EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19811

Cacna1a calcium channel, voltage-dependent, P/Q type, alpha 1A subunit ( MGI:109482)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006343. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006343_01	euxassay_006343_02	euxassay_006343_03	euxassay_006343_04

euxassay_006343_05	euxassay_006343_06	euxassay_006343_07	euxassay_006343_08	euxassay_006343_09

euxassay_006343_10	euxassay_006343_11	euxassay_006343_12	euxassay_006343_13	euxassay_006343_14

euxassay_006343_15	euxassay_006343_16	euxassay_006343_17	euxassay_006343_18	euxassay_006343_19

euxassay_006343_20	euxassay_006343_21	euxassay_006343_22	euxassay_006343_23	euxassay_006343_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19811_wholemount_strong.wlz

19811_wholemount_moderate.wlz

19811_wholemount_weak.wlz

19811_wholemount_possible.wlz

19811_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19811_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

central nervous system

weak

regional

weak expression: see section 07

brain

weak

regional

weak expression: see section 02 03 04 05 06 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

facial vii ganglion

moderate

regional

moderate expression: see section 03 05 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 05 06 07 08 09 16 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 07 16

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 07 08 17 18

trigeminal v nerve

moderate

regional

moderate expression: see section 09 weak expression: see section 16

spinal cord

moderate

regional

moderate expression: see section 09 weak expression: see section 08 10 11 12 13

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 10 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35005
Entity Detected:	Cacna1a, calcium channel, voltage-dependent, P/Q type, alpha 1A subunit ( MGI:109482)
Sequence:	sense strand is shown >T35005 AATGTCCTCATAGGGTTGCTTGCAAGAGACTCCTGCGGATGGACCTGCCGGTAGCAGATGACAACACAGT TCACTTCAACTCCACCCTGATGGCTCTGATCCGAACCGCCCTTGATATCAAAATTGCCAAGGGTGGAGCT GACAAGCAGCAGATGGATGCAGAGCTTCGCAAGGAGATGATGGCCATTTGGCCCAACCTGTCTCAGAAGA CCCTGGATCTGCTGGTCACACCTCACAAGTCCACGGACCTGACAGTGGGTAAGATCTACGCAGCCATGAT GATCATGGAGTATTACCGGCAGAGCAAGGCCAAGAAACTGCAGGCCATGCGAGAGGAGCAGAACCGGACA CCACTCATGTTCCAGCGCATGGAGCCTCCATCACCAACACAGGAGGGAGGACCCAGCCAGAACGCCCTTC CCTCCACTCAGCTGGACCCAGGAGGAGGCCTGATGGCTCACGAAGGCGGCATGAAGGAGAGCCCGTCCTG GGTGACCCAGCGGGCCCAAGAGATGTTCCAGAAGACTGGCACCTGGAGCCCAGAGCGAGGGCCACCCATC GACATGCCTAACAGCCAGCCCAACTCCCAGTCTGTGGAGATGCGAGAAATGGGTACTGATGGCTACTCAG ACAGCGAACACTACCTCCCCATGGAAGGCCAGACCAGGGCCGCCTCCATGCCCCGCCTCCCAGCAGAGAA CCAGAGGAGAAGGGGCCGGCCACGTGGAAATGACCTCAGTACCATCTCTGATACCAGCCCCATGAA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 50045. Forward Primer - name:050045_F_cDNA_Cacna1a, sequence:AATGTCCTCATAGGGTTGCTTG; Reverse Primer - name:050045_N_SP6_cDNA_Cacna1a, sequence:TTCATGGGGCTGGTATCAGAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19809 same embryo
	EMAGE:19806 same embryo
	EMAGE:19808 same embryo
	EMAGE:19807 same embryo
	EMAGE:19810 same embryo
	EurExpress:euxassay_006343 same experiment
	MGI:4823573 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source