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EMAGE:19851

Cacna1e calcium channel, voltage-dependent, R type, alpha 1E subunit ( MGI:106217)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19851 EMAGE:19851 EMAGE:19851 EMAGE:19851 EMAGE:19851
"Pseudo-wholemount" of euxassay_006436. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006436_01 euxassay_006436_02 euxassay_006436_03 euxassay_006436_04
EMAGE:19851 EMAGE:19851 EMAGE:19851 EMAGE:19851 EMAGE:19851
euxassay_006436_05 euxassay_006436_06 euxassay_006436_07 euxassay_006436_08 euxassay_006436_09
EMAGE:19851 EMAGE:19851 EMAGE:19851 EMAGE:19851 EMAGE:19851
euxassay_006436_10 euxassay_006436_11 euxassay_006436_12 euxassay_006436_13 euxassay_006436_14
EMAGE:19851 EMAGE:19851 EMAGE:19851 EMAGE:19851 EMAGE:19851
euxassay_006436_15 euxassay_006436_16 euxassay_006436_17 euxassay_006436_18 euxassay_006436_19
EMAGE:19851 EMAGE:19851 EMAGE:19851 EMAGE:19851 EMAGE:19851
euxassay_006436_20 euxassay_006436_21 euxassay_006436_22 euxassay_006436_23 euxassay_006436_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19851Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19851_wholemount_strong.wlz
19851_wholemount_moderate.wlz
19851_wholemount_weak.wlz
19851_wholemount_possible.wlz
19851_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19851_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thalamus mantle layer
moderate moderate
regionalmoderate expression: see section 09 10 11 13 14 15 weak expression: see section 16
cerebral cortex mantle layer
strong strong
regionalstrong expression: see section 01 02 03 04 05 21 22 23 24
cerebral cortex marginal layer
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
telencephalon mantle layer
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 19 20 21 22 weak expression: see section 07
olfactory cortex marginal layer
strong strong
regionalstrong expression: see section 09 10 11 12 14 15 16 17
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 09 10 16 17
pons mantle layer
moderate moderate
regionalmoderate expression: see section 07 08 17 18 19
glossopharyngeal ix ganglion
moderate moderate
single cellmoderate expression: see section 07 08 18 19
trigeminal v ganglion
moderate moderate
single cellmoderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 07 08 17 18 19 moderate expression: see section 06
ventral grey horn
strong strong
single cellstrong expression: see section 10 11 14 15 16
spinal cord ventricular layer
strong strong
single cellstrong expression: see section 10 11 13 14
dorsal root ganglion
moderate moderate
single cellmoderate expression: see section 09 10 14 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35111
Entity Detected:Cacna1e, calcium channel, voltage-dependent, R type, alpha 1E subunit ( MGI:106217)
Sequence:sense strand is shown

>T35111
TGTGGTAGGTAGGGCTAGGAAAAGGAGGTTCTCCAGGGCCCACAAAGGTGCCTGTAGCTCTTGTAAGGGT
AATATTAACTCCAATAGCAAGGGCCCCAACCCCAGAAGGGTTCAGCCACAGTATAGGAACTCCATGCAGT
TCTCCACCTTGGCCCTTTAGATACTCGTCAGAGGGAACCCCCAACAGTAGGGGCGAGGTAGATGGGGAGA
CTTAAGGCACTCTGGGCAGACTCAGTTTTCCAACAAACATAAAGGAACTCAGTGTTGGTGGCAGGGCTGT
GACCACAGTGGGCTGGAGGCAGGCAGCAGGCCTCTGTCAGACAGCAAGACTAATGCTAAGCCAAGCCCAG
AAAAGCCAGGTATTAGCAACTACATGTTCTCTGCCACTTCTCCAAATGGACAGGGAACACCACATCCAAG
GGGGTCACTGCGTAATGACTTCTCTACAGATGAGAAAGCCTGGTAGACTGAAATGGGAATGTTCCAAGGG
GCCTGGGATCCCCTGTGAGGAGAAGTCCTTGAGCCGTGAAGCTCATGCAGCTTTGGTTTGGGGTTTGGGG
AGTCAGTTGAAGGTACTTAAGATAATTAAGTAACTATACCTCAGTTTTGACCTGAGCTGCGGATTTTAGT
AAAATAGGCTCTGATGTGGCTCCCACTATCTCTGATATTCCCTGGGCCAGGCCTGGGTTCCATCCCTCCC
CAGAGTAAAGGATGGGTAGAAGACAACCTTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 89441. Forward Primer - name:089441_F_cDNA_Cacna1e, sequence:TGTGGTAGGTAGGGCTAGGAAA; Reverse Primer - name:089441_N_SP6_cDNA_Cacna1e, sequence:CAAGGTTGTCTTCTACCCATCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19850 same embryo
 EMAGE:19849 same embryo
 EMAGE:19848 same embryo
 EMAGE:19847 same embryo
 EurExpress:euxassay_006436 same experiment
 MGI:4823575 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS