EMAGE mouse gene expression spatial database

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EMAGE:19609

Bhmt2 betaine-homocysteine methyltransferase 2 ( MGI:1891379)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005041. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005041_01	euxassay_005041_02	euxassay_005041_03	euxassay_005041_04

euxassay_005041_05	euxassay_005041_06	euxassay_005041_07	euxassay_005041_08	euxassay_005041_09

euxassay_005041_10	euxassay_005041_11	euxassay_005041_12	euxassay_005041_13	euxassay_005041_14

euxassay_005041_15	euxassay_005041_16	euxassay_005041_17	euxassay_005041_18	euxassay_005041_19

euxassay_005041_20	euxassay_005041_21	euxassay_005041_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19609_wholemount_strong.wlz

19609_wholemount_moderate.wlz

19609_wholemount_weak.wlz

19609_wholemount_possible.wlz

19609_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19609_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver left lobe

strong

regional

strong expression: see section 01 02 03 04 05 09 10 11 moderate expression: see section 06 07 08

liver right lobe

strong

regional

strong expression: see section 12 13 14 15 16 17 18 19 20 not examined expression: see section 09

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7460
Entity Detected:	Bhmt2, betaine-homocysteine methyltransferase 2 ( MGI:1891379)
Sequence:	sense strand is shown >T7460 GGGACCCGCGCCTGTACAGCGGCGGCGACATGGCACCAGCTGGAAGCACTCGAGCCAAGAAGGGCATCTT GGAGCGTCTGGACAGCGGGGAGGTGGTGGTTGGGGACGGCGGCTTTCTCTTCACTCTGGAAAAGAGAGGC TTCGTGAAGGCGGGGCTTTGGACTCCAGAAGCAGTGGTAGAACATCCAAGTGCAGTTCGTCAACTTCACA CAGAATTCTTGAGAGCGGGAGCTGATGTCTTGCAGACATTCACCTTTTCTGCTACTGAAGACAATATGGC CAGCAAGTGGGAAGCTGTGAATGCAGCTGCCTGTGACCTGGCCCAGGAGGTGGCTGGTGGAGGGGGTGCT TTGGTGGCAGGGGGCATCTGCCAGACATCACTCTACAAGTACCACAAGGATGAAACTAGAATTAAAAACA TTTTTCGACTACAGCTAGAAGTTTTTGCCAGGAAAAATGTGGACTTCTTGATTGCAGAGTATTTTGAGCA TGTGGAAGAAGCTGTATGGGCTGTCGAAGTCTTGAGAGAGGTGGGGGCACCTGTGGCTGTGACCATGTGC ATCGGCCCAGAGGGGGACATGCACGACGTGACACCTGGAGAGTGTGCGGTGAAGCTGGCGCGTGCAGGGG CGGACATCATTGGGGTCAACTGCCGGTTTGGGCCCTGGACTAGCTTGCAGACCATGAAGCTCATGAAAGA GGGCCTCAGAGATGCCAGCCTGCAGGCTCACCTTATGGTCCAGTGCTTGGGCTTCCACACACCGGACTGT GGCAAGGGAGGGTTTGTGGACCTCCCAGAGTATCCCTTTGGCCTGGAGCCAAGAGTTGCCACCAGATGGG ATATTCAAAAATACGCCAGAGAGGCCTACAACCTGGGGATCAGGTACATTGGCGGCTGCTGCGGATTTGA GCCCTACCACATCAGGGCGATTGCAGAGGAACTGGCCCCAGAAAGGGGATTTTTGCCACCGGCTTCAGAG AAACATGGCAGCTGGGGAAGTGGTCTGAACATGCACACCAAACCCTGGATCAGAGCAAGGGCTAGACGAG AATACTGGGAGAATCTGCTGCCAGCCTCAGGAAGACCTTTCTGTCCTTCCCTATCAAAGCCAGATGCATA AGAAGCAATGAAAGAGAACTCTGAAGTGACAAGACAGGAGGAATTAGCCACAAGCCCCACCTGGAACCTT TCTTGCTGCTGTCTTCAGCCTACCCTGCTCTTCTGGCTGCTGAGCATCTATGAGTGGTATCCCTTCAGTT GTAGTGACATGTCACTCCTGAGCATGCTCATCAGATGCAGTGGAGATGCAGAGGCACCTGGACCCCACCC CACGCCCCGCTCACTCTCTGCCGAGTAATGCCACGGGCCTCCATTTGAAGGTCATTGGGCATGGCCAGAG TTGGCTCTCAGGGTGGATTTGACATTCTCAAATAATCCCAAGTCAGCAAGAAGCAACCTTGGTGAATAAT TCTGGTGTTGAAGCTAGGTCTTGAGCTACAGTCTCAGGAGAAGAGAGGCCAAAGCTGTTGTGAGTGATGG CAAGGCTATTGAACAGTCATGATGCTTGGCTTTCCAGGATGACTTCTCGCTTTAAAGTCCTGGAATTTGT AAGTGCATCTTTATAGCCATGCATGGCTAGATTATAAACTACTGATGGCCAAGGACCGTATAATATGCTT CTTACATTCCTCTCTGCTTAGCTTTATGCCAAACTTAATCACCAATGCTCAGGGAAACACTTGCTGATTA AAGCAAAACACATGAACCTTGCAAGGCCGTTTTAGAACTCAGAGAGCAAGTTCATCTTGTTCTAGCACTT TCTATGTTCATGTTTGTTTTGTACACTGTGCCCCACTTTTCTGTTTTTCACTTCAGTGTAGCAGTGTGTA ACAATGAGTAAGATTAAAGATAATTTTTCTTTTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4235455 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4235455 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19613 same embryo
	EMAGE:19611 same embryo
	EMAGE:19608 same embryo
	EMAGE:19610 same embryo
	EMAGE:19612 same embryo
	EurExpress:euxassay_005041 same experiment
	MGI:4823471 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source