EMAGE mouse gene expression spatial database

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EMAGE:19464

Nrp1 neuropilin 1 ( MGI:106206)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005158. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005158_01	euxassay_005158_02	euxassay_005158_03	euxassay_005158_04

euxassay_005158_05	euxassay_005158_06	euxassay_005158_07	euxassay_005158_08	euxassay_005158_09

euxassay_005158_10	euxassay_005158_11	euxassay_005158_12	euxassay_005158_13	euxassay_005158_14

euxassay_005158_15	euxassay_005158_16	euxassay_005158_17	euxassay_005158_18	euxassay_005158_19

euxassay_005158_20	euxassay_005158_21	euxassay_005158_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19464_wholemount_strong.wlz

19464_wholemount_moderate.wlz

19464_wholemount_weak.wlz

19464_wholemount_possible.wlz

19464_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19464_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8809
Entity Detected:	Nrp1, neuropilin 1 ( MGI:106206)
Sequence:	sense strand is shown >T8809 GGAAGAGGAACAAGTGTGGGAAAAGGGAGAGGAAATCGGAGCTAAATGACAGGATGCAGGCGACTTGAGA CACAAAAAGAAAAGCGCTTCTCGCGAATTCAGGCATTGCCTCGCCGCTAGCCTTCCCCGCCAAGACCCGC TGAGGATTTTCTGGTTCTTAGGCGGACTTAAGAGCGTTTCGGATTGTTAAGATTATCGTTTGCTGGTTTT TCGTCCGCGCAATCGTGTTCTCCTGCGGCTGCCTGGGGACTGGCTTGGCGAAGGAGGATGGAGAGGGGGC TGCCGTTGCTGTGCGCCACGCTCGCCCTTGCCCTCGCCCTGGCGGGCGCTTTCCGCAGCGACAAATGTGG CGGGACCATAAAAATCGAAAACCCAGGGTACCTCACATCTCCCGGTTACCCTCATTCTTACCATCCAAGT GAGAAGTGTGAATGGCTAATCCAAGCTCCGGAACCCTACCAGAGAATCATGATCAACTTCAACCCACATT TCGATTTGGAGGACAGAGACTGCAAGTATGACTACGTGGAAGTAATCGATGGGGAGAATGAAGGCGGCCG CCTGTGGGGGAAGTTCTGTGGGAAGATTGCACCTTCTCCTGTGGTGTCTTCAGGGCCCTTTCTCTTCATC AAATTTGTCTCTGACTATGAGACACATGGGGCAGGGTTTTCCATCCGCTATGAAATCTTCAAGAGAGGGC CCGAATGTTCTCAGAACTATACAGCACCTACTGGAGTGATAAAGTCCCCTGGGTTCCCTGAAAAATACCC CAACAGCTTGGAGTGCACCTACATCATCTTTGCACCAAAGATGTCTGAGATAATCCTGGAGTTTGAAAGT TTTGACCTGGAGCAAGACTCGAATCCTCCCGGAGGAATGTTCTGTCGCTATGACCGGCTGGAGATCTGGG ATGGATTCCCTGAAGTTGGCCCTCACATTGGGCGTTATTGTGGGCAGAAAACTCCTGGCCGGATCCGCTC CTCTTCAGGCGTTCTATCCATGGTCTTTTACACTGACAGCGCAATAGCAAAAGAAGGTTTCTCAGCCAAC TACAGTGTGCTACAGAGCAGCATCTCTGAAGATTTTAAGTGTATGGAGGCTCTGGGCATGGAATCTGGAG AGATCCATTCTGATCAGATCACTGCATCTTCACAGTATGGTACCAACTGGTCTGTAGAGCGCTCCCGCCT GAACTACCCTGAAAATGGGTGGACTCCAGGAGAAGACTCCTACAAGGAGTGGATCCAGGTGGACTTGGGC CTCCTGCGATTCGTTACTGCTGTAGGGACACAGGGTGCCATTTCCAAGGAAACCAAGAAGAAATATTATG TCAAGACTTACAGAGTAGACATCAGCTCCAACGGAGAGGACTGGATCTCCCTGAAAGAGGGAAATAAAGC CATTATCTTTCAGGGAAACACCAACCCCACAGATGTTGTCTTAGGAGTTTTCTCCAAACCACTGATAACT CGATTTGTCCGAATCAAACCTGTATCCTGGGAAACTGGTATATCTATGAGATTTGAAGTTTATGGCTGCA AGATAACAGATTATCCTTGCTCTGGAATGTTGGGCATGGTGTCTGGACTTATTTCAGACTCCCAGATTAC AGCATCCAATCAAGCCGACAGGAATTGGATGCCAGAAAACATCCGTCTGGTGACCAGTCGTACCGGCTGG GCACTGCCACCCTCACCCCACCCATACACCAATGAATGGCTCCAAGTGGACCTGGGAGATGAGAAGATAG TAAGAGGTGTCATCATTCAGGGTGGGAAGCACCGAGAAAACAAGGTGTTCATGAGGAAGTTCAAGATCGC CTATAGTAACAATGGCTCTGACTGGAAAACTATCATGGATGACAGCAAGCGCAAGGCTAAGTCGTTCGAA GGCAACAACAACTATGACACACCTGAGCTTCGGACGTTTTCACCTCTCTCCACAAGGTTCATCAGGATCT ACCCTGAGAGAGCCACACACAGTGGGCTTGGGCTGAGGATGGAGCTACTGGGCTGTGAAGTGGAAGCACC TACAGCTGGACCAACCACACCCAATGGGAACCCAGTGGATGAGTGTGACGACGACCAGGCCAACTGCCAC AGTGGCACAGGTGATGACTTCCAGCTCACAGGAGGCACCACTGTCCTGGCCACAGAGAAGCCAACCATTA TAGACAGCACCATCCAATCAGAGTTCCCGACATACGGTTTTAACTGCGAGTTTGGCTGGGGCTCTCACAA GACATTCTGCCACTGGGAGCATGACAGCCATGCACAGCTCAGGTGGAGTGTGCTGACCAGCAAGACAGGG CCGATTCAGGACCATACAGGAGATGGCAACTTCATCTATTCCCAAGCTGATGAAAATCAGAAAGGCAAAG TAGCCCGCCTGGTGAGCCCTGTGGTCTATTCCCAGAGCTCTGCCCACTGTATGACCTTCTGGTATCACAT GTCCGGCTCTCATGTGGGTACACTGAGGGTCAAACTACGCTACCAGAAGCCAGAGGAATATGATCAACTG GTCTGGATGGTGGTTGGGCACCAAGGAGACCACTGGAAAGAAGGACGTGTCTTGCTGCACAAATCTCTGA AACTATATCAGGTTATTTTTGAAGGTGAAATCGGAAAAGGAAACCTTGGTGGAATTGCTGTGGATGATAT CAGTATTAACAACCATATTTCTCAGGAAGACTGTGCAAAACCAACAGACCTAGATAAAAAGAACACAGAA ATTAAAATTGATGAAACAGGGAGCACTCCAGGATATGAAGGAGAAGGGGAAGGTGACAAGAACATCTCCA GGAAGCCAGGCAATGTGCTTAAGACCCTGGATCCCATCCTGATCACCATCATAGCCATGAGTGCCCTGGG AGTACTCCTGGGTGCAGTCTGTGGAGTTGTGCTGTACTGTGCCTGTTGGCACAATGGGATGTCAGAAAGG AACCTATCTGCCCTGGAGAACTATAACTTTGAACTTGTGGATGGTGTAAAGTTGAAAAAAGATAAACTGA ACCCACAGAGTAATTACTCAGAGGCGTGAAGGCACGGAGCTGGAGGGAACAAGGGAGGAGCACGGCAGGA GAACAGGTGGAGGCATGGGGACTCTGTTACTCTGCTTTCACTGTAAGCTGGGAAGGGCGGGGACTCTGTT ACTCCGCTTTCACTGTAAGCTCGGAAGGGCATCCACGATGCCATGCCAGGCTTTTCTCAGGAGCTTCAAT GAGCGTCACCTACAGACACAAGCAGGTGACTGCGGTAACAACAGGAATCATGTACAAGCCTGCTTTCTTC TCTTGGTTTCATTTGGGTAATCAGAAGCCATTTGAGACCAAGTGTGACTGACTTCATGGTTCATCCTACT AGCCCCCTTTTTTCCTCTCTTTCTCCTTACCCTGTGGTGGATTCTTCTCGGAAACTGCAAAATCCAAGAT GCTGGCACTAGGCGTTATTCAGTGGGCCCTTTTGATGGACATGTGACCTGTAGCCCAGTGCCCAGAGCAT ATTATCATAACCACATTTCAGGGGACACCAACGTCCATCCACCTTTGCATCGCTACCTGCAGCGAGCACA GGAAAAAGAAACGAAAAGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6409596 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6409596 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19467 same embryo
	EMAGE:19468 same embryo
	EMAGE:19469 same embryo
	EMAGE:19466 same embryo
	EMAGE:19465 same embryo
	EurExpress:euxassay_005158 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source