EMAGE mouse gene expression spatial database

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EMAGE:19607

Abhd3 abhydrolase domain containing 3 ( MGI:2147183)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005011. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005011_01	euxassay_005011_02	euxassay_005011_03	euxassay_005011_04

euxassay_005011_05	euxassay_005011_06	euxassay_005011_07	euxassay_005011_08	euxassay_005011_09

euxassay_005011_10	euxassay_005011_11	euxassay_005011_12	euxassay_005011_13	euxassay_005011_14

euxassay_005011_15	euxassay_005011_16	euxassay_005011_17	euxassay_005011_18	euxassay_005011_19

euxassay_005011_20	euxassay_005011_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19607_wholemount_strong.wlz

19607_wholemount_moderate.wlz

19607_wholemount_weak.wlz

19607_wholemount_possible.wlz

19607_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19607_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lateral ventricle choroid plexus

strong

regional

strong expression: see section 05 06 07 15 16 moderate expression: see section 08 09 14 weak expression: see section 10 11 12

4th ventricle lateral recess

moderate

regional

moderate expression: see section 13 14 weak expression: see section 01 02 03 04 10 11 12 15 16

rest of cerebellum mantle layer

strong

regional

strong expression: see section 12 13 14 moderate expression: see section 01 02 03 04 11 15 16

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 10 11 12 13 14 15 16

urethra of male

moderate

regional

moderate expression: see section 10 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7441
Entity Detected:	Abhd3, abhydrolase domain containing 3 ( MGI:2147183)
Sequence:	sense strand is shown >T7441 GCACTATGCAGCGCCTGGCCATGGACCTGCGGGTGCTGTCCCGGGAGCTGGCGCTCTACCTGGAGCACCA GGTTCGCGTCGGCTTCTTCGGCTCGGGCGTGGGCTTGTCACTGATCTTGGGATTCAGCGTTGCCTATGCT TGCTACTACCTGAGTAGCATTGCCAAGAAACCCCAGTTAGTGATTGGAGGGGAGAGTTTCAGCCGCTTCC TACAGGACCACTGTCCCGTGGTGACAGAAACCTATTACCCGACGGTCTGGTGCTGGGAGAGCCGAGGACA GACATTGCTGAGACCCTTCATCACTTCCAAACCCCCGGTGCAATACAGGAACGAACTCATTAAAACTGCA GACGGAGGACAGATCTCACTGGACTGGTTTGATAACAATAACAGTGCGTACTATGTGGATGCCAGCACCA GACCTACTATCTTGCTCTTGCCTGGCCTCACTGGAACAAGCAAGGAATCCTACATCCTTCATATGATCCA TCTCAGTGAAGAATTAGGATACAGGTGTGTGGTTTTTAATAACAGAGGAGTAGCAGGAGAAAGTCTCTTG ACACCACGGACTTACTGCTGTGCGAACACTGAAGACTTGGAGGCCGTCGTCCACCACGTGCACAGCCTGT ACCCTGGGGCTCCTTTTCTGGCAGCAGGCGTATCAATGGGAGGAATGCTGCTTCTGAATTACTTGGGTAA AATTGGGTCCAAAACCCCTCTGATGGCTGCTGCAACGTTTTCGGTTGGCTGGAACACTTTCGCTTGCTCA GAGTCTCTGGAGAGGCCGCTGAACTGGCTACTTTTTAATTACTACTTAACAACTTGCCTCCAGTCATCAG TTAAGAAGCACCGGCATATGTTTGTGGAGCAGATTGACATGGATCAGGTCATGAAGGCCAAGTCGATCAG AGAGTTTGATAAGCGGTTCACGGCGGTCATGTTTGGGTACCGCACACTCGATGATTACTACACCGATGCC AGTCCAAACCGCAGGCTGAAGTCAGTGGGAATCCCTGTTCTGTGCCTGAATGCTACGGACGACGTGTTCT CACCCAGCCATGCTATCCCAATAGAAACTGCTAAACAAAACCCTAACGTGGCTTTGGTCCTTACAGCCTA TGGAGGCCATATTGGTTTTCTGGAGGGAATCTGGCCTAGGCAATGCACTTACATGGATCGAGTCTTCAAG CAATTTGTACAGGCCATGGTTGAGCACGGACATGAACTCTCCAACATGTAGCTTCTTGGCTGCATTCAGC CTGAGCCACTGTTGTTAAAGCAGCTCAGCTTGGAGCACAGGTTTCAACAGCTGAGGGTAGAGCTGACAAG CCGGCAGATGGGTGAAGAGGTCCAGCATGACATGCAAAACTACTTCTTAGGGAAACACAACAACTTTGTA GTAAGAAACTAAAGATAGTTTAGATTGTTACTGTAGTAGATCTTATTTTTCCTATGCCTTACCAAATATG ACCTTAAAATTAAGTAGTCCTTATGTAACAGAATTGCACTTAACCAAAACTATTGTGTAAAACGATTTTA ATTCCTCAGGGTTTTAATTTAGGCTAGTATTTTTAATTACTCAGTTTAGGTGACTTAATAATACTTTAAT AACTGTTAAGAGATTTTAATGATTTGAATATAAGTTAGAAGAGGGGTATTCATGGTAACAAGAAACTGAA AAGGATAAATTATATTGTTCTAAGTGTTTCTTCTGATTTAAAAGGCAAATGCAACAATGTTAGACTGACT TCTATATTTGCTCTCTTCTCTGATAATATTAAATTAGGGCTGATTTATGTTTTATAATTGTTATCATTTA CATGCTTATTTTTAAAATAGTATTTGTGAATACATATATCATATTAAAATAAATTCTATCATTTTAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4234600 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4234600 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19606 same embryo
	EurExpress:euxassay_005011 same experiment
	MGI:4822879 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source