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EMAGE:19625

Med11 mediator of RNA polymerase II transcription, subunit 11 homolog (S. cerevisiae) ( MGI:1913422)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19625 EMAGE:19625 EMAGE:19625 EMAGE:19625 EMAGE:19625
"Pseudo-wholemount" of euxassay_006262. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006262_01 euxassay_006262_02 euxassay_006262_03 euxassay_006262_04
EMAGE:19625 EMAGE:19625 EMAGE:19625 EMAGE:19625 EMAGE:19625
euxassay_006262_05 euxassay_006262_06 euxassay_006262_07 euxassay_006262_08 euxassay_006262_09
EMAGE:19625 EMAGE:19625 EMAGE:19625 EMAGE:19625 EMAGE:19625
euxassay_006262_10 euxassay_006262_11 euxassay_006262_12 euxassay_006262_13 euxassay_006262_14
EMAGE:19625 EMAGE:19625 EMAGE:19625 EMAGE:19625 EMAGE:19625
euxassay_006262_15 euxassay_006262_16 euxassay_006262_17 euxassay_006262_18 euxassay_006262_19
EMAGE:19625 EMAGE:19625 EMAGE:19625 EMAGE:19625 EMAGE:19625
euxassay_006262_20 euxassay_006262_21 euxassay_006262_22 euxassay_006262_23 euxassay_006262_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19625Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19625_wholemount_strong.wlz
19625_wholemount_moderate.wlz
19625_wholemount_weak.wlz
19625_wholemount_possible.wlz
19625_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19625_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7505
Entity Detected:Med11, mediator of RNA polymerase II transcription, subunit 11 homolog (S. cerevisiae) ( MGI:1913422)
Sequence:sense strand is shown

>T7505
GGCGCCACACCTCGCAAAAGACGGCCTGTGGGGCTTTCCACTGGAACGATGGCTACCTACAGTCTGGCGA
ACGAGCGGCTGCGCGCCCTGGAGGATATCGAGCGCGAAATCGGCGCCATCCTCCAGAACGCAGGAACGGC
GATCCTGGAACTGTCCAAGGAAAAGACTAACGAGCGGCTCCTCGACCGGCAGGCGGCAGCCTTCACCACT
TCTGTGCAGCACGTGGAGGCAGAGCTATCCGCTCAAATCCGCTACCTCACTCAGGTGGCCACAGGGCAGC
CGCATGAGGGCTCCAGCTACTCTTCCAGGAAGGACTGTCAAATGGCTCTAAAGCGAGTAGACTACGCCCG
CCTCAAGATTAGTGATGTGGCGAGAACCTGTGAGCAGATGCTGGAGAACTGAGTTCGGATGATCCAGAGT
GTGCCCTGCACCAGAGACCGTAGGATGGGGAGCAGTGCATCATACACGTGCCCTACTGGTCAGGGCAGAC
GAGGACCACAAGGCAGAAAGGAGAACTGAAGAATCCAAAGCCCCAGTGTACACACATCTCTTCTGACTCT
TCCAGCAAGACGGCCCTGTTTTCCACTATGCAAGATCAGACAGAGGTCAGAGGTCAACTCCCAACCCACT
TCCTCATGTGCACCCTGCCCTGGAGTTTCAGAAGACTTGGCCCAATAAAAATAAATGTGTTCTTATAAGC
ACACACAGTGGCTCACACCTGTAGGCCCGGTACTTGGGAAGTAGAGGTGGGAAGATTGCTGTAGTTTGAG
ACCAACCTGGGCTAAGTACATAGAGGGACCCTCCTTCAAAAGGGGAAGTTATGTTCCTCATTGGAAGAAA
CAAAACAGGAACTAAGTGTTTTAATAATAAAAGCTGTATTTTTTTATAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4238297 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4238297 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19626 same embryo
 EurExpress:euxassay_006262 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS