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EMAGE:19880

9930013L23Rik RIKEN cDNA 9930013L23 gene ( MGI:2443629)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19880 EMAGE:19880 EMAGE:19880 EMAGE:19880 EMAGE:19880
"Pseudo-wholemount" of euxassay_009291. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009291_01 euxassay_009291_02 euxassay_009291_03 euxassay_009291_04
EMAGE:19880 EMAGE:19880 EMAGE:19880 EMAGE:19880 EMAGE:19880
euxassay_009291_05 euxassay_009291_06 euxassay_009291_07 euxassay_009291_08 euxassay_009291_09
EMAGE:19880 EMAGE:19880 EMAGE:19880 EMAGE:19880 EMAGE:19880
euxassay_009291_10 euxassay_009291_11 euxassay_009291_12 euxassay_009291_13 euxassay_009291_14
EMAGE:19880 EMAGE:19880 EMAGE:19880 EMAGE:19880 EMAGE:19880
euxassay_009291_15 euxassay_009291_16 euxassay_009291_17 euxassay_009291_18 euxassay_009291_19
EMAGE:19880 EMAGE:19880 EMAGE:19880 EMAGE:19880
euxassay_009291_20 euxassay_009291_21 euxassay_009291_22 euxassay_009291_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19880Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19880_wholemount_strong.wlz
19880_wholemount_moderate.wlz
19880_wholemount_weak.wlz
19880_wholemount_possible.wlz
19880_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19880_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
cerebral cortex mantle layer
moderate moderate
single cellmoderate expression: see section 08 09 10 11 13 14 15 weak expression: see section 07 16 17 18 19
telencephalon dura mater
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 moderate expression: see section 08
dorsal root ganglion
strong strong
single cellstrong expression: see section 07 08 09 10 14 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35792
Entity Detected:9930013L23Rik, RIKEN cDNA 9930013L23 gene ( MGI:2443629)
Sequence:sense strand is shown

>T35792
AGTATCAGCCTGTCATCACCCTGCAGAAGGGCTATACTATCCACTGGGACCAGACAGCCCCCGCCGAGCT
TGCCATCTGGCTCATCAACTTCAACAAGGGTGACTGGATCCGAGTGGGGCTCTGCTACCCAAGAGGCACC
ACCTTTTCCATCCTTTCAGATGTTCACAATCGCCTGCTAAAGCAAACGTCCAAGACTGGGACCTTTGTGA
GGACTCTGCAGATGGACAAAGTTGAGCAGAGCTATCCTGGCAGGAGCCACTACTACTGGGATGAGGATTC
TGGGTTGCTCTTCTTGAAATTAAAAGCCCAGAACGAAAGGGAGAAATTTGCTTTCTGCTCCATGAAGGGC
TGTGAGAGAATCAAGATTAAAGCTCTGCTCCCAAGAAATGCAGGCATCAGCGACTGCACAGCCACGGCTT
ACCCCAGGTTCACTGAGAGGGCCATAGTGGATGTACCAATGCCCAGGAAGCTCTTTGGGGCTCAGCTGAA
GACAAAAGACCACTTCCTGGAAGTGAAGATGGAAAGTTCCAGGCAGCACTTCTTCCACCTTCGGAATGAC
TTCGCTTATATTGAAGTGGATGGGAGGAGGTACCCTTGCTCAGAAGAT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 91804. Forward Primer - name:091804_F_cDNA_9930013L23Rik, sequence:AGTATCAGCCTGTCATCACCCT; Reverse Primer - name:091804_N_SP6_cDNA_9930013L23Rik, sequence:ATCTTCTGAGCAAGGGTACCTCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19881 same embryo
 EMAGE:19883 same embryo
 EMAGE:19882 same embryo
 EurExpress:euxassay_009291 same experiment
 MGI:4822812 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS