EMAGE mouse gene expression spatial database

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EMAGE:19633

Mesdc2 mesoderm development candidate 2 ( MGI:1891421)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005079. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005079_01	euxassay_005079_02	euxassay_005079_03	euxassay_005079_04

euxassay_005079_05	euxassay_005079_06	euxassay_005079_07	euxassay_005079_08	euxassay_005079_09

euxassay_005079_10	euxassay_005079_11	euxassay_005079_12	euxassay_005079_13	euxassay_005079_14

euxassay_005079_15	euxassay_005079_16	euxassay_005079_17	euxassay_005079_18	euxassay_005079_19

euxassay_005079_20	euxassay_005079_21	euxassay_005079_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19633_wholemount_strong.wlz

19633_wholemount_moderate.wlz

19633_wholemount_weak.wlz

19633_wholemount_possible.wlz

19633_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19633_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 08 09 11 12

hypothalamus ventricular layer

moderate

homogeneous

moderate expression: see section 11 12 13

diencephalon lateral wall ventricular layer

moderate

homogeneous

moderate expression: see section 11 12 13

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 11 15 16

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 13 14 15 16 17 18

pons ventricular layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14

midbrain ventricular layer

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 12 13 14

spinal cord ventricular layer

moderate

regional

moderate expression: see section 09 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7524
Entity Detected:	Mesdc2, mesoderm development candidate 2 ( MGI:1891421)
Sequence:	sense strand is shown >T7524 CGGACGCGTGGGTGGCTGCCTCCAGGTGGCTGCGCGCGGTTCTACTGTTTCTGTGTGCCTCGGACCTTCT GTTGCTCCCTCCTCCTAATGCCTACGCGGCGGACACTCCGGGCGAGGCCACCCCACCTCCACGGAAAAAG AAGGACATCCGCGATTACAACGATGCGGACATGGCGCGACTTCTGGAGCAGTGGGAGAAAGATGATGACA TAGAAGAAGGAGACCTTCCAGAACACAAGAGACCCTCAGCACCTATCGACTTCTCAAAGCTAGACCCAGG CAAACCTGAGAGCATCTTGAAAATGACAAAGAAAGGGAAGACTCTGATGATGTTTGTCACCGTGTCTGGG AACCCCACCGAGAAGGAGACAGAGGAGATCACCAGCCTGTGGCAGGGTAGCCTGTTCAACGCCAACTATG ACGTTCAGAGGTTCATCGTGGGATCCGACCGCGCCATCTTCATGCTCCGGGATGGGAGCTATGCCTGGGA GATCAAGGACTTTTTGGTCAGTCAAGACCGGTGTGCTGAAGTCACTCTAGAGGGACAGATGTATCCTGGC AAAGGAGGAGGAAGCAAGGAGAAAAATAAAACAAAGCCAGAGAAGGCTAAAAAGAAGGAGGGAGATCCCA AACCACGTGCTTCCAAGGAAGACAATCGAGCTGGGAGCAGAAGAGAAGACCTTTAGCAGGTGGCAAGCTT CCCGCCGGGTAGGCAGGACCAGATAGCCCCTCGCAGACTGGTGGGGCAGGTGGTGCAGGAAACTGCATAG CAGATGACATTCTCATGTCCTCTAGAAATGGTCTGTGTATGACCATCTGGTCAATATCACAGTTACCGTA TAAAAGGCTTCCCATGTGAAGGCAGGATTTGTGATTGGTAGTGGACTGGCAAGTCATGTCTACTGTGGCT TCACCTGACTTTGGAAACTTGATGTTTTGTACCTTTTACTTCTCTTGAGTCCATGACACCCCCAGAGTAC CACTCCCTCCTCAGTGCACCTGCTGTGGTCCCATGGCCTGTTGTATCTGTGTATTACAAGCTCAAGTCAG ACAAGCCTTGAGAAGCTTCGACTTGACTGAAGCGCGGGCATTGATTGTTCAGTGAGTGAAGATAACTGTG CCGTATGAGCAGTGCTGTGTTCAGTGAGTGAAGATAACTGTGCCGTATGAGCAGTGCTGTGTTCAGTGAG TGAAGATAACTGTGTCGTATGAGCAGTGCTGTGTTCAGTGAGTGAAGATAACTGTGCCGTATGGGCAGTG CTGTGCGCCCAGGATCTGGACAGCAAACTCGTTATGCAGAGTGGGCAGATTCACGACAGTGCCATCCAGA ACTTCTCATCGGTTCTGGGGAGTCTTTGTCTCCCCGTCTCCTGGAGTTGAAGAGGGAATGCGCTTGTTCA TCGTGTGAGGAAAGAAGGATGCTTCCACTGAGGGCCCACGTTCTGCATAGCCCAACAACTATAAAGTCGG CGAGGAGGGACCAGCAGAGTCTTGCATAGGAGTGGACCTCATCTACAGTATTTCTAAGCGGTTTCCTTTG ATGAAGTGCCTAAACTTGTGCTTTTGTATGGTACTCTCATGATGTGCTGCTGTTCACATCTGTTTCTGCT GTTCATAGGTTGAAGCTTCGCTAAAAGAACATTTCTATGAGGATGCCATTTTTAAGAACTCACATTCTTT TAAAACCTCAGTTGTGGTTTGAAATTCGAAATAGTACATGTACACGTACCCGGCTAGAAATTGTCACTGT TAAGAGTGTCACAGTGTGAAGAATTGCCCTACCCTGCTGCCAGTACTTTGTCCCAGTGAAGCTACTGTTG GTCATTGGTTTTTCAAAAATAAAGTACAATTACCAGCTTCCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4239248 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4239248 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19632 same embryo
	EurExpress:euxassay_005079 same experiment
	MGI:4826163 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source