Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:1964

Tbx2 T-box 2 ( MGI:98494)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:1964

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
This is a close-up view of the heart. For left side view, see EMAGE:1963 Image 1 shows the right hand side of the heart region of a TS15 embryo (this view is mapped). Image 2 shows the left hand side of the heart region of a TS15 embryo.
Expression Pattern Description
Spatial Annotation:
EMAGE:1964Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
1964_wholemount_strong_3D_1.wlz
1964_wholemount_moderate_3D_1.wlz
1964_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1964_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
outflow tract
detected detected
branchial arch
detected detected
expression is detected in the splanchnic/pharyngeal mesoderm.
Annotation Validation: submitter
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3531302
Entity Detected:Tbx2, T-box 2 ( MGI:98494)
Notes:The Tbx2 probe used in this study by Harrelson is described as follows, "The probe contains ~2kb of Tbx2 cDNA (full-length cDNA is ~2.3kb). An alkaline phosphatase-conjugated anti-digoxigenin antibody was used to develop the color from BM purple AP substrate (both from Roche)."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:ICR
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Zach Harrelson, Virginia Papaioannou.
Principal investigator:Virginia Papaioannou, Columbia University, 701 W. 168th St. Dept of Genetics & Development HHSC 1402, New York, USA NY 10032
Submitted by:Zach Harrelson, Columbia University, 701 W. 168th St. Dept of Genetics & Development HHSC 1402, New York, USA NY 10032
Experiment type:non-screen
References:[ doi:10.1242/dev.01378] [ PMID:15459098] Harrelson Z, Kelly RG, Goldin SN, Gibson-Brown JJ, Bollag RJ, Silver L 2004 Tbx2 is essential for patterning the atrioventricular canal and for morphogenesis of the outflow tract during heart development. Development (131):5041-52
Links:EMAGE:1963 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE