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EMAGE:197

Fgf3 fibroblast growth factor 3 ( MGI:95517)
TS14 (9 dpc)
in situ hybridisation

Data Images
EMAGE:197 EMAGE:197
Figure 3A of Mark et al., 1993 [PMID:8287791] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright. Figure 3B of Mark et al., 1993 [PMID:8287791] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: no stars
Notes:
Corresponding bright and darkfield images are shown below. Abbreviations used: OP - optic pit, R - rhombomere.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
rhombomere 05
detected detected
rhombomere 06
detected detected
regionalExpression detected in the rostral portion of R6.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:10698
Entity Detected:Fgf3, fibroblast growth factor 3 ( MGI:95517)
Notes:The Fgf3 (int2) probe used in this study by Mark et al., 1993 [PMID:8287791] , is indicated as being originally described by Wilkinson et al., 1988 [PMID:3293998] . Wilkinson used two non-overlapping probes that gave 'identical' results: int-2f ("a 532 bp SacI-EcoRI fragment" covering 3' of exon 3 and 3' UTR) and int-2g ("a 496 bp EcoRI-ScaI fragment" immediately adjacent to int2f covering 3' UTR). Unable to ascertain which is used here. Mark et al. hydrolysed the probe to "an average of 150 nucleotides by limited alkaline hydrolysis".
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Age:9 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:autoradiography
General Information
Authors:Mark et al., 1993 [PMID:8287791] . Indexed by GXD.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:8287791] Mark M, Lufkin T, Vonesch JL, Ruberte E, Olivo JC, Dolle P, Gorry P, Lumsden A, Chambon P 1993 Two rhombomeres are altered in Hoxa-1 mutant mice. Development (119):319-38
 [ PMID:3293998] Wilkinson DG, Peters G, Dickson C, McMahon AP 1988 Expression of the FGF-related proto-oncogene int-2 during gastrulation and neurulation in the mouse. EMBO J (7):691-5
Links:MGI:1343204 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI