EMAGE mouse gene expression spatial database

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EMAGE:19497

Klhl24 kelch-like 24 (Drosophila) ( MGI:1923035)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004876. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004876_01	euxassay_004876_02	euxassay_004876_03	euxassay_004876_04

euxassay_004876_05	euxassay_004876_06	euxassay_004876_07	euxassay_004876_08	euxassay_004876_09

euxassay_004876_10	euxassay_004876_11	euxassay_004876_12	euxassay_004876_13	euxassay_004876_14

euxassay_004876_15	euxassay_004876_16	euxassay_004876_17	euxassay_004876_18	euxassay_004876_19

euxassay_004876_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19497_wholemount_strong.wlz

19497_wholemount_moderate.wlz

19497_wholemount_weak.wlz

19497_wholemount_possible.wlz

19497_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19497_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

adenohypophysis

moderate

regional

moderate expression: see section 09 10 11 12 13 14

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 15

trigeminal v ganglion

strong

regional

strong expression: see section 17 18 moderate expression: see section 02 03 04 05 06 07 16

vagus x ganglion

moderate

regional

moderate expression: see section 05 15

spinal cord

strong

homogeneous

strong expression: see section 06 07 08 09 10 11 12 13 14

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 06 07 13

cervical ganglion

moderate

regional

moderate expression: see section 06 14

thoracic ganglion

moderate

regional

moderate expression: see section 10 11 12

dorsal root ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 13 14 15

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6870
Entity Detected:	Klhl24, kelch-like 24 (Drosophila) ( MGI:1923035)
Sequence:	sense strand is shown >T6870 CCACGCGTCCGGGAAGGAAAAGAATTCCCATGCCATCGAGCTGTCCTCTCAGCCTGCAGCAGCTACTTCA GAGCCATGTTCTGTAACGACCACAGGGAGAGCCGAGAAATGCTGGTTGAGATCAATGGCATTTTAGCTGA AGCTATGGAATGCTTTCTGCAGTATGTGTATACTGGGAAGGTGAAGATCACAACAGAGAATGTCCAGTAC CTCTTCGAAACCTCCAGCCTCTTTCAGATTAGTGTTCTCCGTGATGCTTGTGCCAAGTTCTTAGAGGAAC AGCTTGATCCTTGCAATTGCCTCGGAATCCAGCGCTTTGCCGACACCCACTCGCTCAAAACACTCTTCAC AAAATGCAAGACCTTTGCATTACAGACTTTTGAGGATGTGTCCCAGCATGAAGAATTTCTCGAGCTTGAC AAAGATGAACTTATTGATTATATCTGCAGTGATGAACTTGTTATTGGTAAAGAGGAGATGGTTTTTGAGG CCGTCATGCGCTGGGTCTACCGGGCGGTTGACCTGCGAAGACCACTGTTACATGAGCTCCTGACACACGT CAGGCTTCCTCTCTTGCATCCCAACTACTTTGTTCAGACAGTTGAAGTGGACCAGTTGATCCAGAATTCC CCCGAGTGTTATCAGTTGCTGCACGAAGCAAGACGATACCACATACTTGGCAATGAAATGATGTCCCCAA GGACTAGGCCACGCAGGTCTACTGGCTATTCAGAGGTGATAGTTGTGGTTGGAGGCTGTGAACGAGTTGG AGGATTTAATCTTCCATACACTGAGTGCTATGATCCAGTAACAGGAGAATGGAAGTCCTTGGCCAAGCTT CCAGAATTTACCAAATCCGAGTATGCAGTCTGTGCACTGAGGAATGATATTCTTGTTTCAGGTGGAAGAA TCAACAGCCGTGATGTCTGGATTTATAATTCACAGTTAAATATCTGGATCAGAGTTGCTTCTCTAAATAA AGGCAGATGGCGTCACAAAATGGCTGTCCTTCTTGGTAAAGTGTATGTTGTTGGCGGCTATGATGGGCAA AACAGGCTTAGCAGTGTGGAGTGTTACGATTCCTTTTCAAATCGTTGGACAGAAGTTGCTCCACTTAAGG AAGCAGTGAGCTCTCCTGCAGTGACAAGCTGTATAGGAAAATTGTTTGTGATTGGTGGAGGACCTGATGA TAATACTTGTTCTGATAAGGTCCAATCTTATGACCCAGAAACCAATTCTTGGCTACTTCGTGCAGCTATC CCTATCGCCAAGAGGTGCATCACAGCTGTCTCTTTAAACAACCTGATCTACGTAGCTGGTGGGCTGACCA AGGCAGTGTATTGTTACGATCCCGTTGAAGACTACTGGATGCATGTACAAAACACATTCAGCCGGCAGGA AAACTGTGGTATGTCTGTTTGTAATGGTAAAATCTATATCCTGGGTGGAAGACGGGAAAATGGAGAAGCC ACAGATACTATTCTCTGTTACGATCCTGCGACAAGTATCATCACAGGAGTGGCTGCAATGCCCAGGCCAG TGTCCTACCACGGCTGTGTAACTATTCATAGATACAATGAGAAGTGCTTTAAGCTCTAACTAAACCCAGG ATACCTCACACAAGAAGCCAACGCCAATCCAAGAGGAAAGTTTTTAAAACGACCACGTGAGAACTCAATT CCTTTGCATAAAGAAATAAATAATCCTGATGCCTTTCTGACCCAAAATTCCAATATTTCAAGCAGTCATA AATCCTTTCCTGTACTGGGGGTGGGAGGTTGGTTGAGTGAGACCAGTGCTCACCTTTACCCCAGCACTCA GAAGGCTTCTATACTTCTATACGTTTGAACCCAGCCTGGTCTACATAACAAGTTCTAGGCCAGCAAAGGT TACACAATAAGGCCTTGTCTTTTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3965863 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3965863 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19495 same embryo
	EMAGE:19494 same embryo
	EMAGE:19493 same embryo
	EMAGE:19498 same embryo
	EMAGE:19496 same embryo
	EurExpress:euxassay_004876 same experiment
	MGI:4825798 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source