EMAGE mouse gene expression spatial database

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EMAGE:19626

Chaf1b chromatin assembly factor 1, subunit B (p60) ( MGI:1314881)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005069. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005069_01	euxassay_005069_02	euxassay_005069_03	euxassay_005069_04

euxassay_005069_05	euxassay_005069_06	euxassay_005069_07	euxassay_005069_08	euxassay_005069_09

euxassay_005069_10	euxassay_005069_11	euxassay_005069_12	euxassay_005069_13	euxassay_005069_14

euxassay_005069_15	euxassay_005069_16	euxassay_005069_17	euxassay_005069_18	euxassay_005069_19

euxassay_005069_20	euxassay_005069_21	euxassay_005069_22	euxassay_005069_23	euxassay_005069_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19626_wholemount_strong.wlz

19626_wholemount_moderate.wlz

19626_wholemount_weak.wlz

19626_wholemount_possible.wlz

19626_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19626_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 10 11 12 13 14

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 09 15 16 17 18

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 11 12 13 14

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 12 13

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 10 11 15 16 17

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 moderate expression: see section 22 23

rest of cerebellum marginal layer

strong

regional

strong expression: see section 16 17 18 19 20 21 moderate expression: see section 04 05 06 07 08 09

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 19 20 21 moderate expression: see section 04 05

midbrain ventricular layer

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 moderate expression: see section 08 17

lower jaw incisor

moderate

regional

moderate expression: see section 10 11 14 15

lower jaw molar

moderate

regional

moderate expression: see section 06 07 18

upper jaw incisor

moderate

regional

moderate expression: see section 10 11 14 15

upper jaw molar

moderate

regional

moderate expression: see section 06 07 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7507
Entity Detected:	Chaf1b, chromatin assembly factor 1, subunit B (p60) ( MGI:1314881)
Sequence:	sense strand is shown >T7507 CGGAAACTCTGGAGGATGAAAGTGATCACGTGTGAAATCGCCTGGCACAACAAGGAGCCAGTGTACAGCC TGGACTTCCAGCATGGGGCAACCTGGAAGATCCACAGGCTGGCATCCGCGGGGGTGGACACCGCCGTCAG GATCTGGAAGTTGGAGAGGGGGCCAGATGGGAAAGCCATTGTGGAATTTCTCTCCAATCTTGCTCGACAC ACCAAAGCTGTCAATGTTGTACGCTTTTCTCCAACTGGGGAGATTTTAGCATCTGGAGGAGATGATGCCG TCATTCTGTTGTGGAAGATGAATGACAGCAAGGAGCCGGAGCAGATCGCCTTTCAGGATGAGGAGGAGGC TCAGCTGAATAAGGAGAACTGGACTGTGGTAAAGACCCTGAGGGGCCACTTAGAAGATGTGTATGATATT TGCTGGGCAACCGATGGGAATTTGATGACCTCTGCCTCTGTGGATAACACAGTTATCATATGGGATGTCA GTAAAGGGCAGAAAATTTCCATTTTCAACGAGCATAAAAGTTATGTGCAAGGAGTCACCTGGGATCCTTT GGGTCAATATATTGCGACCCTGAGCTGTGACAGGGTGCTGCGGATCTATAATACCCAGAAAAAGCGTGTG GCTTTCAACATTTCAAAGATGCTCTCTGGACAGGGGCCTGAAGGAGAGGCAAGAAGTTTCCGGATGTTTC ACGACGACAGCATGAAGTCGTTCTTCCGAAGGCTCAGCTTTACTCCAGATGGATCTTTGCTCCTCACTCC AGCCGGATGTATGGAATCTGGTGAGAATGTGACAAACACCACTTATGTGTTCTCCAGAAAACACCTGAAA AGGCCAATCGCCCATCTTCCGTGCCCTGGGAAAGCGACTCTTGCTGTCCGCTGTTGTCCTGTCTACTTTG AATTGAGGCCAGTGGCAGAAACAGAGAAAGCATCAGAGGAGCCCAGCCCCGAGCTGGTGAACCTGCCCTA CCGTATGGTGTTCGCGGTGGCTTCCGAGGACTCTGTGTTGCTCTATGACACTCAACAGTCTTTCCCATTT GGCTATGTGTCTAACATCCATTACCATACCCTGAGTGACATTTCCTGGTCCAGCGATGGGGCCTTCTTGG CCATTTCCTCCACGGATGGCTATTGCACATTTGTGACCTTCGAGAAAGGTGAACTTGGCATACCTTTGAA AGAGAAGCCAGTGCTGAGCATAAGGACTCCCGACACAGCCAAGAAAGCCAAGAACCAGACACACCAGGGA TCTTCGCCGGGATCCAGATCAGTCGAAGGGACCCCCAGCAACAGAACCCAAGACCCCAGCAGTCCCTGTA CCACTCCTTCACCAACCACACAGTCTCCAGCTCCGTCGGCCATCAAGGACAGTCCCTCAGCCATCCCGGC TGGCAAAAGCCCCTTGCCACAACCTTCTGAAGAGAAGACCCTGCAACCTGCTGGGCAAAACATGAAAGCC CCCCAACCCCGCAGGGTCACCCTGAACACGCTGCAGACTTGGGGCAAGACAGCCCCCCGGAGAATAAATT TAACACCATTGAAAACAGACACTGTGCCAAATCCACAGCCCAATAGCGGGACCGCCCCCTCCACAGAAGA GGTCCAGCCAGAGGCTCCTGGGGAGCCACCGGAGGAGCCTCCTGAACTGAAGAGACCCAGGCTTGAGGAA CGTGAAGGAGATGCCCAAAACCTGGCCCCTGATGACTCTTCCAAGACTGTCTGAGCCTGCTATGCACCTG CGTGCAGAGGAGGCAAGGCCAAAGCTCGAAGGCTGGTGATGGTGGCCAAATAAACACTCATGAATTGGTT CACATAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:4238801 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4238801 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19625 same embryo
	EurExpress:euxassay_005069 same experiment
	MGI:4823833 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source