EMAGE mouse gene expression spatial database

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EMAGE:19690

BC061237 cDNA sequence BC061237 ( MGI:2682320)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006341. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006341_01	euxassay_006341_02	euxassay_006341_03	euxassay_006341_04

euxassay_006341_05	euxassay_006341_06	euxassay_006341_07	euxassay_006341_08	euxassay_006341_09

euxassay_006341_10	euxassay_006341_11	euxassay_006341_12	euxassay_006341_13	euxassay_006341_14

euxassay_006341_15	euxassay_006341_16	euxassay_006341_17	euxassay_006341_18	euxassay_006341_19

euxassay_006341_20	euxassay_006341_21	euxassay_006341_22	euxassay_006341_23	euxassay_006341_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19690_wholemount_strong.wlz

19690_wholemount_moderate.wlz

19690_wholemount_weak.wlz

19690_wholemount_possible.wlz

19690_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19690_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand mesenchyme

moderate

regional

moderate expression: see section 01 02

foot mesenchyme

moderate

regional

moderate expression: see section 01 07 21

lower leg mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 21

upper leg mesenchyme

moderate

regional

moderate expression: see section 03 04 21

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 21 23 24 weak expression: see section 20 22

brain

moderate

regional

moderate expression: see section 01 02 05 06 07 08 09 10 11 12 13 14 15 16 weak expression: see section 03 04 17 18

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 06 weak expression: see section 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 05 06 07 08 weak expression: see section 03 04 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 06 weak expression: see section 17

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05

trigeminal v nerve

moderate

regional

moderate expression: see section 09 10 weak expression: see section 17

spinal cord

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 07 09 weak expression: see section 11 12 13 14

cervical ganglion

moderate

regional

moderate expression: see section 07 09 weak expression: see section 13 14 15 16

thoracic ganglion

weak

regional

weak expression: see section 09 10 11

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 08 12 13 weak expression: see section 14

tongue muscle

moderate

regional

moderate expression: see section 13 weak expression: see section 11 12 14 15

midgut

strong

regional

strong expression: see section 21 22 23 24 moderate expression: see section 16 17 18 19 weak expression: see section 10 11 12 13 14 15 20

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 12 13 14 15 16 21 22 23 24 weak expression: see section 11 17 18 19 20

bladder

moderate

regional

moderate expression: see section 13 weak expression: see section 12

lung

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 21 weak expression: see section 17 18 19 20

tail paraxial mesenchyme

moderate

regional

moderate expression: see section 08 09 10 13 14 16 17 weak expression: see section 11 12 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9699
Entity Detected:	BC061237, cDNA sequence BC061237 ( MGI:2682320)
Sequence:	sense strand is shown >T9699 AGTGATGTCACCAGTGTTGTGGTAACAAGAGTTGCCTGTGAGGTATTCCATCAGATCCTAATGGGTTCCC AAGCAGGGTCATTTAGGAAGGCATCACCCCAAACTCCCAACATCAATGAGAACGAGAAAAGAATCAAGAG GTTGGAGAAGCTCAAAAGAGATCTCCAGAACATTAAAAATGAGAGAGACGAACTCCAGGGAATCCTGGCC AAGTACAAGGATTTGAATGACAGGATCAACTTTGAGACATTCATGCTTGAGATGCAACATAACCAGGTGA TGACTGACCTGAAGAGAATGCCCCAGGACATCAGTGAGGCCTTGTACAAGTACAAGCAGTTGACCACAGA AAATCAGTTCTACTGCTGCAGGAACTGCCACCTCCTGATTGAATCTAAGCTCATCCAGCACAAAGTCAGG ATATTGTGGAAGGAGAACAGACAGCTGCTAAGGGAGCAGATTGCATCGGAAGAATGCAACATAAAAACAA AAATACTATGTAAAGAAGGCAGCCAGAAGATCAAAGACAAATATAGCAGGCAATCAGCAGGAAAACAAAT AATTGTGGTTCCTGGAAGTAAGGCGGAGGCTGCTATGTCAAATCCATGTGCCTGAGAGTCAAAAAGGCTG GCTAAGGCTTTGTGTTCCTTCTCTGCACTTGGTGAATAGTGATTGCCCACTGGAAGAGCAACAACAGGAC CAAAAACAACAACTTAAATAATGGACTTTTTGTGAATCTAAGGGGAATGTCTCTCTTAAAAAGAAAAAAA ATGTCTTCTTATAATGGAACTGAATATGTCTGCTATGTATTAGTCAGTTTGTTTCACTAGCATTAGAAAG GTATGAGGAAGAATTTATTAACATCTCATCACCAATAAAAATTAAGGTTTTAAAAGTTAAAAAAAAAAAA AAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6705671 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6705671 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19687 same embryo
	EMAGE:19688 same embryo
	EMAGE:19689 same embryo
	EMAGE:19691 same embryo
	EurExpress:euxassay_006341 same experiment
	MGI:4823433 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source