EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19632

Xylt2 xylosyltransferase II ( MGI:2444797)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005092. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005092_01	euxassay_005092_02	euxassay_005092_03	euxassay_005092_04

euxassay_005092_05	euxassay_005092_06	euxassay_005092_07	euxassay_005092_08	euxassay_005092_09

euxassay_005092_10	euxassay_005092_11	euxassay_005092_12	euxassay_005092_13	euxassay_005092_14

euxassay_005092_15	euxassay_005092_16	euxassay_005092_17	euxassay_005092_18	euxassay_005092_19

euxassay_005092_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19632_wholemount_strong.wlz

19632_wholemount_moderate.wlz

19632_wholemount_weak.wlz

19632_wholemount_possible.wlz

19632_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19632_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon ventricular layer

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10 16 17 18 19 20

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 15 16 17 18 19

dorsal root ganglion

weak

regional

weak expression: see section 05 06 07 08 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7526
Entity Detected:	Xylt2, xylosyltransferase II ( MGI:2444797)
Sequence:	sense strand is shown >T7526 GATAGGGGCCCCAGGCTTCCCACAGCATGGAGACACAGGGAGTGTGGAGGGTGCCCCCCAGCCCACGGAC AACACCTTCACTCCGAAGTGTGAGATTGTGGGCAAGGACGCACTGTCAGCACTGGCCCGGGCCAGCACCA AGCAGTGTCAACAGGAGATCGCTAATGTAGTGTGCCTGCACCAAGCTGGGAACCTAATGCCCAAGTCTGT GCCCCGGCACTGCCAGCTGGCTGGCAAGATGAGCCCTGGCGTCCAATGGGAAGAGATCCGGGCCCAGCAG CCTGTTGGTGGCCCTCCAGTACGCATCGCCTACATGCTGGTGGTTCACGGCCGTGCTATCCGCCAGCTGA AGCGTCTTCTGAAGGCCGTCTACCATGAGCAACACTTCTTTTATATACATGTGGACAAGCGTTCCAACTA CCTGTACCGGGAGGTAGTGGAACTGGCCCAGCACTACGAGAATGTGCGGGTGACACCTTGGCGCATGGTC ACCATCTGGGGCGGGGCCAGCCTTCTGAGGATGTATCTGAGGAGCATGAAGGACCTTCTGGAAATTCCTG GCTGGACCTGGGACTTCTTCATCAACCTGAGTGCCACTGACTATCCAACGAGGACGAATGAGGAGCTGGT AGCATTCTTATCCAAGAACCGGGACAAGAATTTCCTCAAGTCACATGGGCGAGACAATTCCAGGTTCATT AAGAAGCAAGGCCTGGACCGGCTTTTCCATGAGTGTGATTCCCACATGTGGCGCCTGGGTGAACGGCAGA TCCCGGCAGGCATTGTGGTGGATGGTGGCTCTGACTGGTTTGTGCTGACACGCAGCTTTGTGGAATATGT GGTGTATACAGATGACCCCCTCGTGGCCCAGCTTCGCCAGTTCTATACATACACATTGCTTCCGGCTGAG TCATTCTTCCACACAGTGCTGGAGAACAGCCCGGCCTGTGCGAGCCTGGTGGACAACAACCTGCGGGTCA CCAACTGGAACCGCAAGCTGGGCTGCAAATGCCAGTACAAGCACATCGTGGACTGGTGCGGCTGCTCCCC CAACGACTTCAAGCCACAGGACTTCCTGAGGCTTCAGCAAGTCTCCAGACCCACCTTCTTTGCCCGGAAG TTCGAGTCGACTGTGAACCAGGAAGTCCTGGAAATTTTGGACTTCCATCTATATGGCAGCTACCCACCCA GCACACCAGCCCTCAAAGCCTACTGGGAGAACATCTACGACGTGGCCGATGGCCCTGGCGGACTCAGCGA CGTCCTACTCACAGCCTACACAGCCTTTGCCCGTCTCAGCCTGCGTCATGCTGCCACTGCTGTATCCCCA CTGGCCACTGCAGTCTGCAGGTTTGAGCCCAGGGGGTTGCCGTCCAGCGTGCACCTGTATTTCTATGACG ACCATTTCCAGGGCTACCTGGTGACGCAGGCGGTGCAGCCCTCAGCCCAGGGGCCAGCAGAGACACTTGA GATGTGGCTGATGCCCCAGAGGTCGCTGAAGCTGTTGGGGCACAGTGACCAGGCCAGCCGGCTCCAGAGT CTGGAGGTTGGTACTGAGTGGGACCCCAAAGAACGTCTCTTCCGGAACTTTGGGGGCCTGCTGGGACCAC TGGATGAGCCTGTAGCCATGCAGCGCTGGGCCCGGGGCCCCAACCTCACAGCCACTGTGGTCTGGATTGA CCCCACCTATGTTGTGGCCACATCGTATGACATCACGGTAGATGCGGATACCGAAGTCACGCAGTACAAG CCTCCACTGAGCCTGCCACTGCGGCCGGGAGCCTGGACTGTCCGCTTGCTTCAGTTCTGGGAACCCCTGG GTGAGACCCGCTTCCTCGTGCTGCCGTTGACGTTCAACCGCAAACTACCTCTCAGGAAAGATGATGCCAG CTGGCTGCATGCGGGACCACCCCACAACGAATACATGGAGCAGAGTTTCCAGGGCCTAAGTGGCATCCTG AATCTGCCTCAGGCAGAGGCCCTGGAGGAGGCTGCCCGGAGGCACACAGAGCTCACGGGTTCTGCACTTG AGGCCTGGACAGATGGGGAACTGAGCAATTTCTGGTCTGTGGCAGGATTGTGTGCCATAGGACCTTCTGC TTGTCCCTCCCTGGAGCTCTGCAGACTGACCAGCTGGAGCTCTCTGTCTCCTGACCCCAAGTCAGAGCTG GGGCCTGTCAAAGCTGACGGGCGACTCAGGTAGCAGGACCCCAGCCAGCACAACCCGGAGGAGCCGGGGA ATTGCACCTTACAGACAACGGAGGGACATCTCTCCCACAGACAAGAACCAGAGGCCCAGGCAGTTTGCCC ATTTGAGCCAACAGGAACCTACACTGAGGGCAGGGAAGGTGGGTGCAGTACTCCCTACTGCTAACGGCCC TGCAAGAGACCAGCGGTCCACTGGAGCCATGAGGAGGATCTTCCTCTGCTCCTCGTTAGTTTATACTTTT TTAATAATTAAAATGAGTGGCAGAGGGAGGACGATGTGCAATAGGGCTCAGAGCAGCCCCAGGATATGGG CCATGGCCATGGGGAGCACCACAGAGCATATCGGTTTCTGCTCTGGGGAGGCATGGGCTGCCTCCCTCTC CAGAGCCTCCATCCCCAGCCTGCATCTGAACTGCGCACTGCTGAGGCTCTGAGGCCCGGAGGACAGGTGA TCTGTGGGGGCTACTGTGGAGTTGGACTTTAGTGAGCAGGCCTCTCCTCTGGGAGCCAGGGAGCCCTAGA GCAGAGCTGGGGCCGGCTCTCGGGCTTCCCTAGGAAATGCGCGCCATCAGGGCCTGCTGAGATCCATGAA GTTTCCCTACTGGAGGAGGAAGCCCACTGGCGGCACAGGAAGGCTGTCTGTCAGAGCTCTCACAAGAGTC GAGTTGTGCCTGAAGCTCAGTGCGAAGTCTGAAATATGCAACAGAGGAAATATATCTCTATCTCTCCAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4239646 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4239646 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19633 same embryo
	EurExpress:euxassay_005092 same experiment
	MGI:4829248 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source