACAGCGGTTGCCCTTGGCTCTTCAGAACCTCTTCCTCTACACTTTTGGGGTGATCCTGAACC TTGGACTGTATGCTGGCAGTGGCCCAGGCCCGGGCTTCCTGGAGGGCTTCTCTGGATGGGCAGTGCTTGT GGTGCTGAACCAAGCAGTCAATGGGCTGCTCATGTCGGCTGTCATGAAGCATGGCAGCAGCATCACACGC CTCTTCATCGTGTCCTGCTCGCTCGTGGTCAACGCTGTGCTGTCGGCGGTGCTGCTCCAGCTGCAGCTCA CGGCCATCTTCTTCCTGGCCGCACTGCTCATCGGTCTGGCTGTGTGCTTGTACTATGGTAGCCCCTAAGT CCTTGACCACCTCCACTCACTTGTGACTGTGTAGATTAAATGCAACCACCAGAGTTACCTCCCATCCCCC TGTAATCCCCAGCAGCCCCGTAACAAGTGCCTTGTAAGAGAAGCTAAGGTGTCCAGGTTAGCCTTTGCGT GTTCAGGTGGGAGAGTTACCCCTAGGAGGCATAAAGAGTGGGCTTGGTTAAGGAAACGCCTAACTACACT CCTGAATTCTTTCGTACTCAAGAGGTGGGATCCCCAAACCCAGGCCTGTCCTTGAAGATCCCTGCCTTGT CCTGCATGTACATGGCTGCTTCAAGTGAAGCTTCAGTCTTCTTACCTGCTGTGGTCACTCCTGAAGACTC TGTCCTGAAGTTTGGTGCTGGCTGCTCCAGCCCAGCATGACTTCCATATAAAGATGCTTAGTTTGCCCCC TCCACAGAGCTGTTCCCCTCACCCACCTGTTATTCTGGAGGGGTCAGAGGAGGCCGGGGCTTGACTCATC TCAGGGAACATTGGCCCTGGGCCCTGGCTTAAGCCTACACTCCTGACCCCTGTGCTAACTCGAGAGCCCT GTTGAAGCCTTTCCCTCTGAAGCACTTAGGAGCTACTGCTCTTTCCACTCTCCGTGGTGTCCTCGCTTCC AACAGCTCGGGAGGGCTCTGCAGAGTAACCAGGGACCAGGTCCAGAAAATGTTTATAGCCAATCAGTGTC TAACTACTTCAGCAATAAGGTCTTAATAAAGTGTTCTAGGGTGTGGGGTGCTTCCTACAACCAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3590779 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3590779 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19433 same embryo
	EMAGE:19432 same embryo
	EurExpress:euxassay_004834 same experiment
	MGI:4828196 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source