EMAGE mouse gene expression spatial database

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EMAGE:19848

Cacna1h calcium channel, voltage-dependent, T type, alpha 1H subunit ( MGI:1928842)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006437. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006437_01	euxassay_006437_02	euxassay_006437_03	euxassay_006437_04

euxassay_006437_05	euxassay_006437_06	euxassay_006437_07	euxassay_006437_08	euxassay_006437_09

euxassay_006437_10	euxassay_006437_11	euxassay_006437_12	euxassay_006437_13	euxassay_006437_14

euxassay_006437_15	euxassay_006437_16	euxassay_006437_17	euxassay_006437_18	euxassay_006437_19

euxassay_006437_20	euxassay_006437_21	euxassay_006437_22	euxassay_006437_23	euxassay_006437_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19848_wholemount_strong.wlz

19848_wholemount_moderate.wlz

19848_wholemount_weak.wlz

19848_wholemount_possible.wlz

19848_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19848_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 18 19 20 21 22 23

facial vii ganglion

moderate

regional

moderate expression: see section 04 20 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 08 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 17 18 19 20 21 22

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 17 18 19

trigeminal v nerve

moderate

regional

moderate expression: see section 09 17

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 11 14 15 16 17 18

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35113
Entity Detected:	Cacna1h, calcium channel, voltage-dependent, T type, alpha 1H subunit ( MGI:1928842)
Sequence:	sense strand is shown >T35113 CTCTGAGCCTCTCACGGATACTCTACAGACAGGAGGCCATGCACGCAGAGTCCCTGGAAGGGCAGATAGA TGACGCTGGAGAAGACGGCATCCCAGACTACACGGAGCCTGCTGAAAATATCTCCATGAGCCAGGCACCT CTGGGTACCCTGCGGTCTCCTCCATGCTCCCCACGGCCTGCCAGTGTCCGTACCCGCAAGCACACTTTTG GGCAGCACTGCATCTCCAGCCGCCCTCCCACCCTGGGAGGAGATGATGCTGAAGCAGCAGATCCGGCCGA TGAGGAGGTCAGCCACATCACCAGCTCAGCCCACCCTTGGCCAGCTACAGAGCCCCACAGCCCAGAGGCC TCCCCGACAGCCTCTCCTGCAAAAGGCACAGTGGGCAGTGGGCGGGACCCACACAGGTTCTGCAGTGTAG ATGCTCAGAGCTTCCTGGACAAACCAGGTCGGCCAGATGCACAGCGGTGGTCCTCGGTGGAACTGGATAA TGGGGATGGCCACCTAGAGTCTGGGGAAGTGAGGGCCCGGGCCTCAGAGCTAGAACCAGCTCTTGGGGCA CGGAGGAAGAAGAAGATGAGCCCTCCCTGCATCTCCATTGACCCTCCCACTGAAGATGAGGGCTCCTCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 90691. Forward Primer - name:090691_F_cDNA_Cacna1h, sequence:CTCTGAGCCTCTCACGGATACT; Reverse Primer - name:090691_N_SP6_cDNA_Cacna1h, sequence:GGAGGAGCCCTCATCTTCAGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19850 same embryo
	EMAGE:19849 same embryo
	EMAGE:19851 same embryo
	EMAGE:19847 same embryo
	EurExpress:euxassay_006437 same experiment
	MGI:4823576 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source