EMAGE mouse gene expression spatial database

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EMAGE:19757

Aff3 AF4/FMR2 family, member 3 ( MGI:106927)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006287. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006287_01	euxassay_006287_02	euxassay_006287_03	euxassay_006287_04

euxassay_006287_05	euxassay_006287_06	euxassay_006287_07	euxassay_006287_08	euxassay_006287_09

euxassay_006287_10	euxassay_006287_11	euxassay_006287_12	euxassay_006287_13	euxassay_006287_14

euxassay_006287_15	euxassay_006287_16	euxassay_006287_17	euxassay_006287_18	euxassay_006287_19

euxassay_006287_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19757_wholemount_strong.wlz

19757_wholemount_moderate.wlz

19757_wholemount_weak.wlz

19757_wholemount_possible.wlz

19757_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19757_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8467
Entity Detected:	Aff3, AF4/FMR2 family, member 3 ( MGI:106927)
Sequence:	sense strand is shown >T8467 CGGGAAGTTTGTAGTGGAAACCCAGAGAGGCCTTTCCAGTATGAAATAAAAAGAAAGGAGACTGGTTTCC AGGGACTGGGCAGCTGAGGGCTAGTGGGATACCCCTCGTCCTCCCAGCCCCCAGGCTAAACTGCAGCTGA AAGCAGGTCTTGGAAGACGCTTCCTTCCCGCCTCCTCTTTATTTCACTTAAGTTACTGGCTCCTCAGGCC GGTGGCCCCAGCGTGTCTGGGGCCACTGGCTCCTCAGGCCGGTGGCAGTTGCCCCAGACACGCTGGGGGC GAGCGTGGTGGGACCCCTAGAGCCCCCCAAAACCAGACCCTGCGGTAACAACAGGACAGGCCACCGCAAG GAGCTGCGCTCCTCCGTGACCTGTGAGAAGCGGCGGCGGACACAGCGGCGGCGGCGGACACGGCGGCGGC GGCGCTGACACCTCCCACCATGGACAGCTTCGACTTAGCCCTGCTCCAGGAATGGGACCTCGAGTCGCTG TGGGGTGAAGATATCCTAAGCCAGAGGAATGATTCTCTAGTTCTGGAAGTTCAGGCATCAGCCAGCAGAT GCAGGAGTGTCTATGAACCGGACAGGAATGCGTTACGGAGGAAAGAGCGAGAAAGAAGAAGTCAAGAAAC ACAGCAGGACAGTGGCAGCTTTAACTCCGGTTACTCGCTCTTCAGCGAACCCTACAAGACGAACAAGGGG GATGAACTCTCCAACCGGATCCAGAATACATTAGGAAATTATGATGAGATGAAAGACTTTTTAACTGATA GATCCAACCAGAGTCATCTTGTTGGCGTCCCCAAGCCAGGTGTCCCTCAGACTCCCGTGAACAAGATAGA TGAGCATTTTGGGGCAGAGTCGAGAGCCCAGCCCCAGCCCTCAACCGTCTGCAGCACTGCGTCCTCTACA CCTGCAGCTGTCCCTGTGCAGCAGGGCAAGAGAGGTGCCATGGGCTGGCAGAAGGCTGGGCACCCACCGT CCGATGGCCAACAGAGAGCAGCGCAACAGGGCTCTCTCAGGACCTTGCTTGGAGATGGTGTTGGCAGACA GCAGACAAGGGCCAAGCAAGTGTGCAATATGGAGACGGGGCTTCAGACCCAGGAGAGGCCACCTGCAATG GCAGCCAAGCACGGCGGCAGTGGACACTGTGTTCAGAACTTTCCCCCATCCCTGGCTTCAAAACCCAGCC TGGTGCAGCAGAAACCAACTGCCTACGTGCGGCCAATGGATGGCCAGGACCAAGCTCCTGACGAATCTCC TAAGCTGAAGTCATCCACAGAAACTGCTGTGCACTGCACAGCCTACAGAGGGGTCCCGGCCAACAAACCA GAGTCAGCCCGAGCCAAGGCCAAGCTTGCCAAGTTCAGCATCCCCAAGCAGGCAGAGGAGAGTAGATCTG GAGAAAACAACAGCTGTGTGGAAGAAATAATTCGGGAGATGACCTGGCTTCCTCCACTTTCTGCTATTCA AGCTCCTGCCAAAGTGGAACCAAGCAAGTTCCCATTCCCAAATAAGGACTCGCAACTTGTATCCTCTGGA CACAGTAATCCAAAGAAAGCTGATGCGGAGCCAGGGAGTCCAGACAATGGCGCATCGAACACTTCAACTC TGGAAGACGACCTTAAGCTAAGCAGTGATGACGAAGAGGGTGAGCAGGCAGCCCAGAGAACAGCTCTCCG CGCTCTAGCTGACAGCTCCGTGGTTCAGCAGACCAACTGTAGAGGTTCGGCGCCATCCAGCAAGGGCGGC GGCAGCAGCAGCAGCAGCGGCGGCAGCAGCTCCTCCAGCGACTCAGAGAGCACTTCCGGCTCCGACTCCG AGACGGAGAGCAGCTCCAGCTCCAGCGAGAGCGAGGGCAGCAAGCCTCCGCACTGCTCCAGCCCCGAGGC CGAACCTGCGTCCTCTAACAAGTGGCAGCTGGATAAATGGCTAAACAAAGTTAATCCTCACAAGCCGCCA ATTCTGATCCAAAATGAAAGCCACGGCCCGGAAAGGAATCAATACTACACGCCGCCGGTGAAAGATGAAG GGCAAGACTGCGGGAAACTTCCTGAGATCTGCCAAGCCAGCCTGAGGGACAAGGAGCTCAAGACCACCTG CAAGGAGGAGCAGAGGCCGAGGACCTGACTTCTTCCAGCCGACCTCACGGCAACGGGTTGTTGACTTCAG CGTCTTCCAACAAAGAGCCAAAGGCTGAGAGTCAGCTGCAGACGATTGCTGGGGACCTCACGAAGGCATC TCACAACAGTTCTGAAAATGGCACCCTCCATAGCAAGTCACGGCCCCAGACGGAGCCATGGTCTCCAGGT TCCAATGGCCACAGGGACTGCAAGAGACAGAAACTGATCTTCGATGACATGCCTCGAAGTGCAGATTATT TTATGCGAGAAGCCAAGCGGATGAAACATAAAGCAGACGCCATGGTGGAGAAGTTCGGGAAGGCCCTGAA CTATGCCGAAGCTGCCTTGTCCTTCATCGAGTGCGGAAACGCAATGGAACAAGGGCCAATGGAATCCAAG TCTCCCTACACCATGTACTCGGAGACAGTGGAGCTCATCAGGTATGCTATGAGACTAAAAACCCACTCAG GCCCCAATGCCACACCAGAGGACAAACAGTTAGCTGCGCTTTGTTACCGATGCCTGGCTCTCCTATACTG GCGGATGTTTCGACTCAAAAGAGACCATGCTGTAAAATATTCAAAAGCACTTATCGACTATTTCAAGAAT TCATCGAAAGCTGCCCAAGCCCCATCCCCATGGGGGTCCAGTGGCAAGAGCACTGGAAGCCCATCCCCCA TGTCTCCCAACCCTTCTCCCGCCAGCTCCGTGGGGTCTCAGGGCAGCCTCTCCAGCTCCAGCGGCCTGTC CCCATCGACCATCGTCAGCATCCCCCAGCGCATTCACCAGATGGCGGCCAATCACGTCAGCATCACCAAC AGCATCCTCCACAGCTATGACTACTGGGAGATGGCTGATAACCTGGCCAAGGAAAACAGAGAGTTCTTCA ATGACCTGGATTTGCTCATGGGACCTGTCACCCTGCACAGCAGCATGGAACACCTGGTCCAGTACTCCCA GCAAGGCCTGCATTGGCTTCGGAGCAGCGTACACCTGTCCTAGGGACCAAGCCTTTGAGCCAGACTGTGT TTTCTTCTCTTCAGTGGCTCAATGTTTCCGGACACTGTGCTACTGACATTCCCAGCCCAAACCACAGTGA ACATCTCAGAGTCTAACAACGATGTTTCTCTGGGGTGTGTGTGTTTAGATGTGGGTTCGTACAAAGCTGC CTGTGTATGTGTGTAAGATCAACAGCTCATCAAAATGTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5702935 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5702935 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19758 same embryo
	EMAGE:19756 same embryo
	EMAGE:19755 same embryo
	EMAGE:19754 same embryo
	EMAGE:19759 same embryo
	EurExpress:euxassay_006287 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source