EMAGE mouse gene expression spatial database

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EMAGE:19865

Mia3 melanoma inhibitory activity 3 ( MGI:2443183)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006460. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006460_01	euxassay_006460_02	euxassay_006460_03	euxassay_006460_04

euxassay_006460_05	euxassay_006460_06	euxassay_006460_07	euxassay_006460_08	euxassay_006460_09

euxassay_006460_10	euxassay_006460_11	euxassay_006460_12	euxassay_006460_13	euxassay_006460_14

euxassay_006460_15	euxassay_006460_16	euxassay_006460_17	euxassay_006460_18	euxassay_006460_19

euxassay_006460_20	euxassay_006460_21	euxassay_006460_22	euxassay_006460_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19865_wholemount_strong.wlz

19865_wholemount_moderate.wlz

19865_wholemount_weak.wlz

19865_wholemount_possible.wlz

19865_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19865_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

moderate

single cell

moderate expression: see section 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35768
Entity Detected:	Mia3, melanoma inhibitory activity 3 ( MGI:2443183)
Sequence:	sense strand is shown >T35768 CCACACTCAGTCGAAGAGACACTCCTAGAAGTGAATTTGGGTCACTGGACAGGCATTTACCTCGCCCTCG ATGGCCATCAGAGGCATCTGGCAAACACTCTGCTTCTGACCCGGGTCCCGCTCCTGTGGTGAACAGCAGC TCCAGGAGCTCTTCTCCCGCTAAGGCCGTGGATGAGGGCAAGGTTAATATGGCTCCCAAAGGGCCGCCTC CATTTCCAGGGGTGCCTCTCATGGGAGGCCCAGTGCCACCACCCATACGATATGGACCACCTCCCCAGCT ATGTGGTGGGCCTTTTGGGCCTCGCCCACTTCCTCCACCATTTGTTCCAGGCATGCATCCACCACTAGGC GTAAGAGAATATGCACCAGGTGTTCTGCCTGGGAAACGGGACCTGCCTCTTGATCCTCGGGAATTTTTAC CAGGACATACACCATTTAGACCTCCAGGTTCACTCGGTCCAAGAGAGTTCTTTATTCCTGGTACCCGATT ACCACCTCCAACCCATGGTCCTCAGGAATACCCACCACCACCACCTGCTGTAAGAGACTCACTGCCTTCA GGCCCGAGAGAGGAAGCCAAACCTGCCTCTCCAAGCAGTGTCCAGGACCGCTCACAGGCTTCAAAGCCCA CCCCCTGACTTTGACCTCTATCGATGAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 87191. Forward Primer - name:087191_F_cDNA_9130229H14Rik, sequence:CCACACTCAGTCGAAGAGACAC; Reverse Primer - name:087191_N_SP6_cDNA_9130229H14Rik, sequence:CTCATCGATAGAGGTCAAAGTCA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19863 same embryo
	EMAGE:19866 same embryo
	EMAGE:19864 same embryo
	EurExpress:euxassay_006460 same experiment
	MGI:4826194 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source