EMAGE mouse gene expression spatial database

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EMAGE:19775

Tiam1 T-cell lymphoma invasion and metastasis 1 ( MGI:103306)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006321. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006321_01	euxassay_006321_02	euxassay_006321_03	euxassay_006321_04

euxassay_006321_05	euxassay_006321_06	euxassay_006321_07	euxassay_006321_08	euxassay_006321_09

euxassay_006321_10	euxassay_006321_11	euxassay_006321_12	euxassay_006321_13	euxassay_006321_14

euxassay_006321_15	euxassay_006321_16	euxassay_006321_17	euxassay_006321_18	euxassay_006321_19

euxassay_006321_20	euxassay_006321_21	euxassay_006321_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19775_wholemount_strong.wlz

19775_wholemount_moderate.wlz

19775_wholemount_weak.wlz

19775_wholemount_possible.wlz

19775_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19775_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 07 08 09 10 11

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 14 15 16 17

epidermis

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

vibrissa

weak

regional

weak expression: see section 05 06 07 08 09 21 22

naris

weak

regional

weak expression: see section 13 14 15 16 17 18

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 12 17 18

lower jaw incisor

weak

regional

weak expression: see section 12 16

lower jaw molar

moderate

regional

moderate expression: see section 07 08 17 18

upper jaw incisor

weak

regional

weak expression: see section 12 16

upper jaw molar

moderate

regional

moderate expression: see section 07 08 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35000
Entity Detected:	Tiam1, T-cell lymphoma invasion and metastasis 1 ( MGI:103306)
Sequence:	sense strand is shown >T35000 ATGACGAATTCTGTGAGTCCCTGAAGGGCGCCTCAGTGGACAGAGACCTTCAGGAGCAGCTTCAGGCTGC CTCCATCAGTCAGCGGGCCCGAGGCCGGAGAACCCTCGATAGCCACGCCTCCCGCATGACACAGCTCAAG AAGCAAGCGGCCCTCTCGGGCATCAACGGGGGCCTGGAGAGTGCGAGCGAGGAAGTCATTTGGGTCAGGC GCGAAGACTTTGCCCCCTCCAGGAAACTGAACACGGAGATATGACTTCCTTGTCCCTGTAGAGAATATGT GTGTAGATATGATGCCGGCCCCACCCTCACTCCGCCCACCCTCCTGTGCCGTCCACGAGAATGTCCCCTT AGGTCGCACTCTTGACACAGATTTTGGCAGCTGACTTGGGTCTGAAGCCATGTAGCCACCCACCTTCATC ATTCTTAATGACACCAGAGAGAATCGTTCAGAATCCCAGACAAACAAGCTTGAGTCCTTTCCTCTGTGCC AAGGGCTTTCTTTTATTTATTCTCAATTCATCGGGGCCTGAAGATTTAGAACGAAGGACCGATAGCGCTA GAAATCAAACCGTCCCCACCCCAGCAGTCAAAACAGGGACAGGACGGTGTGCACAGATTGTCCCAGCTCC ACCTCTCTAAATCCAGTCTCGCAGCTGAGGAACAACCACCTTCAAACACACATAGAGAAACGGAACAGAT GGAAACACCTGTACAGCGTGCAGTGTTCTGACTCCTCCCCTCTTCAGCGTCCGAGAATATCTGTGTATCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 50000. Forward Primer - name:050000_F_cDNA_Tiam1, sequence:ATGACGAATTCTGTGAGTCCCT; Reverse Primer - name:050000_N_SP6_cDNA_Tiam1, sequence:AGATACACAGATATTCTCGGACG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19777 same embryo
	EMAGE:19774 same embryo
	EMAGE:19776 same embryo
	EMAGE:19772 same embryo
	EMAGE:19773 same embryo
	EurExpress:euxassay_006321 same experiment
	MGI:4828714 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source