EMAGE mouse gene expression spatial database

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EMAGE:19772

Igf2bp3 insulin-like growth factor 2 mRNA binding protein 3 ( MGI:1890359)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006316. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006316_01	euxassay_006316_02	euxassay_006316_03	euxassay_006316_04

euxassay_006316_05	euxassay_006316_06	euxassay_006316_07	euxassay_006316_08	euxassay_006316_09

euxassay_006316_10	euxassay_006316_11	euxassay_006316_12	euxassay_006316_13	euxassay_006316_14

euxassay_006316_15	euxassay_006316_16	euxassay_006316_17	euxassay_006316_18	euxassay_006316_19

euxassay_006316_20	euxassay_006316_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19772_wholemount_strong.wlz

19772_wholemount_moderate.wlz

19772_wholemount_weak.wlz

19772_wholemount_possible.wlz

19772_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19772_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8516
Entity Detected:	Igf2bp3, insulin-like growth factor 2 mRNA binding protein 3 ( MGI:1890359)
Sequence:	sense strand is shown >T8516 GGTTTTCGCCCGCCGCTCCCGTGCTTGGGTCACTGTGTGGGTTTGAAAGCCAAGAGGAGAGGAACAAAAT CCACCGGCTCCATCCTCCGCCGTGGGTGCTTTTTTTAATTTAGCATTTTCCTCGGGTTTTAAAACGACCC CTTTTCACAATGAACAAATTGTACATCGGGAACCTCAGCGACCACGCCGGCCCCGCGGACCTGGAAAGTG TCTTCAAGGACGCTAAGATCCCGGTGGCGGGCCCCTTCCTGGTGAAGACGGGCTACGCGTTCGTGGACTG CCCGGACGAGGGCTGGGCCCTCAAGGCCATCGAGGCGCTTTCAGGTAAAATGGAACTACATGGGAAACCG ATGGAAGTTGAGCACTCGGTCCCTAAACGGCAGAGGATTCGTAAACTTCAGATACGAAATATCCCGCCCC ACTTACAATGGGAGGTGCTGGATAGTTTACTAGTCCAGTATGGAGTGGTGGAGAGCTGTGAGCAAGTGAA CACGGATTCGGAAACGGCAGTTGTAAATGTAACCTATTCCAGTAAGGACCAAGCTAGACAAGCCTTAGAC AAACTGAATGGATTCCAGTTAGAGAACTTCACCTTGAAGGTTGCCTACATCCCAGATGAAACTGCTGCCC AGCAAAATCCCTCGCCACAGCTCCGGGGGCGCCGGGGGCCAGGGCAGCGGGGCTCATCCAGGCAGGCGTC TCCAGGATCGGTGTCCAAGCAGAAACCCTGTGACCTGCCACTGCGCCTGCTGGTTCCCACCCAGTTTGTT GGAGCCATTATAGGAAAAGAAGGTGCTACCATTCGCAACATCACAAAGCAGACCCAGTCTAAAATCGATG TCCATCGTAAGGAGAATACAGGGGCCGCGGAGAAGTCCATTACTATCCTCTCTACCCCTGAAGGCACCTC TGCAGCTTGTAAGTCTATTCTGGAGATTATGCATAAGGAAGCTCAAGATATAAAATTCACAGAGGAGATT CCCTTGAAGATCTTAGCTCACAATAACTTTGTAGGCCGTCTCATTGGTAAAGAAGGAAGAAACCTTAAAA AAATCGAGCAAGACACGGACACTAAAATCACAATATCTCCATTGCAGGAACTGACGCTGTACAATCCGGA ACGCACCATTACAGTGAAAGGCAGTGTTGAGACGTGTGCCAAGGCGGAGGAGGAAATAATGAAGAAGATC AGGGAGTCTTATGAAAATGATATTGCTTCCATGAATCTTCAAGCACATTTAATCCCTGGATTAAATCTGA ATGCCTTGGGTCTGTTCCCACCCACGTCAGGGATGCCACCTCCCACCTCAGGGCCCCCTTCAACCCTGAC TCCTCCCTACCCACAATTTGAGCAATCAGAGACGGAGACTGTGCATCTGTTTATTCCCGCCCTGTCCGTT GGCGCCATCATTGGCAAGCAGGGCCAACACATCAAACAGCTTTCTCGCTTTGCGGGAGCTTCGATTAAGA TCGCTCCAGCCGAAGCACCAGATGCTAAAGTGCGGATGGTGATTATCACTGGACCACCAGAGGCTCAGTT CAAGGCTCAGGGAAGAATTTATGGAAAAATTAAAGAAGAAAACTTTGTTAGTCCTAAAGAAGAGGTGAAA CTTGAAGCTCACATCAGAGTGCCGTCCTTTGCTGCTGGCAGAGTTATTGGGAAAGGAGGCAAAACGGTGA ATGAGCTCCAGAGTTTATCAAGTGCTGAAGTTGTCGTCCCCCGTGACCAGACACCTGATGAGAATGATCA AGTAGTTGTCAAAATAACTGGCCACTTCTATGCTTGCCAGGTTGCCCAGAGGAAAATTCAGGAAATTCTG ACTCAGGTAAAGCAGCACCAGCAGCAGAAAGCTCTGCAGAGTGGACCACCTCAGTCAAGGCGGAAGTAAA GGCTCAGGAGACAGCCACCACAGAGGCAGATGCCAAACCAAAGACAGATTTGCTTAACCAACAGACGGGT GCTGACCCATCCAGAACCACGTGCACGAGTTTTTACCTAGCTGGTTGTTTCCGAGGACCAGGCACTCGAT CCTGTCTCTGTGAGAATGTATACTTTATGCTCTCTGAAATATATGACACCCAGCTTTAAAAAAAAAAAAA AAAAAAAAAAAAAAGTGGGGGTAAGGAAAGAGAACTCTGCACTTCCCTTGTGATATCAAAGTATAACAAA CGTGCTAATTTTTCTTAATATCCCTCTATAATGCCAGAAATTGGCATAAATGGTACATTCACTAAATTTA TCAAATAAATTTAAAACTAGATTGTTCCTAAGTCCAATTGTTGAAATTGGATCAAAGTAGAATTGTCAGA GGAACACAACATTAAGCCATTAGAATAGAAGAGCTGTTGTCTCCTGTTTCTCTAACACTAACATGGATAA CCTAAGGGAAGTGCCAGCCGCTGTTGGCAGGGTATTAAACGTGCATTTTTACTCAACTACCTCAGGTATT CAGTAATACAGTTAAAAGCAAAATTATTCCTTTTTTGAAAATTTTATATACTTTATAAAAACTCCAACCG TTTTTTTAAAAAAAATAAAATTTAACAGTTATCAGCTGATAGGCAAGTCATTGAAAAAGTAATTTTACGT CTGGCTGGTGAGAGTAAAGTTGGAAAAATAATTTTGCATTTTTTGAGCCTTTGAAAGTTGGAAAGTCCAC TAACTGTTCAAAGATAAGGAGCAGTGCCTATTCTCAAGAGCAGCCATGCCATGTACTCCTTTGTTCCTAT CTGGGAAGGTTGTTGACGGTACTGACCAAGCAGGCAGGGGCTGGGACGGCCAGTGTACAGGACAGCTAGG GTAAAGGACAGCTAGTGTACAGGGCTCCAGGAGACTTGGGGATTTGTGTTTAGCTATGTGTGGGAATTTG CAAATGCTTTGTGCTTTGACTGTCACCACGAAAAGGAAGAAATTCATGTCAGTGAAGAAATGCAGAATTT AAACAAAATCAGCTTGAGTTTGTTTTACCAGATGCTCAGCTTGCTAACACACTTGAGATCAGACCATACT CTGAGCAGACATGGAAAGGATTGCTTTGTGAAGACCAGGGGGAGTCAGGTGGTATGACAGGGCCGAAGGG CAACAAAATCTCCATTTCCTTGTGCCTGGCACTGTCTCGAGCCTAAGCTATTTTAAAGATGTTTTAAACA GTGGAAGAATTAAGTCCACCAAAAGTCTTCCATGAAATGTAGCAGAGTTTCTGTCTGCCCTGCATTACCT GCCAGCGAGTCAAGGTAGGGGATCCAGCAAACGGTTTTGGGAATTCAGACTGTGGAAACATCGGACTTCC CTTTGCCCTTCCTCAACTTAGTGGCAAAGCCCTGTATGGATACTTGTAGGTGCGGGTGATCCTCAAGGCC ATGCTGCTTTCTGAGTTGTTCTTGTTGCTTTGTTCATAATGTGGTTACAGTCATATTACACTGACCCTGA GGGTATTATGTAACCCTTCAGAAAACCACTTTGCCTCATTGTGTTTCAGGATTAACTTCTACATGCCTTT AAGTTTTTCTGAGTATCAGCATATCATTTAGACATTGTGAAAATGAATTCCTAAACATTTTCATAAAACC GGTTAATTTTGTAGTTTTAGCCAAAAAGTGCCCCCTTTTTACAACTGAATTCATCTAGCATTTTCATATG GTGGATTTAAACTGCTTAAAAAAGAAAATGTGACCGGCTCTCCTGCTTGGGTCTCAGGGGAGATATGCTT AAGGCCAGAGCTCTTCCTCAGTATTTGATTTTTCCAGTGTTTGTTTTTTAAAAAACTGACAGGTGCTACA TTTCTATCTGTTGGTTTCAATTCTGCCATATTTCATGTCTAGCCTTTAGAGTGGCCAATCATGTTTTACT TTCATTTAAGCACTGGTAGTCTGGAGTACCTGGTACTGAGTAACTCTATAATTGTGTTTTGTGTTCACCA TATACAGATCATTTCTCATCTGTAGTGTGCCCCAAATGCAGCTTCACTTTCCAGGTACCCTGATGCAGAA TAAAGTTTTCATCATAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5706238 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5706238 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19777 same embryo
	EMAGE:19774 same embryo
	EMAGE:19776 same embryo
	EMAGE:19773 same embryo
	EMAGE:19775 same embryo
	EurExpress:euxassay_006316 same experiment
	MGI:4825537 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source