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EMAGE:19466

Mpp3 membrane protein, palmitoylated 3 (MAGUK p55 subfamily member 3) ( MGI:1328354)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19466 EMAGE:19466 EMAGE:19466 EMAGE:19466 EMAGE:19466
"Pseudo-wholemount" of euxassay_005156. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005156_01 euxassay_005156_02 euxassay_005156_03 euxassay_005156_04
EMAGE:19466 EMAGE:19466 EMAGE:19466 EMAGE:19466 EMAGE:19466
euxassay_005156_05 euxassay_005156_06 euxassay_005156_07 euxassay_005156_08 euxassay_005156_09
EMAGE:19466 EMAGE:19466 EMAGE:19466 EMAGE:19466 EMAGE:19466
euxassay_005156_10 euxassay_005156_11 euxassay_005156_12 euxassay_005156_13 euxassay_005156_14
EMAGE:19466 EMAGE:19466 EMAGE:19466 EMAGE:19466 EMAGE:19466
euxassay_005156_15 euxassay_005156_16 euxassay_005156_17 euxassay_005156_18 euxassay_005156_19
EMAGE:19466 EMAGE:19466 EMAGE:19466
euxassay_005156_20 euxassay_005156_21 euxassay_005156_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19466Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19466_wholemount_strong.wlz
19466_wholemount_moderate.wlz
19466_wholemount_weak.wlz
19466_wholemount_possible.wlz
19466_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19466_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
diaphragm
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 08 09 16 17 18 19 20 21 22
vertebral axis musculature
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
brain
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
facial vii ganglion
strong strong
regionalstrong expression: see section 02 03 04 05 17 18 19 20
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 05 06 07 17 18
trigeminal v ganglion
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 15 16 17 18 19 20 21
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 03 04 05 06 07 15 16 17 18 19
trigeminal v nerve
strong strong
regionalstrong expression: see section 07 14
spinal cord
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14 15 16 17 18
cervico-thoracic ganglion
strong strong
regionalstrong expression: see section 09 10 16 moderate expression: see section 15
cervical ganglion
strong strong
regionalstrong expression: see section 08 16 moderate expression: see section 15
thoracic ganglion
moderate moderate
regionalmoderate expression: see section 11 12
dorsal root ganglion
strong strong
regionalstrong expression: see section 07 08 09 10 12 13 14 15 16 17 18 19
retina
strong strong
regionalstrong expression: see section 01 19 20 21 moderate expression: see section 22
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 05 06 07 08 09 11 12 13 14 moderate expression: see section 04
nasal cavity respiratory epithelium
strong strong
regionalstrong expression: see section 09 10
tongue
weak weak
regionalweak expression: see section 06 07 08 09 10 11 12
stomach
weak weak
regionalweak expression: see section 01 02 03 04 06 07 08
midgut
weak weak
regionalweak expression: see section 08 09 10 11 12 13 15 16 17 18
metanephros
moderate moderate
spottedmoderate expression: see section 07 weak expression: see section 17 18
testis
moderate moderate
regionalmoderate expression: see section 04 05 06 07 weak expression: see section 17 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8619
Entity Detected:Mpp3, membrane protein, palmitoylated 3 (MAGUK p55 subfamily member 3) ( MGI:1328354)
Sequence:sense strand is shown

>T8619
AGGCTGGTGGCTCCCGCGCGGCTCCGGGTCACCGATCCCAGATCCTGGCGCGACGAGAGGTCATAAGCAG
ATCCTGGTGATGTGTGTTCATTACTGAGAGATTCCTCTCCCACCCACACAAACTGTAGGGACAGCTGGGG
ACCTGCTGGGCACAGGCATGCCAGTACTGTCTGAAGACTCGGGTTTGCATGAGACCCTGGCACTGCTCAC
CTCTCAGCTCAGGCCTGACTCCAACCATAGGGAGGAGATGGGCTTCCTGAGAGATGTCTTCAGTGAGAAG
AGCCTCAGTTATTTAATGAAGATTCATGAGAAACTCCGCTATTATGAGAGGCAGAGTCCAACCCCAGTCC
TCCACAGCGCCATGGCCCTTGCTGAGGATGTGATGGAGGAGCTGCAGGCTGCCTCTGTGCACAGTGATGA
GCGGGAGCTGCTGCAGCTGCTGTCCACCCCCCACCTCAGGGCTGTGCTCATGGTCCACGACACGGTTGCC
CAGAAGAACTTCGATCCTGTTCTGCCCCCTCTGCCCGATAACATCGATGAGGATTTTGAAGAGGAGTCGG
TGAAGATTGTTCGCTTGGTGAAGAATAAGGAGCCCCTGGGTGCCACCATCCGACGGGATGAGCATTCGGG
GGCTGTTGTGGTGGCCAGGATCATGCGAGGGGGTGCGGCAGACCGGAGCGGTCTGGTCCACGTTGGCGAT
GAGCTCCGAGAGGTGAATGGGATCGCCGTCCTGCACAAGCGTCCAGACGAGATCAGCCAAATTCTGGCCC
AATCCCAGGGATCCATTACCCTGAAGATCATCCCTGCCACCCAGGAGGAAGACCGCTTCAAAGACAGCAA
GGTATTCATGCGTGCCCTCTTCCACTATGACCCTCGGGAGGACCGTGCCATCCCCTGCCAGGAGGCTGGC
CTGCCCTTCCAGCGGAGGCAGGTCCTGGAGGTGGTGAGCCAAGATGACCCCACCTGGTGGCAGGCCAAGA
GAGTAGGCGACACCAACCTTCGAGCTGGCCTCATCCCATCTAAGCAGTTCCAGGAGAGGCGGCTGAGCTA
CCGGAGAACCACTGGCACCTTACCAAGCCCCCAGAACTTCAAAAAGCCCCCCTATGATCAGCCTTGTGAC
AAAGAGACCTGTGACTGTGATGGGTACTTTAAAGGACATTATGTGGCTGGTCTTCGGAGAAGCTTCCGGC
TGGGCTGCAGGGAGAGGCTGGGTGGCTCGCAGGAAGCAAAGGTCCCTACAGGAGCTGAGTCTCAGGTGCT
GCTGACGTACGAGGAAGTGGCCAGGTACCAACACCAGCCTGGGGAGCGGCCCCGCCTGGTGGTTCTCATT
GGGTCCCTGGGAGCCCACCTCCATGAGCTGAAGCAAAGGGTGGTGGCTGAGGACCCCCAGCAGTTTGCCG
TTGCAGTTCCACACACCACCAGGCCCCGGAAGAGCCACGAGAGAGACGGGGTGGAGTACCATTTTGTCTC
CAAGCAAGCGTTTGAGGCCGACGTACATCACAACAAGTTCCTGGAGCATGGAGAATATAAAGAGAATCTC
TACGGCACCAGCCTAGAGGCCATTCAGGCTGTTATGGCCAAAAACAAAGTGTGTTTGGTGGATGTAGAAC
CAGAAGCACTAAGACACCTGAGGACCCCTGAGTTTAAGCCCTATGTTATATTTGTGAAGCCTGCAATTCA
GGAGAGAAGGAAGACGCCGCCGGTGTCCCCAGACTCTGAGGACATAGCATCTTCACTTGATGAACAGCAG
CAAGAGATGGCCGCCTCTGCTGCCTTCATAGATCAGCACTATGGGCACCTGATAGACACTGTGCTCGTGA
GGCAGGACCTGCAGGAGGCCTGCAGCCAGCTCAGAGCTGTCATAGAGACGCTGAGCAAGGACACTTCCTG
GGTACCCATCAGCTGGGTGAGGTAACTTCACCCCATAGCATCTGGTCTGAAGGGACCTTGAAGACCCTCG
AGTCCAGCCTCCCTCATGTACCATCAAGGAATCTCAAGTGCAGAGAGGAACAGGACTTCCCACGAGCCAT
CGCCACCGAGCATCGGTGGGAAGCCTTGCTCAGTGTTGGATTTCTGTCTGCTGTTTGCACTGCAGCCCTT
CACGAGCGGAGCTGAAAGGGGCAGTCCACAGAATAGCGTGTTGTTCATTAAAGTCTCGCAGGCAAGTTGG
CCTTGATTCTTTGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5718318 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5718318 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19467 same embryo
 EMAGE:19468 same embryo
 EMAGE:19469 same embryo
 EMAGE:19464 same embryo
 EMAGE:19465 same embryo
 EurExpress:euxassay_005156 same experiment
 MGI:4826431 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS