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EMAGE:19567

Mrpl32 mitochondrial ribosomal protein L32 ( MGI:2137226)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19567 EMAGE:19567 EMAGE:19567 EMAGE:19567 EMAGE:19567
"Pseudo-wholemount" of euxassay_004986. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004986_01 euxassay_004986_02 euxassay_004986_03 euxassay_004986_04
EMAGE:19567 EMAGE:19567 EMAGE:19567 EMAGE:19567 EMAGE:19567
euxassay_004986_05 euxassay_004986_06 euxassay_004986_07 euxassay_004986_08 euxassay_004986_09
EMAGE:19567 EMAGE:19567 EMAGE:19567 EMAGE:19567 EMAGE:19567
euxassay_004986_10 euxassay_004986_11 euxassay_004986_12 euxassay_004986_13 euxassay_004986_14
EMAGE:19567 EMAGE:19567 EMAGE:19567 EMAGE:19567 EMAGE:19567
euxassay_004986_15 euxassay_004986_16 euxassay_004986_17 euxassay_004986_18 euxassay_004986_19
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euxassay_004986_20 euxassay_004986_21 euxassay_004986_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19567Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19567_wholemount_strong.wlz
19567_wholemount_moderate.wlz
19567_wholemount_weak.wlz
19567_wholemount_possible.wlz
19567_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19567_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8036
Entity Detected:Mrpl32, mitochondrial ribosomal protein L32 ( MGI:2137226)
Sequence:sense strand is shown

>T8036
GGGCGGACTTAACGCATGCGCTCAGCCCGCGTCCCGCTCTGTCTTGAGAAGATCATGGCTCCTTCGTTGC
TGCTGCTTTCACTACCATGGCCGGTGCGTCCCGGACCGCTCCAGAGGTGCTGGGAGCTGCTACAACGGCA
ACTGCAGCAGAGCTGGAGTCGCTTTGTCAGTCCTCCGTGGGCACCAGCATTAGCCGTCCAGAGACCATCC
ATCCTCACAGAGCTAGCACATGATACTTGTGAAAATAAAGAGAATTCCAGCTTTTTAGATAGTATCTTTT
GGATGGCAGCTCCCAAAAACAGACGCACCATCGAAGTTAACCGATGTAGGAGAAGAAACCCTCAAAAGCT
TATTAAAATTAAGAACAATATAGACATTTGCCCTGAATGTGGTCACCTGAAGCAGAAACATGTCCTTTGT
GGATATTGCTATGAGAAAGTACGCCAGGAGACGACAAAAATCAGACAACAAATAGGGGCTCAAGAAGGAG
GTCCTTTCAGAGCTCCTTCCGTTGAGACTATGGTGCTGTACACAGGAGAGAAACCATCAGAAAAGGATCA
GGGCAAGAGGATTGTTGAAAGAAACATAAAGAGGCCATCTTGGTTCACCCAGAATTGAACTAAAAAATGT
TAAAAAGATAATTTCATAGTTAGGCACTTATTTAAAATCAGGAGTCTTCATTTTCGTGTTGTGCCTGTTT
TCAAATTATCAATGTATTTTAAAATGGATTTTGTTAGTTGTTACTTTTAAATGAGGTAATTTGAAGACTG
TATCATGAATAAACTCAGCATATTTATGTAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:30018135 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30018135 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19568 same embryo
 EMAGE:19571 same embryo
 EMAGE:19570 same embryo
 EMAGE:19569 same embryo
 EMAGE:19572 same embryo
 EurExpress:euxassay_004986 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS