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EMAGE:1959

Tbx2 T-box 2 ( MGI:98494)
TS12 (8.5 dpc)
in situ hybridisation

Data Images
EMAGE:1959 EMAGE:1959

Expression pattern clarity: no stars
Notes:
The vibratome section shown was taken from the wholemount stained embryo at the approximate plane indicated by the red line. Image 1 shows a vibratome section taken from the wholemount shown in Image 2.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
heart
detected detected
expression is detected in the cardiac mesoderm and cardiac crescent.
Annotation Validation: submitter
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3531302
Entity Detected:Tbx2, T-box 2 ( MGI:98494)
Notes:The Tbx2 probe used in this study by Harrelson is described as follows, "The probe contains ~2kb of Tbx2 cDNA (full-length cDNA is ~2.3kb). An alkaline phosphatase-conjugated anti-digoxigenin antibody was used to develop the color from BM purple AP substrate (both from Roche)."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:8.5 dpc
Theiler Stage:TS12
Mutations:none (wild-type)
Preparation:sectioned wholemount
Procedures
Fixation:4% paraformaldehyde
Embedding:0.44% gelatin, 14% BSA, 18% sucrose in PBS
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Zach Harrelson, Virginia Papaioannou.
Principal investigator:Virginia Papaioannou, Columbia University, 701 W. 168th St. Dept of Genetics & Development HHSC 1402, New York, USA NY 10032
Submitted by:Zach Harrelson, Columbia University, 701 W. 168th St. Dept of Genetics & Development HHSC 1402, New York, USA NY 10032
Experiment type:non-screen
References:[ doi:10.1242/dev.01378] [ PMID:15459098] Harrelson Z, Kelly RG, Goldin SN, Gibson-Brown JJ, Bollag RJ, Silver L 2004 Tbx2 is essential for patterning the atrioventricular canal and for morphogenesis of the outflow tract during heart development. Development (131):5041-52
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE