EMAGE mouse gene expression spatial database

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EMAGE:19750

March1 membrane-associated ring finger (C3HC4) 1 ( MGI:1920175)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006253. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006253_01	euxassay_006253_02	euxassay_006253_03	euxassay_006253_04

euxassay_006253_05	euxassay_006253_06	euxassay_006253_07	euxassay_006253_08	euxassay_006253_09

euxassay_006253_10	euxassay_006253_11	euxassay_006253_12	euxassay_006253_13	euxassay_006253_14

euxassay_006253_15	euxassay_006253_16	euxassay_006253_17	euxassay_006253_18	euxassay_006253_19

euxassay_006253_20	euxassay_006253_21	euxassay_006253_22	euxassay_006253_23	euxassay_006253_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19750_wholemount_strong.wlz

19750_wholemount_moderate.wlz

19750_wholemount_weak.wlz

19750_wholemount_possible.wlz

19750_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19750_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

strong

homogeneous

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

adenohypophysis

moderate

regional

moderate expression: see section 12 13 weak expression: see section 10 11 14

cerebral cortex mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 20 21

telencephalon mantle layer

strong

regional

strong expression: see section 02 03 04 06 07 08 09 10 13 14 15 18 19 20 21 22 23 24 moderate expression: see section 11 12

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 10 11 12 16 17 18

hindbrain

strong

homogeneous

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain

strong

homogeneous

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 17 weak expression: see section 18

spinal cord

strong

homogeneous

strong expression: see section 09 10 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T723
Entity Detected:	March1, membrane-associated ring finger (C3HC4) 1 ( MGI:1920175)
Sequence:	sense strand is shown >T723 TCCTCGAGNCTGTTGGCCTACTGGAGATTCATGAACCTCACTATGAGCAACATGACCAGCAGCCACATTT GCTGTAATTTTTTAAATATGTGGAAAAAAAGCAAGATATCAACCATGTATTACCTTAACCAAGATGCCAA ATTATCTAACTTGTTTCTCCAGGCAAGCAGCCCAACGACAGGGACAGCTCCCAGGAGCCAGTCAAGGTTG TCTGTCTGTCCATCTACTCAGGACATCTGCAGGATCTGTCACTGCGAAGGAGATGAAGAGAGCCCACTCA TCACACCCTGTCGCTGCACAGGAACCTTGCGCTTTGTCCACCAGTCCTGCCTCCACCAATGGATCAAGAG CTCAGACACACGATGCTGTGAGCTCTGCAAGTATGACTTCATAATGGAGACCAAGCTCAAGCCCCTTCGG AAGTGGGAAAAGCTCCAGATGACCACGAGCGAAAGGAGGAAAATATTCTGCTCTGTCACGTTCCACGTCA TCGCCGTCACCTGTGTGGTGTGGTCCTTGTATGTGTTGATAGATCGGACAGCGGAG
Notes:	The probe template was PCR amplified from IMAGE:1890205 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1890205 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19751 same embryo
	EMAGE:19749 same embryo
	EMAGE:19753 same embryo
	EMAGE:19752 same embryo
	EurExpress:euxassay_006253 same experiment
	MGI:4826101 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source