EMAGE mouse gene expression spatial database

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EMAGE:19735

Amy1 amylase 1, salivary ( MGI:88019)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006206. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006206_01	euxassay_006206_02	euxassay_006206_03	euxassay_006206_04

euxassay_006206_05	euxassay_006206_06	euxassay_006206_07	euxassay_006206_08	euxassay_006206_09

euxassay_006206_10	euxassay_006206_11	euxassay_006206_12	euxassay_006206_13	euxassay_006206_14

euxassay_006206_15	euxassay_006206_16	euxassay_006206_17	euxassay_006206_18	euxassay_006206_19

euxassay_006206_20	euxassay_006206_21	euxassay_006206_22	euxassay_006206_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19735_wholemount_strong.wlz

19735_wholemount_moderate.wlz

19735_wholemount_weak.wlz

19735_wholemount_possible.wlz

19735_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19735_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

facial vii ganglion

moderate

regional

moderate expression: see section 04 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 17 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 07 16

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 07 16 17 18 19

spinal cord

moderate

regional

moderate expression: see section 07

dorsal root ganglion

weak

regional

weak expression: see section 05 06 07 08 09 10 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T628
Entity Detected:	Amy1, amylase 1, salivary ( MGI:88019)
Sequence:	sense strand is shown >T628 TCCTCNAGNCTGTTGGCCTACTGGAGTCCATCATAGGTCACTGTGGAGCTCAGATCACAGTGCTGACAGA ATCCATATTTGGAGAATTACATAAGGTTTGAAAGAGAGGATAGTGAAAGGATACGAATTCCTAAAAACGT TTAATCTGGCCTTTTGTTTGAACGAAAGAGAAATTGAAACCAAATGAAATAAATTACTTGTTAGAAAGAA TACTGCCAACAGCATAGCAAAATGAAATTCTTCCTGCTGCTTTCCCTCATTGGATTCTGCTGGGCCCAAT ATGACCCACATACTCAATATGGACGAACTGCTATTGTCCACCTGTTTGAGTGGCGCTGGGTTGATATTGC TAAGGAATGTGAGAGATACTTAGCTCCTAATGGATTTGCAGGTGTGCAGGTCTCTCCACCCAATGAAAAC ATCGTAGTCCACAGCCCTTCAAGACCATGGTGGGAAAGATATCAACCAATTAGCTACAAAATATGTTCCA GGTCTGGAAATGAAGATGAATTCAGGGACATGGTGAACAGGTGCAACAATGTTGGTGTCCGTATT
Notes:	The probe template was PCR amplified from IMAGE:1886328 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1886328 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19734 same embryo
	EMAGE:19737 same embryo
	EMAGE:19736 same embryo
	EMAGE:19732 same embryo
	EMAGE:19733 same embryo
	EurExpress:euxassay_006206 same experiment
	MGI:4823117 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source