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EMAGE:19689

2200002J24Rik RIKEN cDNA 2200002J24 gene ( MGI:1916397)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19689
euxassay_006418_01

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19689Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19689_wholemount_strong.wlz
19689_wholemount_moderate.wlz
19689_wholemount_weak.wlz
19689_wholemount_possible.wlz
19689_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19689_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T9700
Entity Detected:2200002J24Rik, RIKEN cDNA 2200002J24 gene ( MGI:1916397)
Sequence:sense strand is shown

>T9700
AGATTGCCAAGGCCATCTCAGGGACTGAGGAGTGAAGAGAAGACCTACAGACCTTGAGTTGGGTCTTCTT
ACAAAGTGGCTCAGGCTGCCCTTCAACACATTTTGTAGTCCAGGCCAAGATCCATGGGGGTTGGGACCAA
AATCAAGAACCTGGAAGCCCAGATCGCATCAGTGCAACCGGCCTATCAGAGATTGCCCAGGTCAGAGACA
GTCATGCTGATTGCCTTGCCTGCCCCAGCGAAGGATTACCTCTGTCTGTCCATCACAGCCTTCTGCTTCT
GCATCCTGCTGGCGATTCCAGCCCTGCTGTTCTCCCTTAAGACCCGGGAAGCCAACTCTGTTGGAGATTG
GAGGCGAGCCCAGAGGAACTCCACGCTGGCCTTGGGCTTCGGTGTCTCCAGCATCCTTGTGGGATGCATT
TTGATGGCCAGCTCTATTAGATTAATTAGTCTTTGAAACAGAGGGTATGACAACCCTCCTGAAGGCTCAC
CTCATTCAGGGAGTTCCAATCATTCTTGGAGCCTCTGATCTTGGACATCAGCAGCTGCCTGTGCTGAGGG
AACCCTCAGTTGGGCAACAATGTTAAGAACAATAAAGTTTTTGGGCTTCCAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6705641 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6705641 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19687 same embryo
 EMAGE:19690 same embryo
 EMAGE:19688 same embryo
 EMAGE:19691 same embryo
 EurExpress:euxassay_006418 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS