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EMAGE:1956

Tbx2 T-box 2 ( MGI:98494)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:1956 EMAGE:1956

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
The data from image 1 (left hand view) has been spatially mapped in this EMAGE entry. See EMAGE:1957 for the spatially data from the right hand view (image 2). Image 1 shows the left hand side of the embryo, this view has been spatially mapped. Image 2 shows the right hand side of the embryo.
Expression Pattern Description
Spatial Annotation:
EMAGE:1956Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
1956_wholemount_strong_3D_1.wlz
1956_wholemount_moderate_3D_1.wlz
1956_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1956_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
head mesenchyme
detected detected
regionalexpression is detected in the facial mesenchyme
optic cup
detected detected
expression is detected in the dorsal retina
otic pit
detected detected
expression is detected in the otic vesicle
atrioventricular canal
detected detected
forelimb bud
detected detected
trunk somite
detected detected
regional
genitourinary system
detected detected
regionalexpression is detected in the nephrogenic mesoderm.
Annotation Validation: submitter
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3531302
Entity Detected:Tbx2, T-box 2 ( MGI:98494)
Notes:The Tbx2 probe used in this study by Harrelson is described as follows, "The probe contains ~2kb of Tbx2 cDNA (full-length cDNA is ~2.3kb). An alkaline phosphatase-conjugated anti-digoxigenin antibody was used to develop the color from BM purple AP substrate (both from Roche)."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Zach Harrelson, Virginia Papaioannou.
Principal investigator:Virginia Papaioannou, Columbia University, 701 W. 168th St. Dept of Genetics & Development HHSC 1402, New York, USA NY 10032
Submitted by:Zach Harrelson, Columbia University, 701 W. 168th St. Dept of Genetics & Development HHSC 1402, New York, USA NY 10032
Experiment type:non-screen
References:[ doi:10.1242/dev.01378] [ PMID:15459098] Harrelson Z, Kelly RG, Goldin SN, Gibson-Brown JJ, Bollag RJ, Silver L 2004 Tbx2 is essential for patterning the atrioventricular canal and for morphogenesis of the outflow tract during heart development. Development (131):5041-52
Links:EMAGE:1957 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE