EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19665

Tra2b transformer 2 beta homolog (Drosophila) ( MGI:106016)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006134. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006134_01	euxassay_006134_02	euxassay_006134_03	euxassay_006134_04

euxassay_006134_05	euxassay_006134_06	euxassay_006134_07	euxassay_006134_08	euxassay_006134_09

euxassay_006134_10	euxassay_006134_11	euxassay_006134_12	euxassay_006134_13	euxassay_006134_14

euxassay_006134_15	euxassay_006134_16	euxassay_006134_17	euxassay_006134_18	euxassay_006134_19

euxassay_006134_20	euxassay_006134_21	euxassay_006134_22	euxassay_006134_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19665_wholemount_strong.wlz

19665_wholemount_moderate.wlz

19665_wholemount_weak.wlz

19665_wholemount_possible.wlz

19665_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19665_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9663
Entity Detected:	Tra2b, transformer 2 beta homolog (Drosophila) ( MGI:106016)
Sequence:	sense strand is shown >T9663 AGACCGGTGCGGAGGTGGTGCGGAGCGTGTCGGCTTCGAGCTCCTCGCAAAAGTGTGGCGTCGCGCTAGG CTGCGGCGGAGCCTCTGTAAGGAAGGCGGGAGCTTGACAGCTTCAGGAAAGGCCCGTAGCGGTGACAGCG ACCCGGAGTCATGAGCGACAGCGGCGAGCAGAACTACGGCGAGCGGGAATCCCGTTCTGCTTCCCGAAGT GGAAGTGCTCACGGATCGGGGAAATCTGCACGGCATACACCTGCAAGGTCTCGCTCCAAGGAAGACTCAA GGCGTTCTAGATCAAAGTCCAGGTCCAGGTCTGAATCTAGGTCTAGATCCAGAAGAAGTTCTAGAAGGCA TTATACAAGGTCACGATCACGATCTCGCTCGCATAGACGATCCCGGAGCAGGTCTTACAGCCGAGATTAT CGCAGGCGCCACAGCCACAGCCATTCTCCCATGTCTACTCGAAGGCGTCATGTTGGGAACCGGGCAAATC CTGACCCCAACTGTTGTCTTGGCGTGTTTGGGTTGAGCTTATACACCACAGAAAGAGACCTAAGAGAAGT GTTCTCTAAATATGGCCCCATTGCTGATGTGTCTATTGTATATGACCAGCAATCTAGACGTTCAAGAGGA TTTGCCTTTGTATATTTTGAAAATGTAGACGATGCCAAGGAAGCTAAAGAACGTGCCAATGGAATGGAGC TTGATGGGCGTCGAATTAGAGTCGATTTCTCTATAACAAAAAGGCCCCATACCCCAACACCAGGAATTTA TATGGGGAGACCCACTTATGGCAGTTCTCGCCGCCGAGACTATTATGACAGAGGGTACGATCGGGGTTAT GATGACCGGGACTATTACAGCAGATCATACAGAGGAGGAGGTGGTGGAGGAGGTGGATGGAGAGCAGCTC AAGACAGGGATCAGATTTACAGAAGACGGTCACCTTCTCCTTACTACAGTCGTGGAGGATACAGGTCACG TTCTCGATCACGATCCTACTCACCTCGTCGCTACTAAAGCATGAAGTTGAAGACTTTCTGAAACCTGCCA TAGAGCTGGGATATTGTTTGTGGACAATATTTTCTATTGTCTCCTGTTTAAAAAGTGAACAGTGCCTAGT GAAGTTAGGTGACTTTTACACCTTTTATGATGACTACTTTTGGTGGAGTTGAAATGCTGTTTTCATTCTG CATTTGTGTAGTTCGGTGCTTTGTTCAAAGTTAAGTGTTTTCAGAAAAGTATGTTTTGCATGTATTTTTT TACAGTCCAAATTTTGACTGCTGAGAAGTTTCTATTGTAAAAAACTTCATTTAAAAGGTTTTTCTACTGA ATCCAGGGTATTCTGAAGATCGAAGCCTGTGTGTAAAATGCTACCAGATGGCAAAAAGCAACAATAAACA AGTTTGATTTTTACTTTTCTTTCTAAATATCAATGCTTAACCAGAACCATTCTTAAGTTATCAGTAAAAA AGTAAAAAGTTAAATAAATGTCTTTCAGGAGTCCATGAAACTTGCCATATTCAATATACCTGCGATTAAG TGTTAAAAAATACGCTGTAACTCTGTACTGCTAGTATTAGAACAAAACTCTTCCCATACAGCAAATGCTT AATGCTTACATTAATGTGGATGTGTCGGCCTTTATGTAATCTGTATTATATATAGCAGGGAATAAGAGCT ACAGATACATGCCTTTAAAGGGCTGTTTCTTAAGGCTGTTACAAGGAGATAATGGTATTTCAACTAATTA TCAGCAAGTGACAATACATTCCACCACAGATGGACTCTTGTTCTTCTAGCGTTTAGACTATATGAAAATA CTGGGTGCTTCAAAGTATGGAAGGAGCGAAGGGATCATCAAACCTTGTCATGGATGAAGACTGCCTGTTC ATTTTTTAAATATGATTTTCAGGTCCTTTGCTTACCAAAGGAGGCCCAATTCACTCAAATGTTTTGAGAA CTGTTTAAATAAAGGCAAGAAAAATTGCTGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6594226 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6594226 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19668 same embryo
	EMAGE:19667 same embryo
	EMAGE:19664 same embryo
	EMAGE:19666 same embryo
	EurExpress:euxassay_006134 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source