EMAGE mouse gene expression spatial database

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EMAGE:19621

Abcb8 ATP-binding cassette, sub-family B (MDR/TAP), member 8 ( MGI:1351667)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005065. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005065_01	euxassay_005065_02	euxassay_005065_03	euxassay_005065_04

euxassay_005065_05	euxassay_005065_06	euxassay_005065_07	euxassay_005065_08	euxassay_005065_09

euxassay_005065_10	euxassay_005065_11	euxassay_005065_12	euxassay_005065_13	euxassay_005065_14

euxassay_005065_15	euxassay_005065_16	euxassay_005065_17	euxassay_005065_18	euxassay_005065_19

euxassay_005065_20	euxassay_005065_21	euxassay_005065_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19621_wholemount_strong.wlz

19621_wholemount_moderate.wlz

19621_wholemount_weak.wlz

19621_wholemount_possible.wlz

19621_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19621_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7500
Entity Detected:	Abcb8, ATP-binding cassette, sub-family B (MDR/TAP), member 8 ( MGI:1351667)
Sequence:	sense strand is shown >T7500 AAGGATGGGAGCATAGAGATTGTCTGGAACAACTCTAGAGAAAGGGCTCAAGGTCAGAAGCGCCAGGACG TCCTCTAATGTGAGAATGAACGTCGCACAACCTCTGACCCGCACCTGACTTCAGGCCACTGAGCAAATAG CTAGAGCTGCTTCTGCTCGGTCCCGCCTACAGCCAACATTTTATCCAATCACAGCTTGCAGCAAGACTGA TGTGTCTTTTCTCCAATCATCGCTGAGAATGGGAAGGGGTTGTCGGGATGGGGCCATAGCGAACATGGAA CCCTCGGTAGGATAAGTGTGAGATTGCCGTTGCTGGCTGCCCCTATCTTATTGCCTCAACATGTTGGTGC ACTTATTTCGGTTCGGAATCCGGGGTGGCCCAGTTCCAGGATGGTCGCTACAATCCCTGCGCTTCCAGAC GTTCTCTGCCGCCAGGTCCTCAGATGACCGGCTCAGCTCACACCTCCTGCGGACTGTAGCCCAGCTCCGT GTACAGCTCCGGGCTCACCTCCCTCGAGCCCCACCAGCTTCCCACTGGAGCCCCTCTGCCTGGTGTTGGG TTGGGGGGACCTTGGTGGTCCCTGCAGTCCTGTGGCAGCATCCACGCCTCTGCCTTATAGCACTATGTGA GGCCAAAGAGTCCCCTCCTGCCCAGCCCACACGTGCCCCAGAGCTGCGCTTTAATTGGAAGCTCTTCTGG CATTTCCTTCACCCCCACCTGCTGGCCTTGGGGGCAGCCATTGTGCTGGCCTTAGGTGCGGCACTAGTGA ATGTGCAGATCCCCTTGCTCCTGGGCCAGCTGGTGGAGATTGTCGCCAAGTACACGAGGGACCACATGGG GAGTTTCGTGTCTGAGTCCCGTAAGCTCAGCGTCCAGCTGCTCCTACTGTACGGTGTTCAGGGACTGCTG ACCTTTGGATACCTAGTGCTGCTGTCCCACATTGGTGAGCGCATGGCCATGGACATGCGGAAAGCCCTTT TCAGCTCCCTGCTCCGGCAAGACATTGCTTTCTTTGATGCCAAAAAGACAGGGCAGCTAGTGAGTCGCTT GACTACTGATGTGCAAGAATTCAAGTCATCCTTCAAGCTTGTCATCTCCCAGGGACTGCGCAGCTGCACC CAGGTGATTGGTAGCCTGGTGTCCCTGTCTATGCTGTCCCCTCGCCTTACCCTGATGCTGGCTGTCGTCA CACCCGCCCTCATGGGAGTGGGCACCCTGATGGGCTCAGGCCTCCGAAAGCTGTCTCGCCAGTGTCAGGA GCAGATTGCCAGGGCAACAGGTGTAGCAGATGAGGCCCTTGGCAATGTTCGGACTGTGCGGGCCTTCGCG ATGGAGAAGAGGGAGGAGGAACGCTATCAAGCAGAACTGGAGTCATGCTGCTGTAAAGCAGAAGAACTGG GCAGGGGCATCGCCTTGTTCCAAGGGCTCTCCAACATCGCTTTCAACTGTATGGTCTTGGGCACCCTGTT CATTGGGGGCTCCCTTGTGGCTGGACAGCAGCTGAAAGGGGGAGACCTCATGTCCTTCCTGGTGGCTTCC CAGACAGTACAGAGGTCTATGGCCAGCCTCTCTGTCCTGTTTGGTCAGGTGGTACGTGGGCTGAGTGCGG GAGCCCGAGTCTTCGAATACATGGCCCTGAGCCCTGTCATCCCATTGACCGGGGGCTACTGCATCCCCAA CAAGGACATTCGTGGTTCCATCACCTTCCAAAATGTCACCTTCAGCTACCCCTGCAGACCTGGCTTCAAT GTGCTCAAGGACTTCACCCTGAAGCTGCCCTCTGGCAAGATTGTGGCTCTTGTGGGCCAGTCTGGGGGAG GAAAGACCACAGTTGCCTCCTTGCTGGAACGCTTCTATGACCCTGAAGCTGGCTCGGTGACGTTGGATGG GCATGACCTGCGAACTCTCAACCCCTCCTGGCTCCGGGGCCAGGTCATAGGTTTCATCAGCCAGGAGCCA GTCCTGTTTGCAACAACCATCATGGAGAATATCCGATTTGGGAAGCTGGATGCTTCCGATGAAGAGGTGT ACACAGCTGCACGAGAAGCCAATGCCCACGAGTTCATCAGCAGCTTCCCCGATGGCTACAGCACTGTGGT TGGTGAGCGGGGCACAACCTTGTCTGGTGGCCAGAAGCAGCGCCTAGCCATCGCACGTGCCCTCATCAAG CAGCCCACAGTGCTGATCCTGGACGAGGCCACCAGTGCGCTAGATGCAGAATCCGAGAGGGTGGTACAGG AGGCCCTGGACCGGGCCAGTGCTGGCCGCACCGTGTTGGTCATTGCCCACCGGCTTAGTACTGTCCGTGC AGCCCACTCCATCATTGTCATGGCCAATGGCCAAGTCTGTGAGGCTGGGACCCACGAAGAACTCCTTAAA AAAGGCGGGCTCTATTCAGAGCTTATCCGGAGACAAACCCTGGACGCCTCACTGACCTCAACCCCTCCTG CAGAGAAGCCTGAAGACCCCAAAAGCTGCCAGTCCAAAGCCTGAGAGGCCCCCTGGGGTCAGGTCACTGC AAGACATCAGTCTGAGGCTGGAGCTCTGCTGGGGACAGAGCCCTTGGAAGGCCAGTGTGCTGGAGTGTGG CCTGCACTACTGCCCCCTTAAAGGAGCTGGGCTCAACAGCTGGGGTGGGAAATGGCTCTCCGCCCACCTG CTCTGACCATCCTGACTCAGCTCTGTGGTCACGGGATGTACACAGTGAGACAGAGTCCCTGGACACCTCT GCTTGACCCTCTCAGGCTGGCCTGGTTCTGCCCAACACTCAGTTGTTTGGGCCCTCACTCCAGGAGACTG GCCAGGCCTGGTAGAATGGGTTGTAATCCCAGCACTTGGGAGACAGAAGTGGGAGGGTCAAGAGTTCAAG GCCATCCTGGGCTAAATAGTGAGACCCTCTCTCAACAAAAAGAAAAACCTAAATAAAGAGGAAAAATTTT CAAAGAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4237894 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4237894 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19622 same embryo
	EMAGE:19620 same embryo
	EMAGE:19623 same embryo
	EMAGE:19619 same embryo
	EMAGE:19624 same embryo
	EurExpress:euxassay_005065 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source