EMAGE mouse gene expression spatial database

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EMAGE:19465

Pkd2 polycystic kidney disease 2 ( MGI:1099818)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005161. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005161_01	euxassay_005161_02	euxassay_005161_03	euxassay_005161_04

euxassay_005161_05	euxassay_005161_06	euxassay_005161_07	euxassay_005161_08	euxassay_005161_09

euxassay_005161_10	euxassay_005161_11	euxassay_005161_12	euxassay_005161_13	euxassay_005161_14

euxassay_005161_15	euxassay_005161_16	euxassay_005161_17	euxassay_005161_18	euxassay_005161_19

euxassay_005161_20	euxassay_005161_21	euxassay_005161_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19465_wholemount_strong.wlz

19465_wholemount_moderate.wlz

19465_wholemount_weak.wlz

19465_wholemount_possible.wlz

19465_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19465_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8810
Entity Detected:	Pkd2, polycystic kidney disease 2 ( MGI:1099818)
Sequence:	sense strand is shown >T8810 GGATCCCTGGCCGCTGGGGCCTTTTCGACGCGCCTAAGTCCAGTCCAGGCGAGGCCAGGGCCCGGGAGGC GGGCCGAGGGGGCGGGCGGAGGCGGGGAGCAGCAGGCCGACTCCGGGAAGAAAAGAACATGGCTCCTGCG GCAGAGGGCGGCGGCACTCCCGGGCGCGCTGGGCGCCAGTGAGCGCCATGGTTAACTCCAGACGCGTGCA GCCGCAGCCGCCCGGGGACGCGGGACGCTCGCCCGCGCCGCGAGCGTCCGGACCCGGGCGCCTGGTGGCG GGAGGCGCCGGCCTAGCTGTCCCCGGCGGCCTCGGGGAGCAGCGGGGCCTGGAGATCGAGATGGAGCGCA TCCGGCAGGCGGCCGCTCGGGATCCCCCGGCCGGAGCCTCGGCCTCGCCGTCTCCTCCGCTTTCGTCCTG CTCCAGGCAAGCGTGGAGCCGCGACAACCCGGGCTTTGAGGCAGAGGAGGATGACGACGACGACGAGGTG GAAGGAGAAGAAGGAGGGATGGTGGTAGAGATGGATGTGGAGTGGCGCCCGGGCAGTCGGAGGTCGGCCT CCTCCTCGGCCGTGAGCTCGGTGGGCGCCCGCGGCCGAGGGCTCGGGAGCTACCGCGGCGCGGCTCACCT GAGCGGGAGGCGGCGCCGGCTAGAGGACCAGGGCGCGCAGTGTCCCAGCCCCGCGGGCGGCGGGGACCCG CTGCATCGCCACCTCCCGCTGGAGGGCCAGCCACCCCGAGTGGCCTGGGCAGAGAGGCTGGTGCGAGGGC TGCGAGGTCTCTGGGGAACAAGACTCATGGAAGAGAGCAACGCCAACCGAGAGAAGTACCTGAAAAGTGT GTTACGGGAGCTGGTCACTTACCTCTTTTTCCTCGTAGTCTTGTGCATCTTGACCTACGGCATGATGAGC TCCAATGTGTACTACTACACTCGGACACTGTCACAGCTATTCATAGACACCCCAGTGTCGAAAACAGAGA AAACCAACTTTAAAACTCTTTCTTCCATGGAGGACTTCTGGAAGTTCACCGAAGGCTCCTTCCTGGATGG GCTGTACTGGAAGGCACAGACCAGCAACCACACGCAAGCTGACAACCGAAGCTTTATCTTCTATGAGAAC CTGCTGCTAGGAGTGCCGCGTCTACGCCAACTCCGAGTCAGAAACGGATCCTGCTCCATCCCTCAGGACC TGCGAGATGAAATTAAAGAGTGCTATGACGTCTACTCCGTCAGCAGTGAGGACAGAGCTCCATTTGGACC GCGGAATGGAACTGCGTGGATGTACACAAGTGAGAAGGAGCTGAATGGGAGCAGTCACTGGGGGATCATT GCGTCGTACAGTGGAGCGGGTTACTACCTGGATCTGTCCAGAACCAGGGAGGAGACAGCAGCCCAGCTTG CTGGCCTCAGGAGGAACTTCTGGCTGGACCGGGGCACGCGGGCAGCTTTTATAGACTTCTCGGTGTATAA CGCAAACATTAACCTGTTCTGTGTGGTCAGGTTATTGGCGGAGTTCCCAGCAACGGGTGGCGTGGTACCC TCTTGGCAGTTTCAGCCTGTAAAACTGATCCGCTATGTCACAGCCTTTGATTTCTTCCTGGCAGCCTGTG AGATCATCTTTTGTTTCTTTATCATTTACTATGTGGTGGAAGAGATATTGGAAATTCGGATTCACAGACT GAGCTATTTCAGGAGTTTCTGGAATTGTCTGGATGTTGTGATTGTCGTGTTATCTGTAGTAGCTATGGTG ATTAACATTTACCGAATGTCAAATGCAGAGGGGCTGCTACAGTTTCTTGAAGATCAAAATTCTTTCCCCA ACTTTGAGCATGTGGCATACTGGCAAATACAGTTCAACAATATAAGTGCTGTCATGGTATTTTTGGTCTG GATTAAGCTCTTCAAATTCATCAATTTTAATAGGACCATGAGCCAGCTCTCCACAACCATGTCTCGATGT GCCAAAGACCTCTTCGGCTTCACCATAATGTTCTCCATCATCTTCTTGGCATACGCACAGCTGGCATACC TTGTCTTCGGCACCCAGGTCGATGACTTCAGCACTTTCCAAGAATGTATCTTCACCCAGTTCCGCATCAT TTTGGGTGATATCAACTTCGCAGAGATCGAGGAAGCTAACCGAGTTTTGGGGCCACTTTATTTTACTACA TTTGTGTTCTTTATGTTCTTCATTCTTTTGAATATGTTCCTGGCGATCATCAATGATTCGTACTCTGAAG TGAAATCCGATCTGGCCCAGCAGAAAGCAGAAATGGAACTCTCAGACCTTATCAGAAAGGGCTGCCAAAA AGCACTGGTCAAACTAAAACTGAAAAGAAACACTGTAGATGCCATCTCAGAGAGTCTCCGGCAAGGTGGT GGCAAACTGAACTTTGATGAGCTTCGGCAAGACCTGAAAGGGAAGGGCCATACAGATGCAGAGATTGAGG CCATATTCACTAAATATGACCAGGATGGCGACCAGGAACTGACCGAGCGTGAGCATCAACAGATGAGAGA TGACTTGGAGAAAGAGAGGGAGGACCTAGACTTGGAACACAGCTCTTTACCACGTCCGATGAGCAGCAGA AGTTTCCCCAGAAGCCTGGATGACTCCGAGGAGGAGGATGACGAAGACAGTGGCCATAGCTCCAGGAGGA GGGGAAGCATCTCCAGTGGGGTTTCCTATGAAGAGTTCCAAGTACTGGTGAGGCGCGTGGACCGCATGGA GCACTCCATTGGCAGCATCGTTTCCAAGATTGATGCCGTGATTGTCAAGCTGGAGATCATGGAGCGGGCC AAGCTGAAGAGACGAGAGGTGTTAGGACGGCTGCTGGATGGCGTGGCTGAGGATGCGCGACTGGGTCGGG ACAGTGAGATCCACAGGGAGCAGATGGAGCGCCTGGTGCGGGAAGAGCTGGAGCGCTGGGAATCGGATGA TGCAGCTTCGCAAACAGGTCATGGTGTAAGCACACAAGTGGGACTCGGTGGCCAGCCCCACCCCAGAAAC CCGCGCCCTCCTTCCTCCCAGTCTGCAGAGGGCCTGGAAGGTGGAGGTGGAAATGGAAGTGCCAACGTCC ATGCCTAATAATCCACACGTGTTTGTGTGTTCCTGAGACACCGAGAGGTGGCTGTCCAGTACGACAATGA TAAGTTCGGTATTTATATACCCCGGCTGCATTATGATGCTGGTCTTTGTGACCAATTGTTATAATTTTCT GCACTTTAATTTATTTTACGTAAACTTAAACCCGTGGTTCAAGGAAGGTTTGTCTTACTTTTCTCATGTG AGAAATCTAGGTGTAAATATTGAGTTCAGAAAGAAGAAAACTTTGAAAGTACGCTGATTTGCTGCCCTGC CCGTGTCTTCAGGTGACAGTGTTTGGAAGTTAGAGAGCCTTCACTAGTCTCTGAGTTCGTGTGGAGCCAC CACAGTCTGCTGCATTAGTCCAGTGCGAGATGCTAAGGAAAAACATGACATTTCATTCCACTGAGGTATA ACTCGGAGCTTACTGCATATCTTTCTTCTAAGGAAGGAGTAGTATTTCAGCAACCTTTCTGGGGTGCAGA GAAAGGATATGTATGTAGCCACTGTGCTAAAATGTGTGTGAACAGTTGAGATAGATGCTAAGCGCCATAC CAGTTTGTGGCACTAAGCATATCATTTAATTTTTGTGTTATTTAGCTTTTGTAGAATTCTCCTTTTTTTC CCTTTAGATATAAGTTCATATATTTTTCTACTTTAGTCCTGTGAAGTTTATCCCTAGTCACCTTCCTGGT AGTCTCCCCTCTGTTAAGGGTATAGTTAAGATTTCACATATACAAGGTTCTTTGGACATCAACAATTTCT TAACTGTAATAATGTGTACATTACTTAATAAAGACTTGACATAGTAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6409512 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6409512 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19467 same embryo
	EMAGE:19468 same embryo
	EMAGE:19469 same embryo
	EMAGE:19466 same embryo
	EMAGE:19464 same embryo
	EurExpress:euxassay_005161 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source