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EMAGE:1958

Tbx2 T-box 2 ( MGI:98494)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:1958 EMAGE:1958

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
The vibratome section shown was taken from the wholemount stained embryo at the approximate plane indicated by the red line.
Expression Pattern Description
Spatial Annotation:
EMAGE:1958EMAGE:1958Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
1958_voxel_strong_3D_1.wlz
1958_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1958_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
genitourinary system
detected detected
expression is detected in the nephrogenic mesoderm.
Annotation Validation: submitter
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3531302
Entity Detected:Tbx2, T-box 2 ( MGI:98494)
Notes:The Tbx2 probe used in this study by Harrelson is described as follows, "The probe contains ~2kb of Tbx2 cDNA (full-length cDNA is ~2.3kb). An alkaline phosphatase-conjugated anti-digoxigenin antibody was used to develop the color from BM purple AP substrate (both from Roche)."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:sectioned wholemount
Procedures
Fixation:4% paraformaldehyde
Embedding:0.44% gelatin, 14% BSA, 18% sucrose in PBS
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Zach Harrelson, Virginia Papaioannou.
Principal investigator:Virginia Papaioannou, Columbia University, 701 W. 168th St. Dept of Genetics & Development HHSC 1402, New York, USA NY 10032
Submitted by:Zach Harrelson, Columbia University, 701 W. 168th St. Dept of Genetics & Development HHSC 1402, New York, USA NY 10032
Experiment type:non-screen
References:[ doi:10.1242/dev.01378] [ PMID:15459098] Harrelson Z, Kelly RG, Goldin SN, Gibson-Brown JJ, Bollag RJ, Silver L 2004 Tbx2 is essential for patterning the atrioventricular canal and for morphogenesis of the outflow tract during heart development. Development (131):5041-52
Links:EMAGE:1956 same embryo
 EMAGE:1957 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE