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EMAGE:19641

Olfr592 olfactory receptor 592 ( MGI:3030426)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19641 EMAGE:19641 EMAGE:19641 EMAGE:19641 EMAGE:19641
"Pseudo-wholemount" of euxassay_005101. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005101_01 euxassay_005101_02 euxassay_005101_03 euxassay_005101_04
EMAGE:19641 EMAGE:19641 EMAGE:19641 EMAGE:19641 EMAGE:19641
euxassay_005101_05 euxassay_005101_06 euxassay_005101_07 euxassay_005101_08 euxassay_005101_09
EMAGE:19641 EMAGE:19641 EMAGE:19641 EMAGE:19641 EMAGE:19641
euxassay_005101_10 euxassay_005101_11 euxassay_005101_12 euxassay_005101_13 euxassay_005101_14
EMAGE:19641 EMAGE:19641 EMAGE:19641 EMAGE:19641 EMAGE:19641
euxassay_005101_15 euxassay_005101_16 euxassay_005101_17 euxassay_005101_18 euxassay_005101_19
EMAGE:19641
euxassay_005101_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19641Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19641_wholemount_strong.wlz
19641_wholemount_moderate.wlz
19641_wholemount_weak.wlz
19641_wholemount_possible.wlz
19641_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19641_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6279
Entity Detected:Olfr592, olfactory receptor 592 ( MGI:3030426)
Sequence:sense strand is shown

>T6279
AGGTCTGGAGGAATCCATGCCTGGATTTCTCTGCCTTTCTGCTGTATTTATCTTGTGGCCCTAATGGGAA
ATACCATGATCCTGGTAGTTATCAAGACAGAGCGTTCTCTCCGAGAGCCCATGTTCTACTTTCTGGCCAT
CCTTTCTTCAGTCGACTTGGCTCTTTCTACAACCTCTGTGCCCCGTATGCTTGGCATATTCTGGTTTGAT
GCCCATGAAATAAACTTCGGTGGCTGTGTGGCTCANATGTTTTTGATCCATGCCTTTACTGGCATGGAGG
CTGAGGTTCTCATGGCCATGGCTTTTGACCGTTATGTAGCCATCTGTGCTCCACTCCACTACACAACCAT
CTTGACATCTCGTGTCCTGATGGGCATCAGCATATGTGTGGTAATTCGTCCAGCCTTGTTTATATGCCCA
ATGGTCTATCTCATTTACCGCTTACCCTTTTGTCAAGCTCATGTAATAGCCCATTCCTATTGTGAACACA
TGGGCATTGCAAAATTGTCCTGTGGAGACATCCATATCAACGCTGTGTATGGCCTTTTTGTGGTCTCTCT
CTTTCTTTTGAACTTGGTACTTATTGG
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:ATAATAGGAGCGTTTCTCACC; Reverse Primer - name:unspecified, sequence:ACATGTTCTCTTATCTGCTTTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19640 same embryo
 EurExpress:euxassay_005101 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS