EMAGE mouse gene expression spatial database

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EMAGE:19774

Cltb clathrin, light polypeptide (Lcb) ( MGI:1921575)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006292. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006292_01	euxassay_006292_02	euxassay_006292_03	euxassay_006292_04

euxassay_006292_05	euxassay_006292_06	euxassay_006292_07	euxassay_006292_08	euxassay_006292_09

euxassay_006292_10	euxassay_006292_11	euxassay_006292_12	euxassay_006292_13	euxassay_006292_14

euxassay_006292_15	euxassay_006292_16	euxassay_006292_17	euxassay_006292_18	euxassay_006292_19

euxassay_006292_20	euxassay_006292_21	euxassay_006292_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19774_wholemount_strong.wlz

19774_wholemount_moderate.wlz

19774_wholemount_weak.wlz

19774_wholemount_possible.wlz

19774_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19774_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 12 13 16 17 18 weak expression: see section 11

medulla oblongata basal plate mantle layer

moderate

single cell

moderate expression: see section 05 06 07 08 09 10 11 12 13 14

pons mantle layer

moderate

single cell

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 05 06 17 18 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 04 05 14 15

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 15 16 17 18 19 20

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 04 15 weak expression: see section 05 06

ventral grey horn

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12

dorsal root ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8440
Entity Detected:	Cltb, clathrin, light polypeptide (Lcb) ( MGI:1921575)
Sequence:	sense strand is shown >T8440 CGCGGGGAAGATGGCTGAGGACTTCGGCTTCTTCTCATCGTCGGAGAGCGGGGCCCCGGAGGCCGCCGAG GAGGACCCAGCGGCCGCCTTCCTGGCCCAGCAGGAAAGCGAGATTGCTGGCATCGAGAATGACCCGGGTT TCGGGGCACCTGCCGCCAGCCAGGTGGCCTCTGCGCAGCCGGGACTCGCGAGCGGGGCTGGTTCAGAGGA CATGAGTACTACAGTCAATGGAGATGTGTTTCAGGAGGCTAACGGGCCTGCCGATGGCTACGCTGCGATT GCCCAGGCGGACAGGTTGACTCAGGAGCCCGAGAGCATCCGGAAGTGGAGAGAGGAGCAGAAGAAAAGGC TGCAGGAGTTAGACGCTGCCTCGAAGGTGACCGAACAGGAGTGGCGGGAGAAGGCCAAGAAAGACCTGGA GGAGTGGAACCAGCGCCAAAGTGAACAGGTTGAGAAGAACAAGATCAACAACAGGATCGCTGACAAAGCG TTCTACCAGCAGCCAGATGCTGATACCATTGGCTATGTGGCATCGGAAGAGGCTTTTGTGAAAGAATCCA AGGAGGAGACCCCGGGCACAGAGTGGGAGAAGGTAGCCCAGCTGTGTGACTTCAATCCCAAGAGCAGCAA GCAGTGTAAAGATGTGTCCCGCCTGCGCTCGGTGCTCATGTCCCTGAAGCAAACACCACTGTCCCGCTAG GTGCCCGTCACGAGAACGGCCGCGAAGCACATGGGCCTTGCTGGGGCAGAGGAGCAGCTGCTTCCGCCAG AGTGGAACTTCCTCTGGCAGCTGCCACACACAGCCTGTTCTGTTCCTCTGAATCTCTGGGAGCTAGGAAG TGGGACCCTTACCCCTTTCACCCCACAACTCCTTCCTGGTCCCCTGGCCCAGCCCCTCATGACTCCTGTC AGCCCACTCAATTGTGACTGTCCCTCCTGATGTATTTTTCTTGGCTTAAAGGGTGTGTTAACTCTTTTTA CACTTAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5699653 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5699653 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19777 same embryo
	EMAGE:19776 same embryo
	EMAGE:19772 same embryo
	EMAGE:19773 same embryo
	EMAGE:19775 same embryo
	EurExpress:euxassay_006292 same experiment
	MGI:4823939 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source