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EMAGE:19660

Limd2 LIM domain containing 2 ( MGI:1915053)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19660 EMAGE:19660 EMAGE:19660 EMAGE:19660 EMAGE:19660
"Pseudo-wholemount" of euxassay_005173. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005173_01 euxassay_005173_02 euxassay_005173_03 euxassay_005173_04
EMAGE:19660 EMAGE:19660 EMAGE:19660 EMAGE:19660 EMAGE:19660
euxassay_005173_05 euxassay_005173_06 euxassay_005173_07 euxassay_005173_08 euxassay_005173_09
EMAGE:19660 EMAGE:19660 EMAGE:19660 EMAGE:19660 EMAGE:19660
euxassay_005173_10 euxassay_005173_11 euxassay_005173_12 euxassay_005173_13 euxassay_005173_14
EMAGE:19660 EMAGE:19660 EMAGE:19660 EMAGE:19660
euxassay_005173_15 euxassay_005173_16 euxassay_005173_17 euxassay_005173_18

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19660Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19660_wholemount_strong.wlz
19660_wholemount_moderate.wlz
19660_wholemount_weak.wlz
19660_wholemount_possible.wlz
19660_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19660_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
moderate moderate
regionalmoderate expression: see section 07 08 11 weak expression: see section 09
thyroid gland
moderate moderate
regionalmoderate expression: see section 07 08 11
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 09 13 weak expression: see section 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8853
Entity Detected:Limd2, LIM domain containing 2 ( MGI:1915053)
Sequence:sense strand is shown

>T8853
CGACCGACCCCCGCCCCGCGGCGCAGGCCGGGCCCGAACCCTCTTGGCTCGGCATCTCCTCCAGGGCCAC
CAAGCACCTCTGAAGAGCCATGTTCCAAGCTGCCGGAGCCGCCCAGGCCACCCCCTCTCATGAAGCCAAA
GGCAGCAGTGGCAGCAGCACGGTACAGCGGTCTAAGTCCTTTAGCTTGCGGGCTCAGGTGAAGGAGACCT
GTGCAGCCTGCCAGAAGACTGTGTACCCGATGGAGCGGCTGGTGGCAGACAAGCTCATTTTCCACAACTC
TTGTTTCTGTTGCAAACACTGCCACACCAAACTCAGCCTGGGCAGTTATGCTGCAATGCACGGTGAATTT
TACTGCAGACCTCACTTTCAGCAGCTGTTTAAGAGTAAAGGCAACTACGATGAAGGGTTTGGTCGTAAAC
AGCACAAGGAGCTCTGGGCCCACAAGGAGGTGGACTCAGGCACCAAGACGGCCTGAGACCCCTTTAACAC
CCATTCCCTCCCAGCACATGGCCTCCCGCTGGGCAGTGGAAAGGAGATTAACCCGGGGGCGCGGGGTGGG
AGAGGATGAGGCTCCCTCACACAGGTTTCAGGCATAAGGCTCTGCTCCAGGATTCCTTACTTTTCCCATG
GGAGGTTGGCGTTGGGAACCAGAATTGGAATTTTCACCATACTGTGTCCTTTAGTCCACCTCATCTCACC
CCACGGCTCCCTGGGAGGCCCACAAGCCCAGCTTCCCTACTTAGGTGCCTTTTCTCCAGCAAGGAGTCAG
CATGCCCTCCTCAGGGTCCCAAGCTCCCTCACTGCCACCTGGGCCTTGTGTACCCCCTTGTCTCCCCATC
TACCTCTGCCCCTTAGCCTGGTAATGAGCCACAGAGACTGGAAGAGGGAGAGTGCCATCTACTGGGCCTC
ATAGATGCCACCTCGCTGAGGGGGGAGGGCTGGGGAAGAGGCAAGACAGCCTGCAGCCTTCAGGGTCTGG
GGGTCCCTTGCACCACAAAGCTAAAGCTCTTGCTAGAGCCTCAGCTGACAGGGTCGGCAGTAGCTATGCT
CCTCCATCTGTTGTGCTGTTCTGTTGTGATCAACCCTCTTTTAAAAACATTTAAACAGCTCTTGACTCTC
TTGTGTGGCCTGATGGGGATGCTTTGTGGCAGGCATGCAGCAGACTCCTCATAGCGAGGGGAATGGAGGA
CAGCCCACAATACTCCTGGCCTTAAAGGGGCTGGTGGTCTGTGCCACCTTGGGGTGTTAAGCTGTCAAAC
CTGAAATCCCACTGTGTATCCTCGTCCCAGAGTAGCCAGGCACACTGGCTTGCAACCACACAAATGTTTT
GGGTTTTTGAGACAGGGTCTCACTGTAGCTCTGATTGGCCTAGAACACAGAGATCTGCCTGCCTCTGCTT
CCAAAGTGCTGGGATTAAAAAGGCCTGTGTCATCCATGCCTGGCTTCGGTTCTTCAGAGGTTAATTCACT
ACACTTGAGTACAGAAGAGAACCTTTCTGACTTGGCCAAGACCTTAGTGTCTGCCTTTCTGCTAGCTGAT
TCCAGCTGTCCAATTTCCTCCTAGTCCTTTACCCTAGAAATCTGCAAAGAATGGTAGGAATCCATAGCCT
ATCAAGTGCTGCCAGTACAACGTCTTCCACCTTCTCTCCTCATGACTGTCATCTTCCTGGAGCCTCTTGT
ATGAAGTGGGATGTCACAGCTACTGTGCCAGGACCTCCAGGGGCAAACTTTGCGCTCCTCAGAGTTCATG
CACACTGAGGGACGACATCACACAGCAGAAGCACAAGGCAAGTGTGAAGATGCTCCCTCAGTGGAAGTCT
GAGTTGTCTCGCCCCAAGCCCTATTTTCCTGGCTTCTGCAAACTGCCTATGGGACAGATGAATCGGTAAG
CAAGAGGTCAAAGGCTGCTAGGCAGCCCGGCCTCTGGTCTTGCTAATGGGTGAATCACTGTAGACTTCCT
TCAGGTGGGCATCTGGGAAGCAGGCACGACAAAGCCTTGGATTTATGGGACAAGGATGGTCTCTTGCTGC
AGTAAAGAAACAGTGGCTGGGCTGGACGTGGCAGCACATGCTTTTAATACCAGCACTCAGGAGGCAGAGG
CAGGTGGGTCTCTGTGCATTCAAGATCAGCCTGTTCTATGTAGTGAGTTCCAGGACAGCAGAGCTACATA
GTGAGACCTTGCCTCTGACTGATGTTCTGTAAGGGACCAAGGGAAACCCCTCGGCCAGGAAGACTACAAA
ATGCCATGAATGGTCCTAGGAACTCAGCTATTGATGGATTCGGCTCAGATGGACAACCAGTGCTGTCCCA
GTGACGCGCTGGGATAAAAGGGAAGGAGAGGGGCCACCTTGGCCAAGTTATCACAGGCTGCTTTTGGCAG
CCATTTCAAGTTCTATTTAAGAGGCCTCAACTTGGGTGTTAGGCATTGTACCCAACCCATCCCTGTAAAA
ATTCCCACCACCACCTTTAACCGAAAGCATGGTCACAGGACCCTTCTGATATAAATGATCCCTTTTATTG
TAAGTAATGCACTGGCCTGGCTTTGCACTGCAAGCCCTCAGTCAAGATATAGTCAATAACTATGGCTGCA
GGTTCTTCTGCACATTCCACAATTCAGACACAGAGCTGGGCAAGTGGGAGGGAGGGGAAGGAGGGTGGCA
GCGTGCACCTGACCCAGGCTTGGCCCTTCCATGTGGGGTTGGTGAAGGCAGATAAACCCCATGGAATGAA
GAACAAATGAAGGTGGTGTAGCATCTTTGCACAGCAGGCCAAGGGCTGCTAGGAAACCTTCCTTCATAAC
CTTTGGGCCCAGGCCACCCTGGAGGCCCACAACTTGTCATTTGGATTTGTTAATAAGAAAAAAATAAATT
AACAGCTTGGTCCTGAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6413206 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6413206 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19663 same embryo
 EMAGE:19662 same embryo
 EMAGE:19661 same embryo
 EMAGE:19659 same embryo
 EurExpress:euxassay_005173 same experiment
 MGI:4825926 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS