EMAGE mouse gene expression spatial database

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EMAGE:19534

Tram2 translocating chain-associating membrane protein 2 ( MGI:1924817)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004974. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004974_01	euxassay_004974_02	euxassay_004974_03	euxassay_004974_04

euxassay_004974_05	euxassay_004974_06	euxassay_004974_07	euxassay_004974_08	euxassay_004974_09

euxassay_004974_10	euxassay_004974_11	euxassay_004974_12	euxassay_004974_13	euxassay_004974_14

euxassay_004974_15	euxassay_004974_16	euxassay_004974_17	euxassay_004974_18	euxassay_004974_19

euxassay_004974_20	euxassay_004974_21	euxassay_004974_22	euxassay_004974_23	euxassay_004974_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19534_wholemount_strong.wlz

19534_wholemount_moderate.wlz

19534_wholemount_weak.wlz

19534_wholemount_possible.wlz

19534_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19534_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 09 10 11 14 15 16 17

naris

moderate

regional

moderate expression: see section 10 11 12 13 14 15

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09

viscerocranium

moderate

regional

Expression in the turbinate bone.

hyoid

moderate

regional

moderate expression: see section 10 11 12 13 14

axial skeleton

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17

temporal bone petrous part

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 16 17 18 19 20 21 22 23 24

sternum

moderate

regional

moderate expression: see section 12 13

axial skeleton tail region

moderate

regional

moderate expression: see section 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6916
Entity Detected:	Tram2, translocating chain-associating membrane protein 2 ( MGI:1924817)
Sequence:	sense strand is shown >T6916 CGGACGCGTGGGTGCGGACCGGGCCGGGCCGGAGAAGTTTTCGCTGGGGCTGCTGAGCGAGGGTGCGGGC CCCCGCAGGCGGTGAACGCCCGCTGCCATGGCTTTCCGTAGGAGGACGAAAAGTTACCCGCTCTTCAGCC AGGAGTTCATCATCCACAACCATGCAGACATCGGTTTCTGCTTGGTGCTCTGCGTCCTCATCGGGCTTAT GTTCGAGGTCACAGCCAAGACTGCCTTCCTATTTATTTTACCTCAGTATAACATTAGTGTGCCTACAGCA GACAGTGAGACAGTGCACTACCACTATGGCCCAAAGGACCTGGTCACCATCTTGTTCTACGTCGTCATCA CCATCATCTTCCATGCTGTGGTCCAGGAGTACATCTTAGACAAAATCAGCAAACGCCTTCATCTCTCCAA GGTCAAACACAGCAAGTTCAATGAATCTGGACAGCTGCTTGTCTTTCATCTCAGCGCGGTGGCGTGGTGC TTCTATGTGATCGTAACAGAAGGATATTTAACAAACCCAAGAAGCCTCTGGGAAGATTACCCGCATGTGT ACCTCTCCTTCCAGGTGAAGTTTTTCTACTTGGGTCAGCTGGCATACTGGTTGCACTCACTACCTGAACT CTACTTCCAGAAGGTGCGGAAGGAAGAAGTCCCCCGCCAGCTGCAATACATTTGTCTGTACCTTCTTCAC ATCACTGGGGCATATCTCTTAAACCTGAGCCGCCTGGGGCTGATCTTGCTACTACTGCAGTACTCCACCG AGGCCCTCTTTCACATGGCTCGGCTCTTCCACTTTGCAGACGAGAATAACGAGAGGCTGTTTAACGCCTG GGCTGCTGTGTTTGGGGTCACGCGTCTCTTCATCCTCACCCTCGCTGTGCTGACCATTGGCTTTGGATTG GCTCGGGTGGAAAACCAAGTGTTTGACCCCGAGAAAGGGAACTTTAACACCTTGCCTTGTAGGCTTGGCA TGCTGCTGCTGGTGTGTGTCGCCCAGGCCTGGCTCATGTGGCGCTTCATCCACTCCCAGTTGCGGCACTG GCGGGAGTATTGGAAGGAGCAGAGTGCCAAGCGTAGAGTCTCTGCTGTCCCCAGGCCCCCAGCCAAGCTC CTCAAGAGAGAACCTGGTTACCATGAAAACGGAGTGGTGAAGGCAGAGAACGGGACCTCCTCACGGACTA AGAAACTCAAGTCTCCCTAGCGCCACTTTGCTGAGAACAGGGATCCTCTGGATGAGGAAGCCCTGGCCCC AGCTCAGTCTCTCCACCCTCCTGCCCATGCTCGTCTCCACCAGCAGAAGCCTTGGCATCAAAGGGGGAGT TCCTCTCTACCTTCTAGTCTTTCTCTTCTTTCATAAACCATTCTCAATAAAGCCAAAAATCTTAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3980004 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3980004 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19535 same embryo
	EMAGE:19532 same embryo
	EMAGE:19537 same embryo
	EMAGE:19536 same embryo
	EMAGE:19533 same embryo
	EurExpress:euxassay_004974 same experiment
	MGI:4828892 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source