EMAGE mouse gene expression spatial database

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EMAGE:19495

Gyk glycerol kinase ( MGI:106594)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004858. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004858_01	euxassay_004858_02	euxassay_004858_03	euxassay_004858_04

euxassay_004858_05	euxassay_004858_06	euxassay_004858_07	euxassay_004858_08	euxassay_004858_09

euxassay_004858_10	euxassay_004858_11	euxassay_004858_12	euxassay_004858_13	euxassay_004858_14

euxassay_004858_15	euxassay_004858_16	euxassay_004858_17	euxassay_004858_18	euxassay_004858_19

euxassay_004858_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19495_wholemount_strong.wlz

19495_wholemount_moderate.wlz

19495_wholemount_weak.wlz

19495_wholemount_possible.wlz

19495_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19495_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6577
Entity Detected:	Gyk, glycerol kinase ( MGI:106594)
Sequence:	sense strand is shown >T6577 CTTCAGCAGAAGCGCGCTGCTTTGCCCTCCGCTGTCCCTGCTCCGCCCTCCGCAGCCCTCCACAGTCACC CCGGAGACCAGCCGTGTTAAGCTCTCTGCTCTGAAGCTGACTGACTTCCATGGCAGCCGCGAAGAAAGCA GTTCTGGGGCCATTGGTGGGAGCAGTGGACCAGGGTACCAGCTCGACACGTTTTTTGGTTTTCAATTCAA AAACAGCTGAACTTCTTAGTCATCATCAAGTAGAAATAAAACAGGAATTCCCAAGAGAAGGATGGGTAGA ACAAGACCCGAAGGAAATTCTGCAGTCTGTTTATGAGTGTATAGAGAAAACGTGTGAGAAACTTGGACAG CTCAATATTGATATTTCCAACATCAAAGCCATTGGTGTCAGCAACCAGAGGGAAACCACAGTAGTCTGGG ACAAGGTCACCGGAGAGCCTCTCTATAATGCCGTGGTGTGGCTTGACCTAAGAACCCAGTCTACTGTTGA GAACCTTAGTAAAAGAATTCCAGGAAATAATAACTTTGTCAAGTCCAAGACAGGCCTTCCACTTAGCACG TATTTCAGTGCAGTGAAACTTCGTTGGCTCCTTGACAACGTGAAAAAGGTCCAAGAGGCTGTTGAAGAAA ATAGAGCTCTTTTTGGGACCATTGATTCATGGCTTATTTGGAGTTTAACAGGAGGAATCCATGGGGGTGT CCACTGTACAGATGTAACAAATGCAAGCAGGACGATGCTTTTTAACATTCATTCTTTGGAATGGGATAAA GAGCTCTGCGAATTTTTTGGAATTCCAATGGAAATTCTTCCCAACGTTCGGAGTTCTTCTGAGATCTATG GCCTAATGAAAGCTGGGGCCTTGGAAGGTGTACCAATATCTGGGTGTTTGGGGGACCAGTCTGCTGCTTT GGTGGGACAAATGTGCTTCCAGGATGGACAGGCCAAAAACACGTATGGAACAGGGTGCTTCTTATTGTGC AACACGGGCCATAAGTGTGTATTTTCTGAACATGGCCTCCTGACAACCGTAGCATATAAACTTGGCAGAG ACAAACCTGTGTATTATGCGTTGGAAGGTTCCGTGGCTATAGCTGGTGCTGTAATCCGCTGGCTAAGAGA CAACCTTGGAATTATTAAGTCCTCTGAGGAAATTGAAAAACTTGCTAAGGAAGTAGGTACTTCTTATGGC TGCTACTTCGTTCCAGCATTTTCAGGGTTATATGCGCCTTATTGGGAGCCCAGTGCAAGAGGGATCATCT GTGGACTCACTCAGTTCACCAATAAATGTCATATCGCTTTTGCTGCACTAGAAGCTGTTTGTTTCCAAAC CCGAGAGATTTTGGATGCCATGAATCGCGACTGTGGAATTCCACTCAGTCATTTACAGGTAGATGGAGGA ATGACCAGCAATAAAATTCTTATGCAGCTACAAGCAGACATTCTGTATATTCCAGTAGTGAAACCCTCCA TGCCTGAAACAACTGCACTAGGCGCTGCCATGGCAGCTGGGGCTGCAGAGGGGGTTGGTGTGTGGAGTCT TGAACCTGAGGATTTGTCAGCTGTCACAATGGAGCGGTTTGAACCTCAGATCAATGCTGAAGAAAGCGAA ATCCGTTACTCCACATGGAAGAAAGCTGTGATGAAGTCAATTGGTTGGGTTACAACTCAGTCTCCAGAAA GTGGTATCCCATAAATAATACCACCTCACGGATTTCCAAGATGCAAGCTTTTTAATGTGATATGAAAATC TGACTATTCTGTCTCATAGTATAATGATGCTATTCATAGACTCTGATTTTTTTCATAAGCCACTGGCTGC ATGATCCTCTAAGCAGACCTATGACTTGAAATAAAGAAAGTGCAGCAGAAAGAATCCTCCAGAAACATTT AATTTTTTTTTAACATTGACAGTTAAGATCGGGTCAGTCACCTTTGAGGCTGACCTCTGCCTCCACTGTC ATGATGTCCTACACTATTCCCGTTAAGGTCTAGGGTGATTTTGTATCCTGTCTATTGAAATGTGCCATTC AGTATATTCAGATGCTAGTGGATTACACATGTTTGAGGAAGAGGTTGTTACTAACCTGTTCAAAATGAGT GGCTTCTTGCTTGTTTGCTTTTAACAGCTCAGATGTCTTCTTTTCTATATATTAGAAGGCCACAACATTA CTGGATATTTCAAATGGAAACATCTAAAGAATTGTTGGATAATGATTTGCTATTCTTGTGGCTTAGACAT TTTCTGTACAGTGTTTGCCAAAATTCAACCTTGTCAGGTGTTTTACACTGTCCACTAACTACCATAGCTT TCTGTCTGGCTCTTACAGGATAGAACACTTTCTTTTTCTGCTTTTTTTTCATTTCTCCTTTTTATATTTT TATTCTGTATGTATAACATACATGCCTATATATTTTATATGCTGAGAGTAACCCATTTATAAATTAAGAG CACATTATATTCAATAAGTTATAAGAGGGCTGGTCTTAAGTGGACTACTATGTATATAAATTGGGGGAGG GCAAGCTGTATACATTTTTGGGCAACGGTTATGCATATTATTTACCAGAAGAAAATTTTCTTATAAAATC AAACATTTGAAACTTAAGTATGTATTCAGTCAATTAAAATGTACTATATTGTAATTTCAACTATATTTCT TATACTCTACCATTTAATTATCTCAGCTGGAAGAAAAGCAACTGTGGCATGTAACAGTAAATACTCTTCT CAAACATGATTCTAGTCCAAATAAAATTTTTCCTTTGAATTGATGTTTTCCATATTTGAGCTCCTATTGA AGAATTATCAAATTCAAAAGCTAAGGGGAAAAATATGTGTGAATAAAGCCAATAATGCCTTTTAAATGTT ATTACCCATTAATAAGATGTTCTTATTTTCTGGTATGATAGAAAATTATGTCTACTTAATAACTGACAAA AAAAAAACAAGTATCATTGCAAATGCCTCACAGTATAACCAGTAACTTCTCAAATATTTAGAAGGATTGG TTATAACTCATTAACCTAGCTTCCTAACATCAATTTTAAAATGTATTTGTTTTATAGTAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:3593896 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3593896 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19494 same embryo
	EMAGE:19497 same embryo
	EMAGE:19493 same embryo
	EMAGE:19498 same embryo
	EMAGE:19496 same embryo
	EurExpress:euxassay_004858 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source