EMAGE mouse gene expression spatial database

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EMAGE:19729

Myo1b myosin IB ( MGI:107752)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006192. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006192_01	euxassay_006192_02	euxassay_006192_03	euxassay_006192_04

euxassay_006192_05	euxassay_006192_06	euxassay_006192_07	euxassay_006192_08	euxassay_006192_09

euxassay_006192_10	euxassay_006192_11	euxassay_006192_12	euxassay_006192_13	euxassay_006192_14

euxassay_006192_15	euxassay_006192_16	euxassay_006192_17	euxassay_006192_18	euxassay_006192_19

euxassay_006192_20	euxassay_006192_21	euxassay_006192_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19729_wholemount_strong.wlz

19729_wholemount_moderate.wlz

19729_wholemount_weak.wlz

19729_wholemount_possible.wlz

19729_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19729_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 12 13 14

telencephalon ventricular layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

medulla oblongata basal plate ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13

pons ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 15 16 17

midbrain ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

spinal cord mantle layer

moderate

regional

moderate expression: see section 10 11 12

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 11 17 weak expression: see section 10 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T443
Entity Detected:	Myo1b, myosin IB ( MGI:107752)
Sequence:	sense strand is shown >T443 AGNCTGTTGGCCTACTGGTGTTAGCGTCCTCTTGGCACAATTAAAAGTATTTGACTAATGAGTTTTAATT ATCAGAATAGTTCATTCCTAAGTCCACATTCTGTCCTGGGCAGAATTCTAGAAATTAACAGTGTCTGTCT CCATGGTTTATGTGTTTTCTCCTTAGTTACCTAGACTTGTGTACCCTAAACCCACATATTACCCATAAAT CACTAGTACATGTCAGCACAGGAGGGGAGCTTACAGTCAGTTATCAGATATTTATTGAATGCCCACTTTT TGCTGGGCCCTGTGCTGAACTTATTCGGAGATTTCTATTTTTAAAGCTGTTGCCTTCTGGCTGATTTGTG CTGTGGAAGCAACAGCTAAGAAAGTTGAAGAGATTGGGTCAAATGAGACTATAAATGACGGCCACTGGTA GGAACCCAGTAGTGTGGCGTAAACAGTACACTCACTGAGAAGTAGAGGTCCTGGGTGGATTGGAGATAGG TAATTGGGTAGAATTCTCCTGAGAGGTTTGG
Notes:	The probe template was PCR amplified from IMAGE:1431692 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1431692 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19731 same embryo
	EMAGE:19730 same embryo
	EurExpress:euxassay_006192 same experiment
	MGI:4826560 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source