EMAGE mouse gene expression spatial database

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EMAGE:19666

Atl1 atlastin GTPase 1 ( MGI:1921241)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006279. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006279_01	euxassay_006279_02	euxassay_006279_03	euxassay_006279_04

euxassay_006279_05	euxassay_006279_06	euxassay_006279_07	euxassay_006279_08	euxassay_006279_09

euxassay_006279_10	euxassay_006279_11	euxassay_006279_12	euxassay_006279_13	euxassay_006279_14

euxassay_006279_15	euxassay_006279_16	euxassay_006279_17	euxassay_006279_18	euxassay_006279_19

euxassay_006279_20	euxassay_006279_21	euxassay_006279_22	euxassay_006279_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19666_wholemount_strong.wlz

19666_wholemount_moderate.wlz

19666_wholemount_weak.wlz

19666_wholemount_possible.wlz

19666_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19666_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9667
Entity Detected:	Atl1, atlastin GTPase 1 ( MGI:1921241)
Sequence:	sense strand is shown >T9667 AGAATCTGAGCGAACTGCGCCCAGAACGGGCACAGAGCCTCTTAACTGCCAGAAACCTGCGGGGCCCTGG AGAAGACCGCAAGAGCAGTGACAGCGCCTCGCCACACCAGCCCACAGACCGCCATGGCTAAGAGCCGCAG GGACAGAAACAGTTGGGGTGGGTTTTCAGAGAAGTCAAGTGACTGGAGCTCAGAAGAGGAGGAGCCAGTG AGAAAGGCAGGGCCTGTCCAGGTCCTCATCGTCAAGGACGACCATTCCTTTGAGTTAGACGAGGCTGCGC TGAACCGCATCCTTCTGTCCCAGGCCGTCAGAGACAAGGAGGTGGTTGCTGTGTCTGTTGCTGGAGCCTT TAGAAAAGGGAAATCCTTCCTGATGGACTTCATGTTGCGGTACATGTACAACCAGGAATCTGTTGATTGG GTTGGAGATTACAATGAACCATTGACTGGATTTTCATGGAGGGGTGGATCGGAACGAGAAACCACAGGAA TCCAGATATGGAGTGAAGTTTTCCTCATCAATAAGCTTGATGGTAAAAAGGTTGCAGTGTTACTGATGGA TACTCAGGGAACCTTTGACAGTCAGTCAACTTTGAGAGATTCGGCCACAGTATTTGCGCTTAGCACAATG ATAAGCTCAATACAGGTCTACAACTTGTCCCAGAATGTCCAGGAGGATGATCTCCAGCACCTGCAGCTTT TCACTGAGTATGGCAGGTTGGCAATGGAAGAAACATTCCTGAAGCCTTTTCAGAGTCTGATATTTCTTGT TCGAGACTGGAGTTTCCCATACGAATTTTCATATGGAGCTGATGGTGGCGCCAAGTTCTTGGAAAAACGC CTCAAGGTCTCAGGGAACCAGCATGAAGAGCTGCAGAACGTCAGGAAGCACATCCACTCCTGCTTCACCA ACATTTCCTGCTTTCTCCTACCTCATCCTGGCTTAAAAGTCGCTACCAACCCAAACTTTGATGGGAAACT AAAAGAAATAGATGATGAATTCATCAAAAACTTGAAAATACTGATTCCATGGCTCCTGAGTCCTGAGCGT CTAGATATTAAAGAGATCAATGGGAATAAAATCACCTGCCGAGGCCTGCTGGAGTACTTCAAGGCATACA TAAAGATATACCAAGGTGAAGAATTGCCGCATCCCAAGTCCATGTTGCAGGCCACAGCAGAAGCTAACAA TTTAGCAGCTGTTGCAACTGCCAAGGACACATACAACAAGAAGATGGAAGAGGTTTGTGGTGGTGATAAA CCATTTTTGGCCCCTAACGACCTGCAAAGCAAGCACCTGCAGCTAAAAGAAGAATCTGTGAAGTTATTCC GAGGCGTGAAGAAGATGGGCGGGGAAGAGTTCAGCCGGCGTTACCTGCAGCAGCTGGAGAGTGAAATAGA TGAACTCTACATCCAGTATATTAAGCACAATGACAGCAAAAATATCTTCCATGCTGCTCGAACCCCAGCC ACACTGTTCGTTGTCATCTTTATCACATACGTGATTGCTGGTGTGACTGGATTCATTGGTTTGGACATCA TAGCTAGCCTGTGCAACATGATCATGGGACTGACGCTTATCACCCTCTGCACCTGGGCATATATCCGCTA CTCTGGCGAATATCGAGAGCTGGGTGCTGTAATCGACCAGGTAGCTGCAGCTTTGTGGGACCAGGGAAGC ACAAATGAGGCGTTATACAAGCTTTACAGTGCAGCAGCAACCCACAGACACCTGTGTCACCAAGCTTTCC CTGCACCCAAGTCAGAGCCTACTCAACAACCGGAAAAGAAGAAAATTTAATCTAAAAACATGCAGGTGCA TGACCGTTTGTCAGCTAACTATCAAAGCTTTTGTTTCCATGCAAACATGCAAAGTGCTTCCTTTAGAGTG AAACCCAAACACCCGACCGAGGACTCGGACAGTTTACTTCTTTACTCCAGTGTTTAATGAGCAGATGTAT GTATGCATGGTTATTTGTCAATGTGCAGTTTCCTCATTTCTGTCTTTAAATATGCTATTATCATTTCAGG TATTTGTAAATTTGAGGATAGATTTGTTCTGCTTGACTTTTCTAAGTTCTACTACTGGGTTATAATTAAA TATTGTGTAGTATTACTCTGGGTTTGGATATGCTCATTTGATTTCTATAGAAAATTTAAGTTATTTTACA TAGCTATTTGTTAAAGCACAGTATAACTTGGCAGGCTTGATTTTAATCTATATAATATTATACATACCAC AGGCTCTTACCTTGAAACATATTTGAGAGCTTAAAAGTTGCTTTACCAAAAACTATACATTGTTTCTTTT ATATCTTTATGAATATAGAATGTAAATCTCTTGATCAAAAATGCACAAAAATTGTTTTGTACATGTGTTT GAGTTTCACTGCACTGTATATTTTTTCATTTGGTACACCACAAATGTATTCTTCATAGGTTTATTCTTTT AATATGTGAACTATTAAACTTTACTGTTTCCTAACATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6595181 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6595181 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19668 same embryo
	EMAGE:19667 same embryo
	EMAGE:19665 same embryo
	EMAGE:19664 same embryo
	EurExpress:euxassay_006279 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source