EMAGE mouse gene expression spatial database

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EMAGE:19664

Bex1 brain expressed gene 1 ( MGI:1328321)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006276. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006276_01	euxassay_006276_02	euxassay_006276_03	euxassay_006276_04

euxassay_006276_05	euxassay_006276_06	euxassay_006276_07	euxassay_006276_08	euxassay_006276_09

euxassay_006276_10	euxassay_006276_11	euxassay_006276_12	euxassay_006276_13	euxassay_006276_14

euxassay_006276_15	euxassay_006276_16	euxassay_006276_17	euxassay_006276_18	euxassay_006276_19

euxassay_006276_20	euxassay_006276_21	euxassay_006276_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19664_wholemount_strong.wlz

19664_wholemount_moderate.wlz

19664_wholemount_weak.wlz

19664_wholemount_possible.wlz

19664_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19664_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

head mesenchyme

weak

regional

weak expression: see section 01 02 03 04 05 06 07 12 13 14 19 20 21 22

vertebral axis musculature

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

facial vii ganglion

weak

regional

weak expression: see section 03 04 16 17 18

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 04 05 14

trigeminal v ganglion

weak

regional

weak expression: see section 02 03 04 05 06 07 15 16 17 18 19 20

cervico-thoracic ganglion

weak

regional

weak expression: see section 06 07 11

cervical ganglion

weak

regional

weak expression: see section 06 07 12 13

thoracic ganglion

weak

regional

weak expression: see section 09 10 11

dorsal root ganglion

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12 13

tongue muscle

weak

regional

weak expression: see section 09 10 11 12 13

bladder

weak

regional

weak expression: see section 10 11 12

tail mesenchyme

weak

regional

weak expression: see section 11 12 13 14 15

midgut loop lumen

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9664
Entity Detected:	Bex1, brain expressed gene 1 ( MGI:1328321)
Sequence:	sense strand is shown >T9664 AGCCCACGCAGGCTCACACCCCTCGCTGGGTCTCGGACCTGCCCTCGCTCACTCTCTCCAGCCCGCCATC CCTAACGGAGGCACCTGTTCCTGGTGGCGCAGCTCCTGGTGGCGCAGAGCACGCGGGCCCGGGCGGCGCG GAAAGCAGCAGGAGGAGGAAGAGCGGAGCAGGTCTGAGAAGCAGAAGCTTCGCGGCGCACCTGGTGGTGA GCATCTCTAGAAAGAGGAGAAGGCAAGGATAGGCCCAGGAGTAATGGAGTCCAAAGATCAAGGCGTGAAA AATCTCAACATGGAGAATGACCATCAGAAAAAGGAGGAGAAGGAAGAAAAGCCACAAGATACCATCAGAA GGGAGCCAGCTGTGGCCCTGACCTCCGAGGCTGGCAAAAACTGTGCACCTAGAGGAGGTCGCAGGCGGTT CCGGGTTCGGCAGCCCATCGCTCACTATAGATGGGACCTGATGCAGAGGGTTGGGGAGCCCCAGGGAAGG ATGAGAGAGGAGAACGTACAGAGGTTTGGGGGCGATGTGAGACAGCTCATGGAGAAGCTGAGGGAAAGGC AGCTGAGCCACAGCCTGCGGGCGGTTAGCACTGACCCGCCTCATCATGACCACCATGATGAGTTTTGCCT CATGCCCTGAGTCCTGAGCTTTTCTCTGAAGATGACATGGGGACCCCTGCCTTGTGATGTACAAATTTTG AGGTTACTCCTTTTTATGTAGATCTCTCCCTGTTACCAGCTTCTAATTGAAACTTGTGGTTTTTACTCGG CCTATAATTCTTTTGTTAGCAGAATTTATCAATTGCATGGGAAGATCCGTATTACCTATTGTGAACCTAA TAAAGCAGATTTAAAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6594252 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6594252 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19668 same embryo
	EMAGE:19667 same embryo
	EMAGE:19665 same embryo
	EMAGE:19666 same embryo
	EurExpress:euxassay_006276 same experiment
	MGI:4823459 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source