EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19493

Tmem33 transmembrane protein 33 ( MGI:1915128)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004897. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004897_01	euxassay_004897_02	euxassay_004897_03	euxassay_004897_04

euxassay_004897_05	euxassay_004897_06	euxassay_004897_07	euxassay_004897_08	euxassay_004897_09

euxassay_004897_10	euxassay_004897_11	euxassay_004897_12	euxassay_004897_13	euxassay_004897_14

euxassay_004897_15	euxassay_004897_16	euxassay_004897_17	euxassay_004897_18	euxassay_004897_19

euxassay_004897_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19493_wholemount_strong.wlz

19493_wholemount_moderate.wlz

19493_wholemount_weak.wlz

19493_wholemount_possible.wlz

19493_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19493_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 03 04 05 13 14 15

vibrissa

moderate

regional

moderate expression: see section 03 04 17 weak expression: see section 05 06 16

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 04 05 15

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 15 16 17

vagus x ganglion

moderate

regional

moderate expression: see section 05 06 14

trigeminal v nerve

moderate

regional

moderate expression: see section 15

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 13 14 15 weak expression: see section 04

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 11 13 14 15 16

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 10 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6872
Entity Detected:	Tmem33, transmembrane protein 33 ( MGI:1915128)
Sequence:	sense strand is shown >T6872 GTCTTTCTAGAAAGGCAGCTTTGGTCTGGACCGAGCGGGGCAGCGTCCCGGGCTCCCGAGTGTCTCCCAT GGCGGATACGACCCCGAACGGCCCCCAAGGGGCGGGCGCTGTGCAATTCATGATGACCAATAAATTGGAC ACAGCAATGTGGCTTTCTCGCCTGTTCACAGTTTATTGCTCCGCTCTGTTCGTTCTGCCTCTTCTTGGGT TGCATGAAGCAGCGAGCTTTTACCAGCGTGCTTTGCTGGCCAATGCTCTGACCAGCGCTCTGAGGCTGCA TCAGAGATTACCTCACTTCCAGTTGAGCAGAGCGTTCCTGGCTCAGGCCTTGTTAGAGGACAGCTGCCAC TACCTGCTGTATTCACTCATCTTCGTCAACTCCTACCCTGTCACAATGAGTATTTTTCCAGTCTTGTTAT TTTCTTTGCTCCATGCTGCCACATACACTAAGAAGGTCCTGGACGCAAAGGGTTCAAATAGTTTACCTCT GTTGAGATCATTCTTGGATAAATTAAGTACTAACCAACAAAATATTCTGAAATTCATCGCTTGTAATGAA ATATTCTTGATGCCTGCTACAGTTTTTATGCTCTTTAGTGGCCAAGGAAGTTTGCTCCAGCCTTTTATAT ATTATAGATTTCTTACTCTTCGATATTCCTCTAGAAGAAATCCATATTGTCGGAACTTGTTTAATGAACT GAGGATTGTGGTTGAACACATTATTATGAAGCCCTCCTGCCCACTGTTTGTGAGAAGACTATGTCTCCAG AGCATCGCCTTTATCAGCAGACTGGCACCAACAGTTGCGTAGTTTAACATCTAGTTAAGCTGTGTTTAGA AGATTAGCATTATTAGCTGGTAATTCTGATAGGGGTCCCAAATTACAGGTCTAACACTGACCTCAGTCCA ATTTACATGATTTACATAAATGCATCTCAGTGCAAAAATAATAATTTTCCTGGCATGTTAAATCAAGCCT TTAACACTTTCTGAGAAAATTTTACTGTACATTACTGTTTGTGGTTATGTAAGTTTGTATTTAGTGATAA ATGTTACTAAATTGTTTTAGATGCTGCTGTGCCTACATCATGAAATACATTTATTTCTTTTAAAAAAAAA AAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3965864 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3965864 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19495 same embryo
	EMAGE:19494 same embryo
	EMAGE:19497 same embryo
	EMAGE:19498 same embryo
	EMAGE:19496 same embryo
	EurExpress:euxassay_004897 same experiment
	MGI:4828800 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source