EMAGE mouse gene expression spatial database

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EMAGE:19667

Ccdc43 coiled-coil domain containing 43 ( MGI:1289318)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006117. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006117_01	euxassay_006117_02	euxassay_006117_03	euxassay_006117_04

euxassay_006117_05	euxassay_006117_06	euxassay_006117_07	euxassay_006117_08	euxassay_006117_09

euxassay_006117_10	euxassay_006117_11	euxassay_006117_12	euxassay_006117_13	euxassay_006117_14

euxassay_006117_15	euxassay_006117_16	euxassay_006117_17	euxassay_006117_18	euxassay_006117_19

euxassay_006117_20	euxassay_006117_21	euxassay_006117_22	euxassay_006117_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19667_wholemount_strong.wlz

19667_wholemount_moderate.wlz

19667_wholemount_weak.wlz

19667_wholemount_possible.wlz

19667_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19667_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9662
Entity Detected:	Ccdc43, coiled-coil domain containing 43 ( MGI:1289318)
Sequence:	sense strand is shown >T9662 GGGTCCGGCAGGACCCTGTCCCCAGCATGGCGGCGCCCAGCGAAGTGGCCGCGGCAGTCCTCGGTGAAGG CGATGGCGGGGCTTTTGGATCCTGGCTTGACGGACGGTTGGAGGCGCTGGGAGTGGACCGAGCCGTTTAC GCCGCTTACATCCTTGGCGTCCTACAGGAGGAGGAGGAGGAAGAGAAGCTGGACGCCCTGCAGGGTATCC TCTCTGCTTTCCTGGAAGAAGAGTCTCTCCTTGATATCTGTAAGGAAATTGTGGAGCGATGGTCAGAAAC ACGGGATGTCACCACCAAAGTCAAAAAGGAAGATGAGGTGCAGGCCATCGCCACCTTGATTGAGAAGCAG GCACAGATTGTGGTGAAGCCCAGGGTGGTTTCAGAAGAAGAGAAGCAGAGGAAAGCAGCCCTGCTGGCCC AGTATGCCGACGTGACAGACGAAGAGGATGAAGCAGATAAGAAAGATGATGCGGGTGCCTCAACAGCAAA CGTCAGTTCTGACAGAACCTTGTTCCGAAACACCAATGTGGAAGATGTTCTCAATGCTCGGAAATTGGAG AGAGACTCACTTCGGGATGAGTCCCAAAGGAAGAAGGAACAGGACAAGCTGCAGAGGGAGAAGGACAAAC TAGCCAAGCAGGAACGCAAGGAGAAGGAAAAGAAAAGGACACAGAAAGGCGAACGGAAGCGATAACCTTG GCCCACTCTGCTCCTTCCACCCATGAATTGTGTGTCTGTGTATTTAAGAGAACTGAGACTAATGTATTTT CCAAAATTTCCTAAGGCTTTCCCCCGTTGTTTACATGTTGAAAAGGAAAATCACAATCACTTGTAAATGT GCCATGCTCTGGTGGTGTGGGCAGTCAATTATTGTTAGAATCTTTACCAAAGATCTGGAGTTAGGGTAGC CTTTCTATTTTAGTACTGAACTCCTGTGCCCTTCTTTTTTAAAAAGGTCTTCCCTCATTTTGTATATTTA GCCAGATACAGAAAGCTCACATCCTGAAGGAAACATGACCATTCTGAGTTTAAAAAAAAGTACAAATTGC TTCTGAGGCACTATTTGCCCTAAAATATAATGGGCTTTTGTTTTGAGACTGGGTTTCATTCTGTAGCCCA GGCTAGCCCTGAACTTGTTACAGTGTCCTTTTTTTCTCAGCTTCAGGATTATGGACATAGATCTCCACAC CTGGCTTGTAACTATTTTGAGGCTGAAAGAGCTAATGAAGGGCCCTGCCTAGATCCAGACTTTCTGACAT CAAATTAGGGAAGTGGAGGAAATTATTTGGAATGTGATCTAAGTTTTTGTTTTTTTTTAATGTGTCAACT TGGGCTCAGGCTGTAGTTGTGTTGCGTGCCTGCTTAGCATATACAAGGGCCTGGGACTGGGCCCCAGTAT GGAAGAAAGACTTAACCTGTGGGAAAAACAATTATCTTTTTAAATAGAGAGCTATGTATTTTTCATGTAA TCTGTGATTTCAAGAAGATGATTGGAGTATAAGGATTATCATTTTGTTAGGTAGAGTTTCTTTTTTTCTT TTTGTAATCATGAGTTCCCTTGACTCCCCTCTTGATTTTCTATCTTGATTTATCCTGCAGGCATGTCCTA CCTTTTGACATTGTGACTTGGCTTTGTTAAAAACTTGCAAAAAAAATCATGTTTTAAATGTTATGATTTT GTGTTAGGCTGAATTTATAGCTGTCCTTGACCTCGGGTTGGACACACCTGCAACATGAATAGTAAGACCT AATAATTCCTGGCTTAAAGAAACTGAAGGAATTACTCTTTTAGAGAAAAACACTGGAAATTTTGCTAACA CAATACTTAAAAGTATATAGTTGGTGGTTAAGCTTATGAGATGAGTGAATGGAAAACCTGGGAGAGGAAA GGGTGGACAGAATTCAAGTCTGCTTCTGTCCCAGCCACATCCACTTGTCAATAAAGGCCTTCTGTACCTG ACTTTGGGCAACAGGTATCAAAAGCCTCAAGAGAACAGTTTCTCTGATAAGATTATTTTATACCAGGGGT CAGAAGACTCCACTCACGGGCCAAGTTTGGCCCAGTCATTTTTGTATGGGGAAAAAACTAAAAAGGTTTT TTACATTTTTAAAAGGAAGACATTCGTGACTGAGACCATACGTGGCCTATAAACCTAGCATTTGGAGAAA GTTTGCTGACTTTTGTAACATTGGCTGCCTTTGTGCAGAAGAAAAAAACATGGTAGGGAAGGAGCTCTGC TGCCTCTTTGCCCTACAACCATGGCATTGCAAAAATAACCACCTTTTTCTTTTTTTAAATAAACTTTATT TAAGTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6594359 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6594359 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19668 same embryo
	EMAGE:19665 same embryo
	EMAGE:19664 same embryo
	EMAGE:19666 same embryo
	EurExpress:euxassay_006117 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source