EMAGE mouse gene expression spatial database

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EMAGE:19697

Ecel1 endothelin converting enzyme-like 1 ( MGI:1343461)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006160. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006160_01	euxassay_006160_02	euxassay_006160_03	euxassay_006160_04

euxassay_006160_05	euxassay_006160_06	euxassay_006160_07	euxassay_006160_08	euxassay_006160_09

euxassay_006160_10	euxassay_006160_11	euxassay_006160_12	euxassay_006160_13	euxassay_006160_14

euxassay_006160_15	euxassay_006160_16	euxassay_006160_17	euxassay_006160_18	euxassay_006160_19

euxassay_006160_20	euxassay_006160_21	euxassay_006160_22	euxassay_006160_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19697_wholemount_strong.wlz

19697_wholemount_moderate.wlz

19697_wholemount_weak.wlz

19697_wholemount_possible.wlz

19697_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19697_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8860
Entity Detected:	Ecel1, endothelin converting enzyme-like 1 ( MGI:1343461)
Sequence:	sense strand is shown >T8860 CGACCAAACAAGGGGTCTGCAGCCAAAGCCTCGGCACAATGGAGGCCCCGTATTCCATGACAGCGCACTA CGACGAGTTCCAGGAAGTCAAGTATGTGAGCCGGTGTGGCACCGGGGGCGCTCGAGGGACCTCACTGCCT CCAGGCTTCCCGAGGGGCTCAGGGCGCAGTGCTAGCGGGTCCAGGTCTGGGCTGCCCCGCTGGAATCGAC GCGAGGTGTGCCTGCTGTCGGGGTTGGTGTTTGCTGCCGGCCTCTGCGCCATCCTGGCGGCCATGCTGGC GCTCAAATACCTGGGCCCGGGAGCGGCGGGCGGTGGCGGAGCCTGTCCCGAGGGCTGCCCCGAGCGCAAG GCCTTTGCACGCGCCGCCCGCTTTCTGTCTGCCAACCTGGACGCCAGCATCGACCCGTGCCAGGACTTCT ACTCGTTCGCGTGCGGAGGCTGGCTGCGGCGCCACGCTATCCCCGACGACAAGCTCACCTACGGCACGAT CGCGGCCATCGGAGAGCAGAACGAGGAGCGTCTGCGGCGCCTGCTGGCGAGACCCACCGGAGGCCCCGGC GGCGCTGCGCAGCGCAAGGTGCGCGCCTTCTTCCGCTCGTGCCTCGACATGCGCGAGATCGAGAGGCTCG GGCCGCGGCCCATGCTCGAGGTCATCGAGGACTGCGGCGGCTGGGACCTGGGCGGCGCGGCCGATCGCCC CGGGGCGGCTCGCTGGGACCTCAACCGACTGCTGTACAAGGCACAGGGTGTGTACAGCGCGGCCGCGCTC TTCTCGCTTACAGTCAGCCTGGACGACAGGAACTCCTCGCGCTACGTCATCCGCATTGACCAGGATGGGC TCACCCTGCCAGAGAGAACCCTGTACCTAGCTCAAGACGAGGAGAGTGAGAAGATCTTGGCTGCATACAG GGTGTTCATGCAGCGTCTACTCAGACTACTGGGAGCGGACGCTGTGGAGCAGAAGGCGCAGGAAATCCTT CAGCTGGAACAGAGGCTGGCCAATATCTCTGTGTCAGAATATGATGATCTCCGTCGTGATGTCAGCTCCG CGTACAACAAAGTGACTCTGGGGCAGCTGCAGAAGATCATCCCCCATTTGCAGTGGAAGTGGCTGCTGGA TCAGATCTTCCAGGAAGACTTCTCGGAGGAGGAGGAGGTGGTGCTGCTGGCCACAGACTACATGCAGCAG GTGTCCCAGCTCATCCGCTCCACACCTCGAAGGATCCTGCACAACTACCTAGTGTGGCGTGTGGTGGTGG TTCTGAGTGAGCACCTGTCCTCACCATTCCGAGAGGCACTGCATGAGCTGGCCAAAGAGATGGAGGGCAA TGACAAGCCCCAGGAGTTGGCCCGAGTCTGCCTGGGCCAGGCCAACCGCCACTTCGGCATGGCTCTTGGT GCCCTCTTTGTACATGAACACTTCTCAGCTGCCAGTAAAGCCAAGGTGCAGCAGCTAGTGGAAGATATCA AGTACATCTTGGGCCAGCGCCTGGAGGAGCTGGACTGGATGGATGCCCAGACCAAGGCAGCTGCTCGGGC CAAGCTCCAGTACATGATGGTCATGGTTGGCTATCCAGACTTCCTGCTGAAACCTGAGGCTGTAGACAAA GAATATGAGTTCGAGGTCCACGAGAAGACCTACTTCAAGAACATTTTGAACAGCATCCGATTCAGTATCC AGTTGTCCGTCAAAAAGATTCGACAGGAGGTGGACAAATCCTCGTGGCTCCTCCCACCACAGGCGCTCAA TGCCTACTATCTGCCCAACAAGAATCAAATGGTCTTCCCAGCTGGCATCCTGCAGCCCACCCTGTATGAC CCAGACTTCCCACAGTCTCTGAACTACGGGGGTATTGGCACCATCATTGGGCACGAACTGACCCATGGCT ATGACGACTGGGGGGGACAGTATGACCGCTCAGGAAACCTGTTGCACTGGTGGACTGAGACCTCCTACAG CCACTTTCTGCGCAAGGCCGAGTGCATCGTTCGCCTCTATGACAACTTCACCGTCTACAACCAGCGCGTG AATGGGAAACACACACTTGGCGAGAACATCGCAGACATGGGAGGCCTCAAGCTGGCCTACTATGCCTATC AGAAGTGGGTCCGGGAGCATGGCCCGGAGCACCCGCTGCACCGGCTCAAGTACACACACAACCAGCTTTT CTTCATTGCCTTTGCACAGAACTGGTGCATCAAGCGACGGTCACAGTCCATCTACCTGCAGGTGCTGACA GACAAGCATGCACCTGAGCACTACCGGGTCCTGGGCAGCGTATCCCAGTTTGAGGAATTCGGCCGGGCCT TCCACTGTCCCAAGGACTCTCCCATGAACCCCGTCCATAAGTGCTCTGTGTGGTGAGCCTGGCCGTCTGC CTGTCTACCTGCACGTCCCCACTGCCCTGCACGGATCACCTCTACCAGCTGTGGGGCAACATGAGCCCCA TGCTTCCTGCTCCGGGTCTATCTTCCTTCCCAACCTTTATGTGATTGTCTTCCCGCCCCCTGCAGTCCTT CACTTAAGCGAGGGCCTGGAGCAGGGGTAAGGCTAGGTTCTGGGGGACACTCGAGGGGGAATTATGAGGT CCTCACACCCGGCTCTAGGCAGCTGGACCCCCCTACCGGGGCTGGATAGGTCCCACTGATGTGTTCTTGC TGATTAGTCTGGTCAATAAAGCTGCTGTACTATATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6413731 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6413731 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19700 same embryo
	EMAGE:19699 same embryo
	EMAGE:19698 same embryo
	EurExpress:euxassay_006160 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source