EMAGE mouse gene expression spatial database

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EMAGE:19752

Sik1 salt inducible kinase 1 ( MGI:104754)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006258. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006258_01	euxassay_006258_02	euxassay_006258_03	euxassay_006258_04

euxassay_006258_05	euxassay_006258_06	euxassay_006258_07	euxassay_006258_08	euxassay_006258_09

euxassay_006258_10	euxassay_006258_11	euxassay_006258_12	euxassay_006258_13	euxassay_006258_14

euxassay_006258_15	euxassay_006258_16	euxassay_006258_17	euxassay_006258_18	euxassay_006258_19

euxassay_006258_20	euxassay_006258_21	euxassay_006258_22	euxassay_006258_23	euxassay_006258_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19752_wholemount_strong.wlz

19752_wholemount_moderate.wlz

19752_wholemount_weak.wlz

19752_wholemount_possible.wlz

19752_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19752_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diaphragm

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 16 17 18 19 20 21 22 23 24

head mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 16 17 18 19 20 21 22 23 24

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 22 23 24

thymus primordium

weak

regional

weak expression: see section 10 11 12 13 14

epidermis

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

epithalamus mantle layer

moderate

regional

moderate expression: see section 13 15 weak expression: see section 12

cerebral cortex mantle layer

moderate

regional

moderate expression: see section 17

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 16 18 19 20 21 22 23 24 weak expression: see section 02 03

rest of cerebellum mantle layer

moderate

regional

moderate expression: see section 10 11 15 16

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 03 04 22 23 24

naris

moderate

regional

moderate expression: see section 10 13 14

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 06 07 08 09 16 17 18

nasal cavity respiratory epithelium

moderate

regional

moderate expression: see section 10 11 14 15

esophagus

strong

regional

strong expression: see section 11 12

tongue muscle

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14

urethra of male

strong

regional

strong expression: see section 13

trachea

weak

regional

weak expression: see section 12 13

tail mesenchyme

moderate

regional

moderate expression: see section 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T727
Entity Detected:	Sik1, salt inducible kinase 1 ( MGI:104754)
Sequence:	sense strand is shown >T727 TCTCGAGNCTGTTGGCCTACTGGAATTGTCTCCTCCTCTGCNCGGCGAGTCCCTCGGAAGGGACCAGCTC CGACAGCTGCCTCCCCTTCTCTGCAAGTGAAGGTCCTGCAGGGCTTGGCAGTGGCCTGGCCACCCCAGGG CTGCTGGGCACCAGCTCTCCAGTCAGATTGGCCTCGCCCTTCCTGGGATCACAGTCAGCCACCCCTGTGC TCCAGACTCAGGCAGGTCTGGGCACAGCCGTCTTACCTCCCGTCAGCTTCCAGGAGGGACGGAGAGCGTC TGATACCTCTCTCACTCAGGGGCTGAAGGCCTTCCGGCAGCAGCTGAGGAAAAATGCGAGGACCAAGGGG TTCCTGGGACTGAACAAGATCAAAGGGTTGGCTCGCCAGGTGTGCCAGTCCTCCGTCCGAACTCCCCGGG GAGGGATGAGTACCTTCCACACCCCAGCCCCAAGCTCCGGCCTGCAAGGCTGCACGACCAGCAATCGGGA GGGCAGGAGCCTACTTGAAGAGGTCCTGCACCAGCAGAGGCTGCTCCAGT
Notes:	The probe template was PCR amplified from IMAGE:1890289 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1890289 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19751 same embryo
	EMAGE:19749 same embryo
	EMAGE:19753 same embryo
	EMAGE:19750 same embryo
	EurExpress:euxassay_006258 same experiment
	MGI:4828072 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source