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EMAGE:1962

Tbx2 T-box 2 ( MGI:98494)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:1962 EMAGE:1962 EMAGE:1962
Image 1. Anterior view of a dissected heart. Image 2. This shows the same anterior view of the same sample as in image 1, but the heart has been dissected to remove the outflow tract to show clearly the atrio-ventricular expression. Image 3. Posterior view of the dissected heart.

Expression pattern clarity: no stars
Notes:
All images are of the one sample. Image 1 - anterior view of the dissected heart. Image 2 - shows the same anterior view of the heart on image 1, but the sample has been dissected to remove the outflow tract to reveal atrio-ventricular expression. Image 3 - posterior view of the dissected heart. Image 1 shows the anterior view of a dissected heart of a TS15 embryo. Image 2 shows the anterior view of a dissected heart of a TS15 embryo with the outflow tract removed. Image 3 shows the posterior view of a dissected heart of a TS15 embryo.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
atrioventricular canal
detected detected
This is the domain of intense expression seen on the right of Image 2 and on the left of Image 3.
outflow tract
detected detected
Expression in the outflow tract is seen in Image 1 (Note that the outflow tract has been removed from the sample in Image 2).
Annotation Validation: submitter
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3531302
Entity Detected:Tbx2, T-box 2 ( MGI:98494)
Notes:The Tbx2 probe used in this study by Harrelson is described as follows, "The probe contains ~2kb of Tbx2 cDNA (full-length cDNA is ~2.3kb). An alkaline phosphatase-conjugated anti-digoxigenin antibody was used to develop the color from BM purple AP substrate (both from Roche)."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Zach Harrelson, Virginia Papaioannou.
Principal investigator:Virginia Papaioannou, Columbia University, 701 W. 168th St. Dept of Genetics & Development HHSC 1402, New York, USA NY 10032
Submitted by:Zach Harrelson, Columbia University, 701 W. 168th St. Dept of Genetics & Development HHSC 1402, New York, USA NY 10032
Experiment type:non-screen
References:[ doi:10.1242/dev.01378] [ PMID:15459098] Harrelson Z, Kelly RG, Goldin SN, Gibson-Brown JJ, Bollag RJ, Silver L 2004 Tbx2 is essential for patterning the atrioventricular canal and for morphogenesis of the outflow tract during heart development. Development (131):5041-52
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE